Diagnosis Confirmation of Inclusion Bodies Hepatitis on Broiler Chicken using Polymerase Chain Reaction Method

J. Rahmahani, Maha Kirana, F. Rantam, S. Suwarno
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引用次数: 1

Abstract

This study aimed to diagnose cases of Inclusion Bodies Hepatitis (IBH) in broiler chickens. A total of 6 chickens were collected from farms in Banten and Jember. Samples were taken from the liver which underwent changes such as brittle, yellowish in color and enlarged. The samples were ground and extracted, then the Polymerase Chain Reaction (PCR) test was performed using Primers Hexon A (5'-CAARTTCAGRCAGACGGT-3') and Hexon B ( 5'-TAGTGATGMCGSGACATCAT-3' ) with 897 bp length. The results showed that the broiler chicken case sample from Banten was positively 100% infected by the IBH Virus.
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聚合酶链反应法诊断肉鸡包涵体肝炎
本研究旨在对肉鸡包涵体肝炎(IBH)进行诊断。从万丹和余月的农场共收集了6只鸡。样本取自肝脏,肝脏变脆、变黄、变大。样品研磨后提取,用长度为897 bp的引物Hexon A(5’- caarttcagagrcagacggt -3’)和Hexon B(5’-TAGTGATGMCGSGACATCAT-3’)进行聚合酶链反应(PCR)检测。结果表明,万腾肉鸡病例标本IBH病毒感染阳性率为100%。
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