Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.143-154
Rahmat Hidayat, O. Carman, A. Alimuddin
The phenomenon of sexual dimorphism in climbing perch, which shows that female fish grow faster than males, underlies the development of mono-sex culture. Female mono-sex culture is more applicable for farmers by crossing neo-male fish with normal females. The timing of sexual differentiation in climbing perch is still unknown. It is very useful in sex reversal procedures to produce neo-male climbing perch. This study revealed the time and status of climbing perch sexual differentiation. Ten samples of climbing perch from the spawning of five pairs of parents were taken from the nursery pond at 10–29 days post-hatching (dph). Samples were prepared through a histology preparation procedure. Observations of the structure and characteristics of the gonads were carried out using a light microscope and analyzed histologically. The results indicated that gonad samples aged 10, 11, 12, 13, 15, and 16 dph showed primordial germ cells surrounded by somatic tissue forming genital ridges and mitotic division. Meanwhile, the gonads begin to differentiate as ovaries found at 18 dph with the presence of oogonia and ovarian cavities. Gonads aged 20–21 dph increasingly showed single oogonia cells (size 20–37.5 µm), germ cell cysts, genital ridges, oocytes undergoing the vitellogenesis process, perinucleolar oocytes, and the formation of the ovarian cavity. Sex differentiation of climbing perch was predicted from 18–21 dph. This conclusion underlies that the sex reversal procedure in climbing perch must be carried out before 18 dph.
{"title":"Early Sex Differentiation of Climbing Perch (Anabas testudineus Bloch.): A Pathway to Feminization","authors":"Rahmat Hidayat, O. Carman, A. Alimuddin","doi":"10.20473/jmv.vol7.iss1.2024.143-154","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.143-154","url":null,"abstract":"The phenomenon of sexual dimorphism in climbing perch, which shows that female fish grow faster than males, underlies the development of mono-sex culture. Female mono-sex culture is more applicable for farmers by crossing neo-male fish with normal females. The timing of sexual differentiation in climbing perch is still unknown. It is very useful in sex reversal procedures to produce neo-male climbing perch. This study revealed the time and status of climbing perch sexual differentiation. Ten samples of climbing perch from the spawning of five pairs of parents were taken from the nursery pond at 10–29 days post-hatching (dph). Samples were prepared through a histology preparation procedure. Observations of the structure and characteristics of the gonads were carried out using a light microscope and analyzed histologically. The results indicated that gonad samples aged 10, 11, 12, 13, 15, and 16 dph showed primordial germ cells surrounded by somatic tissue forming genital ridges and mitotic division. Meanwhile, the gonads begin to differentiate as ovaries found at 18 dph with the presence of oogonia and ovarian cavities. Gonads aged 20–21 dph increasingly showed single oogonia cells (size 20–37.5 µm), germ cell cysts, genital ridges, oocytes undergoing the vitellogenesis process, perinucleolar oocytes, and the formation of the ovarian cavity. Sex differentiation of climbing perch was predicted from 18–21 dph. This conclusion underlies that the sex reversal procedure in climbing perch must be carried out before 18 dph.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"430 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140780478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.105-122
Junaedi Junaedi, Nurul Isnaini, M. Natsir, Trinil Susilawati
The impact of supplementing glucose, glycine, or a combination of both in Ringer’s lactate egg yolk base extender to preserve the quality of semen from local Indonesian chickens has not been previously investigated. This study aimed to examine the potential of glucose and glycine on chicken semen stored at 5°C for 120 hours. In this study, five local roosters were used. The parameters under observation included semen volume, odor, pH levels, consistency, color, mass movement, concentration, motility, viability, abnormality, plasma membrane integrity, chromatin degeneration, and acrosomal cap integrity. This study was conducted using a completely randomized design (CRD) with four treatments groups and 10 replication, i.e. T1 (control without supplementation), T2 (50 mM glucose), T3 (60 mM glycine), and T4 (a combination of 50 mM glucose and 60 mM glycine), respectively. In result, semen volume was 0.54 ± 0.17 mL/ejaculate, a milky white color, distinctive odor, thick consistency, good mass movement (++/+++), pH of 7.37 ± 0.23, motility of 91.50 ± 2.42%, plasma membrane integrity of 96.85 ± 0.96%, abnormality at 2.88 ± 0.77%, the concentration of 3.04 ± 0.3 billion/mL, and viability of 96.47 ± 1.71%. Following storage at 5°C for 120 hours, the motility, viability, abnormality, and acrosomal cap integrity of local chicken spermatozoa significantly different (p < 0.05) between T3 and T4 compared to T1 and T2 groups. Moreover, the integrity of the plasma membrane and chromatin degeneration in treatment T3 significantly different (p < 0.05) from T1, T2, and T4 groups. In conclusion, local chickens exhibited fair quality fresh semen both in macroscopic and microscopic evaluations. Furthermore, the combination of 60 mM glycine and 50 mM glucose into local chicken semen stored at 5°C for 120 hours effectively preserved motility and viability, minimized abnormality, maintained plasma membrane integrity, minimized chromatin degeneration, and retained acrosomal integrity.
{"title":"Supplementation of Glycine and Glucose into Egg Yolk Lactated Ringer Diluent on The Quality of Local Chicken Semen Stored at 5oC for 120 Hours","authors":"Junaedi Junaedi, Nurul Isnaini, M. Natsir, Trinil Susilawati","doi":"10.20473/jmv.vol7.iss1.2024.105-122","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.105-122","url":null,"abstract":"The impact of supplementing glucose, glycine, or a combination of both in Ringer’s lactate egg yolk base extender to preserve the quality of semen from local Indonesian chickens has not been previously investigated. This study aimed to examine the potential of glucose and glycine on chicken semen stored at 5°C for 120 hours. In this study, five local roosters were used. The parameters under observation included semen volume, odor, pH levels, consistency, color, mass movement, concentration, motility, viability, abnormality, plasma membrane integrity, chromatin degeneration, and acrosomal cap integrity. This study was conducted using a completely randomized design (CRD) with four treatments groups and 10 replication, i.e. T1 (control without supplementation), T2 (50 mM glucose), T3 (60 mM glycine), and T4 (a combination of 50 mM glucose and 60 mM glycine), respectively. In result, semen volume was 0.54 ± 0.17 mL/ejaculate, a milky white color, distinctive odor, thick consistency, good mass movement (++/+++), pH of 7.37 ± 0.23, motility of 91.50 ± 2.42%, plasma membrane integrity of 96.85 ± 0.96%, abnormality at 2.88 ± 0.77%, the concentration of 3.04 ± 0.3 billion/mL, and viability of 96.47 ± 1.71%. Following storage at 5°C for 120 hours, the motility, viability, abnormality, and acrosomal cap integrity of local chicken spermatozoa significantly different (p < 0.05) between T3 and T4 compared to T1 and T2 groups. Moreover, the integrity of the plasma membrane and chromatin degeneration in treatment T3 significantly different (p < 0.05) from T1, T2, and T4 groups. In conclusion, local chickens exhibited fair quality fresh semen both in macroscopic and microscopic evaluations. Furthermore, the combination of 60 mM glycine and 50 mM glucose into local chicken semen stored at 5°C for 120 hours effectively preserved motility and viability, minimized abnormality, maintained plasma membrane integrity, minimized chromatin degeneration, and retained acrosomal integrity.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140784248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.134-142
V. Hendrawan, Galuh Chandra Agustina, S. Aditya, Y. Oktanella, A. Firmawati, Dewi Mariyam
Black cumin (Nigella sativa), also referred to as black cumin, is a versatile herbal treatment that has recently grown in popularity, it contains antimicrobial, antibacterial, antifungal, anti-inflammatory, analgesic, antipyretic, antiparasitic, antihistamine, antihypertensive, antitumor, anticancer, antidiabetic, and anti-asthmatic properties are just a few of the positive and pharmacological effects of active ingredients in black cumin, such as thymoquinone and other compounds. This study aimed to evaluate black cumin activity ameliorates organophosphate toxicity in pregnant rats based on progesterone levels and fetal bone development. In this study, pregnant albino rats were divided into five treatment groups i.e. (K-) placebo; (K+) treated an organophosphate; treated an organophosphate and black cumin extract at a dose of (P1) 200 mg/kg BW, (P2) 400 mg/kg BW, and (P3) 600 mg/kg BW. The progesterone levels serum and fetal bone development were evaluated and calculated using ANOVA followed by Turkey. The results showed that P2 and P3 groups significantly increase in progesterone levels compared to K+ and P1 groups. Fetal bone was also reported increase in the growth of caudal vertebrae. In conclusion, 400 mg/kg BW of black cumin could ameliorated progesterone levels and fetal bone development in pregnant rats with organophosphate toxicity.
{"title":"Efficacy of Black Cumin (Nigella sativa) Ameliorate Organophosphate Toxicity in Pregnant Rats Based on Progesterone Levels and Fetal Bone Development","authors":"V. Hendrawan, Galuh Chandra Agustina, S. Aditya, Y. Oktanella, A. Firmawati, Dewi Mariyam","doi":"10.20473/jmv.vol7.iss1.2024.134-142","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.134-142","url":null,"abstract":"Black cumin (Nigella sativa), also referred to as black cumin, is a versatile herbal treatment that has recently grown in popularity, it contains antimicrobial, antibacterial, antifungal, anti-inflammatory, analgesic, antipyretic, antiparasitic, antihistamine, antihypertensive, antitumor, anticancer, antidiabetic, and anti-asthmatic properties are just a few of the positive and pharmacological effects of active ingredients in black cumin, such as thymoquinone and other compounds. This study aimed to evaluate black cumin activity ameliorates organophosphate toxicity in pregnant rats based on progesterone levels and fetal bone development. In this study, pregnant albino rats were divided into five treatment groups i.e. (K-) placebo; (K+) treated an organophosphate; treated an organophosphate and black cumin extract at a dose of (P1) 200 mg/kg BW, (P2) 400 mg/kg BW, and (P3) 600 mg/kg BW. The progesterone levels serum and fetal bone development were evaluated and calculated using ANOVA followed by Turkey. The results showed that P2 and P3 groups significantly increase in progesterone levels compared to K+ and P1 groups. Fetal bone was also reported increase in the growth of caudal vertebrae. In conclusion, 400 mg/kg BW of black cumin could ameliorated progesterone levels and fetal bone development in pregnant rats with organophosphate toxicity.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"16 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.88-104
Diah Artati, Moh. Oman, Supriyanto Supriyanto, D. Sukarta, A. Robisalmi
The high demand for fish consumption has an impact on increasing aquaculture productivity and causes the vulnerability of increasing bacterial populations in aquaculture fields, so more rapid handling is needed. The use of phenotypic KIT methods (API 20E) has been applied as a targeted and efficient identification support in targeting better bacterial identification accuracy but often provides unequal results. Method validation is one of the general requirements for the competence of a laboratory evaluation: to provide coherent, interpretable, and accurate results with known uncertainties. The purpose of this study was to validate the API 20E KIT method for the identification of A. hydrophila. The conventional method used as a reference is SNI 7303.1:2015. The validation parameters consisted of determining the limit of detection, sensitivity, and specificity tests, as well as the positive predictive value and negative predictive value. The results showed that the limit of the detection value of the API 20E KIT was at a concentration of 100 CFU/mL with an ID of 99.00%. The sensitivity and specificity values in the positive and negative target samples were 100% with a positive predictive value and a negative predictive value of 0%, respectively. In conclusion, the API 20E KIT method as an alternative test method or rapid test was proven valid for identifying A. hydrophila by the test results using the reference method.
对水产品消费的高需求影响了水产养殖生产力的提高，并导致水产养殖场中的细菌数量不断增加，因此需要更快速的处理方法。表型 KIT 方法（API 20E）作为一种有针对性的高效鉴定辅助手段，可提高细菌鉴定的准确性，但其结果往往不一致。方法验证是实验室评估能力的一般要求之一：在已知不确定性的情况下提供连贯、可解释和准确的结果。本研究的目的是验证 API 20E KIT 方法对蚜虫水华的鉴定效果。参照的传统方法是 SNI 7303.1:2015。验证参数包括确定检测限、灵敏度和特异性测试，以及阳性预测值和阴性预测值。结果显示，API 20E KIT 的检出限为 100 CFU/mL，ID 值为 99.00%。阳性和阴性目标样本的灵敏度和特异性值均为 100%，阳性预测值和阴性预测值分别为 0%。总之，API 20E KIT 方法作为一种替代检测方法或快速检测方法，通过使用参考方法的检测结果证明了其在鉴定嗜水蝇方面的有效性。
{"title":"Validation of Analytical Method for Aeromonas hydrophila Identification using Analytical Profile Index (API) 20E KIT Method","authors":"Diah Artati, Moh. Oman, Supriyanto Supriyanto, D. Sukarta, A. Robisalmi","doi":"10.20473/jmv.vol7.iss1.2024.88-104","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.88-104","url":null,"abstract":"The high demand for fish consumption has an impact on increasing aquaculture productivity and causes the vulnerability of increasing bacterial populations in aquaculture fields, so more rapid handling is needed. The use of phenotypic KIT methods (API 20E) has been applied as a targeted and efficient identification support in targeting better bacterial identification accuracy but often provides unequal results. Method validation is one of the general requirements for the competence of a laboratory evaluation: to provide coherent, interpretable, and accurate results with known uncertainties. The purpose of this study was to validate the API 20E KIT method for the identification of A. hydrophila. The conventional method used as a reference is SNI 7303.1:2015. The validation parameters consisted of determining the limit of detection, sensitivity, and specificity tests, as well as the positive predictive value and negative predictive value. The results showed that the limit of the detection value of the API 20E KIT was at a concentration of 100 CFU/mL with an ID of 99.00%. The sensitivity and specificity values in the positive and negative target samples were 100% with a positive predictive value and a negative predictive value of 0%, respectively. In conclusion, the API 20E KIT method as an alternative test method or rapid test was proven valid for identifying A. hydrophila by the test results using the reference method.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"25 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140763363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.41-47
Prima Ayu Wibawati, Yunia Chrismonica, Racca Biana Susanti, Mario Putra Himawan, Afaf Heny Sintya Devy, Juwita Ayu Nirmalasari, Abzal Abdramanov
This study aimed to analyze the microbial contamination of chicken meat and its pH value from traditional markets in Banyuwangi, East Java to ensure public health. A total of 30 raw chicken meat samples from three traditional markets were used in this study and examined for the presence of Escherichia coli and Salmonella spp. E. coli and Salmonella spp. were identified using Gram staining, culturing, and biochemical tests. The Most Probable Number (MPN) method was used for coliform and E. coli. Measuring the pH value of chicken meat was carried out after purchasing the meat and 24 hours after storage at cold temperatures. The results showed coliform contamination in all samples with an average of >1100 CFU/G with E. coli contamination of 20% (6/30), exceeding the maximum recommended limit. Furthermore, the identification of Salmonella spp. in all samples showed negative results. Chicken meat pH value decreased with the length of postmortem time; immediately after purchase (0 hours), the pH value was 5.78 ± 0.26, and after 24 hours of storage, it raised 6.02 ± 0.28. In conclusion, there was high coliform contamination out of 30 raw chicken meat samples taken from some traditional markets in Banyuwangi. Furthermore, E. coli contamination was as much as 20%, and Salmonella spp. was negative.
{"title":"Microbial Contamination in Chicken Meat in Traditional Markets in Banyuwangi","authors":"Prima Ayu Wibawati, Yunia Chrismonica, Racca Biana Susanti, Mario Putra Himawan, Afaf Heny Sintya Devy, Juwita Ayu Nirmalasari, Abzal Abdramanov","doi":"10.20473/jmv.vol7.iss1.2024.41-47","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.41-47","url":null,"abstract":"This study aimed to analyze the microbial contamination of chicken meat and its pH value from traditional markets in Banyuwangi, East Java to ensure public health. A total of 30 raw chicken meat samples from three traditional markets were used in this study and examined for the presence of Escherichia coli and Salmonella spp. E. coli and Salmonella spp. were identified using Gram staining, culturing, and biochemical tests. The Most Probable Number (MPN) method was used for coliform and E. coli. Measuring the pH value of chicken meat was carried out after purchasing the meat and 24 hours after storage at cold temperatures. The results showed coliform contamination in all samples with an average of >1100 CFU/G with E. coli contamination of 20% (6/30), exceeding the maximum recommended limit. Furthermore, the identification of Salmonella spp. in all samples showed negative results. Chicken meat pH value decreased with the length of postmortem time; immediately after purchase (0 hours), the pH value was 5.78 ± 0.26, and after 24 hours of storage, it raised 6.02 ± 0.28. In conclusion, there was high coliform contamination out of 30 raw chicken meat samples taken from some traditional markets in Banyuwangi. Furthermore, E. coli contamination was as much as 20%, and Salmonella spp. was negative.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"183 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140758838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.163-176
A. Falaye, Abel Abah, S. Sule
The effect of Gum Arabic (GA) on growth, carcass quality, blood parameters, gut morphometry, and organ histology of African catfish Clarias gariepinus was studied. Five isonitrogenous diets with inclusion levels of GA at 0 g/100 g (GA0; control), 5 g/100 g (GA5), 10 g/100 g (GA10), 15 g/100 g (GA15), and 20 g/100 g (GA20) were formulated in a 40% crude protein diet. Juveniles of C. gariepinus with average weight (7.35±0.48 g/fish) were stocked at 13 fish/25 L of water for 12 weeks in a completely randomized design and fed at 3% body weight daily. Growth indices showed GA10 improved the mean weight gain (69.88±8.89 g) and specific growth rate (2.76±0.14 %/day) while at (GA15); (GA20) growth was depressed. Carcass quality revealed dietary levels were superior to control with GA10 significantly different (p < 0.05) amongst treatments in protein and fat content. Hematological profile showed variations; erythrocyte indices compared to control, while plasma chemistry of aspartate aminotransferase, alkaline phosphatase, and alanine aminotransferase showed no difference (p > 0.05). Gut morphometrics varied with no particular pattern. At inclusion above GA5 the gill, liver, and kidney were affected histologically with no changes in the intestines of all treatments. Diet supplemented with gum arabic at 10 g/100 g improved indices of performance of C. gariepinus juveniles in this study.
{"title":"Effect of Gum Arabic (Acacia senegal) on Growth Performance, Carcass Quality and Health of Clarias gariepinus Juveniles","authors":"A. Falaye, Abel Abah, S. Sule","doi":"10.20473/jmv.vol7.iss1.2024.163-176","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.163-176","url":null,"abstract":"The effect of Gum Arabic (GA) on growth, carcass quality, blood parameters, gut morphometry, and organ histology of African catfish Clarias gariepinus was studied. Five isonitrogenous diets with inclusion levels of GA at 0 g/100 g (GA0; control), 5 g/100 g (GA5), 10 g/100 g (GA10), 15 g/100 g (GA15), and 20 g/100 g (GA20) were formulated in a 40% crude protein diet. Juveniles of C. gariepinus with average weight (7.35±0.48 g/fish) were stocked at 13 fish/25 L of water for 12 weeks in a completely randomized design and fed at 3% body weight daily. Growth indices showed GA10 improved the mean weight gain (69.88±8.89 g) and specific growth rate (2.76±0.14 %/day) while at (GA15); (GA20) growth was depressed. Carcass quality revealed dietary levels were superior to control with GA10 significantly different (p < 0.05) amongst treatments in protein and fat content. Hematological profile showed variations; erythrocyte indices compared to control, while plasma chemistry of aspartate aminotransferase, alkaline phosphatase, and alanine aminotransferase showed no difference (p > 0.05). Gut morphometrics varied with no particular pattern. At inclusion above GA5 the gill, liver, and kidney were affected histologically with no changes in the intestines of all treatments. Diet supplemented with gum arabic at 10 g/100 g improved indices of performance of C. gariepinus juveniles in this study.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"429 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.68-75
Gayuh Syaikhullah, R. T. Hertamawati, Muhammad Adhyatma
The purpose of this study was to determine the diversity of the growth hormone secretagogue receptor (GHSR) gene and also to analyze the association of the GHSR gene with the body proportions of Bangkok chickens. A total of 125 Bangkok chickens were reared, and blood samples were taken. Bangkok chickens were kept in colony cages with ad libitum feeding and drinking. The GHSR gene polymorphism was determined using the PCR-RFLP method. The restriction enzyme used in this study was Hin6I. The data analyzed were indicators of body proportions, which included data on body weight, carcass weight, and commercial cut weight. Single nucleotide polymorphism (SNP) identification using the Molecular Evolutionary Genetics Analysis 7 program with reference number AB095994.1. Allele frequency values, genotype frequencies, and Hardy-Weinberg balance values were also analyzed. The association between the GHSR gene and the traits observed in Bangkok chickens was analyzed using the T-test. The results of the study showed that the GHSR gene in Bangkok chickens had two genotypes, namely TT and CT. The values for Ho and He were 0.224 and 0.198, respectively. The genotype frequencies of TT and CT were 0.776 and 0.224, respectively. The two genotypes were associated with body weight, carcass weight, and commercial weight (p < 0.05). For all measured criteria, the TT genotype showed a higher weight value than the CT genotype. The GHSR gene has the potential to be used as a genetic marker for the selection process on body weight, carcass weight, and commercial weight traits.
{"title":"Exploring the Relationship Between Growth Hormone Secretagogue Receptor (GHSR) Gene and Body Proportions in Bangkok Chickens: Insights from DNA Sequencing and Hin6I Enzyme-Restricted PCR-RFLP Analysis","authors":"Gayuh Syaikhullah, R. T. Hertamawati, Muhammad Adhyatma","doi":"10.20473/jmv.vol7.iss1.2024.68-75","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.68-75","url":null,"abstract":"The purpose of this study was to determine the diversity of the growth hormone secretagogue receptor (GHSR) gene and also to analyze the association of the GHSR gene with the body proportions of Bangkok chickens. A total of 125 Bangkok chickens were reared, and blood samples were taken. Bangkok chickens were kept in colony cages with ad libitum feeding and drinking. The GHSR gene polymorphism was determined using the PCR-RFLP method. The restriction enzyme used in this study was Hin6I. The data analyzed were indicators of body proportions, which included data on body weight, carcass weight, and commercial cut weight. Single nucleotide polymorphism (SNP) identification using the Molecular Evolutionary Genetics Analysis 7 program with reference number AB095994.1. Allele frequency values, genotype frequencies, and Hardy-Weinberg balance values were also analyzed. The association between the GHSR gene and the traits observed in Bangkok chickens was analyzed using the T-test. The results of the study showed that the GHSR gene in Bangkok chickens had two genotypes, namely TT and CT. The values for Ho and He were 0.224 and 0.198, respectively. The genotype frequencies of TT and CT were 0.776 and 0.224, respectively. The two genotypes were associated with body weight, carcass weight, and commercial weight (p < 0.05). For all measured criteria, the TT genotype showed a higher weight value than the CT genotype. The GHSR gene has the potential to be used as a genetic marker for the selection process on body weight, carcass weight, and commercial weight traits.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"94 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140789009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.1-6
Diena Delaiah, Ahmad Aswin, Helen Susilowati, Andi Yasmin Wijaya, Firdausy Kurnia Maulana, D. Diyantoro, Watchareewan Rodprasert, Yulianna Puspitasari, Y. Dhamayanti, S. Kuncorojakti
Vero cell culture as a platform for producing viral vaccines is an established and standardized process in vaccine manufacture. Generally, Vero cell culture requires media as a source of nutrition with serum supplementation to provide growth factors. However, the serum has several disadvantages including batch to batch variation and adventitious agent. Therefore, chemically defined serum-free media (SFM) are formulated by using standardized growth factors. Epidermal Growth Factor (EGF) is one of growth factors that showed adequate mitogenic support in serum-free medium system, especially in Vero cells. In this study, SFM-EGF media was compared with serum supplementation media, namely MEM 10% and MEM 5% FBS supplementation. The cell morphology was observed using an inverted microscope and their proliferation was evaluated using a MTT colorimetric-based assay. Vero E6 and Vero CCL-81 cells morphology did not show any morphological changes. Vero E6 and Vero CCL-81 proliferation in SFM-EGF media on day one to four did not show a significant difference compared to MEM 10% or MEM 5% serum supplementation media. However, the OD values of both Vero E6 and Vero CCL-81 cells given SFM-EGF media produced an average value below MEM 10% but higher than MEM 5% FBS supplementation. As such, this study proved that utilizing SFM-EGF could support the proliferation of Vero E6 and Vero CCL-81 cells.
将 Vero 细胞培养作为生产病毒疫苗的平台，是疫苗生产中一个成熟的标准化流程。一般来说，Vero 细胞培养需要培养基作为营养源，并补充血清以提供生长因子。然而，血清有几个缺点，包括批次间的差异和偶联剂。因此，人们使用标准化的生长因子配制化学定义的无血清培养基（SFM）。表皮生长因子（EGF）是一种在无血清培养基系统中显示出充分有丝分裂支持的生长因子，尤其是在 Vero 细胞中。在本研究中，SFM-EGF 培养基与补充血清的培养基（即 MEM 10%和 MEM 5% FBS）进行了比较。细胞形态用倒置显微镜观察，细胞增殖用 MTT 比色法评估。Vero E6 和 Vero CCL-81 细胞形态未发生任何变化。第 1-4 天，Vero E6 和 Vero CCL-81 在 SFM-EGF 培养基中的增殖与在 MEM 10% 或 MEM 5% 血清补充培养基中的增殖相比没有显著差异。不过，给予 SFM-EGF 培养基的 Vero E6 和 Vero CCL-81 细胞的 OD 值平均低于 MEM 10%，但高于补充 5% FBS 的 MEM。因此，这项研究证明，使用 SFM-EGF 可以促进 Vero E6 和 Vero CCL-81 细胞的增殖。
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Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.7-18
G. Syahbirin, Kurnia Alysia Aditianingrum, Kusdiantoro Mohamad
Curcuma zedoaria Rosc (Zingiberaceae), known as white turmeric or temu putih, is commonly used as traditional medicine in Indonesia. The toxicity of this herb needs to be studied to improve its application. This study aimed to evaluate the toxicity of the crude ethanol extract of temu putih rhizome against brine shrimp (Artemia salina) larvae and zebrafish (Danio rerio) embryos and to analyze the potential active compounds contained in the extract. The crude ethanol extract showed a 50% lethal concentration value (LC50) at 588 ppm against brine shrimp larvae and 224 ppm against zebrafish embryos. Based on the gas chromatography-mass spectrometry analysis, the suspected active compounds that play a role in the toxicity were epicurzerenone, curzerene, and curzerenone, while 2,4,6-trimethylacetophenone was the predominant compound.
姜黄（Zingiberaceae），又称白姜黄或 temu putih，是印度尼西亚常用的传统药材。需要对这种草药的毒性进行研究，以改进其应用。本研究旨在评估白姜黄根茎粗乙醇提取物对盐水虾（Artemia salina）幼虫和斑马鱼（Danio rerio）胚胎的毒性，并分析提取物中潜在的活性化合物。粗乙醇提取物对卤虫幼体的致死浓度值（LC50）为 588 ppm，对斑马鱼胚胎的致死浓度值（LC50）为 224 ppm。根据气相色谱-质谱分析，在毒性中起作用的可疑活性化合物为表莪术酮、莪术烯和莪术酮，而 2,4,6-三甲基苯乙酮是主要化合物。
{"title":"Acute Toxicity of Ethanol Extract of Curcuma zedoaria Rosc (Zingiberaceae) Rhizomes on Brine Shrimp Larvae and Zebrafish Embryos","authors":"G. Syahbirin, Kurnia Alysia Aditianingrum, Kusdiantoro Mohamad","doi":"10.20473/jmv.vol7.iss1.2024.7-18","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.7-18","url":null,"abstract":"Curcuma zedoaria Rosc (Zingiberaceae), known as white turmeric or temu putih, is commonly used as traditional medicine in Indonesia. The toxicity of this herb needs to be studied to improve its application. This study aimed to evaluate the toxicity of the crude ethanol extract of temu putih rhizome against brine shrimp (Artemia salina) larvae and zebrafish (Danio rerio) embryos and to analyze the potential active compounds contained in the extract. The crude ethanol extract showed a 50% lethal concentration value (LC50) at 588 ppm against brine shrimp larvae and 224 ppm against zebrafish embryos. Based on the gas chromatography-mass spectrometry analysis, the suspected active compounds that play a role in the toxicity were epicurzerenone, curzerene, and curzerenone, while 2,4,6-trimethylacetophenone was the predominant compound.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"168 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140790586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.20473/jmv.vol7.iss1.2024.33-40
Nur Diyana Mohamad Tahir, M. Matori, Han Ming Gan
Striped snakehead fingerlings Channa striata reared in a concrete pond with size 13.5 m3 (3 m ´ 3 m ´ 1.5 m) in a hatchery farm, in Labu, Negeri Sembilan, Malaysia had more than 70% mortality. The pond contained 1500 fingerlings of 5–7 cm in total length (TL). The purpose of this study was to investigate the high mortality event on the farm as part of a veterinary diagnostics service. Clinical signs observed were skin discoloration, lethargy, and anorexia. Necropsy was performed in 10 fish with gross lesions of 1–2 mm and multiple whitish nodules in the abdominal organs and most notably in the liver, and kidney. Following necropsy, bacteriology samples from the kidney and liver were inoculated onto Tryptic Soy Agar (TSA) and Blood Agar (BA). Pure colonies were observed and were Gram-negative with short rod morphology. The full-length 16S rRNA gene was amplified from the pure colonies followed by sequencing using the Nanopore MinION™ (Oxford Nanopore, UK). The isolated strain was identified as Aeromonas schubertii based on the assembled 16S rRNA sequence showing nucleotide identity of 99.73% (top hit) to A. schubertii (GenBank Accession: NR_037014.2) while only a nucleotide identity of 97.55% was observed against Aeromonas hydrophila (GenBank Accession: NR_119190.1-), respectively. Histopathological examination from the liver, kidney, spleen, and intestine revealed granulomatous nephritis, and degeneration of tubular epithelium, with granulomatous hepatitis, splenitis, and enteritis. The prognosis of this case was grave. No treatment was given as the owner had decided to dispose of all current stock. This is the first A. schubertii infection in C. striata described in Malaysia. The findings of the present study may serve as a reference for similar cases in C. striata in the future so rapid diagnosis and treatment can be made if required.
{"title":"First Report of Aeromonas schubertii Infection in Striped Snakehead Channa striata (Bloch, 1793) Fingerlings in Malaysia","authors":"Nur Diyana Mohamad Tahir, M. Matori, Han Ming Gan","doi":"10.20473/jmv.vol7.iss1.2024.33-40","DOIUrl":"https://doi.org/10.20473/jmv.vol7.iss1.2024.33-40","url":null,"abstract":"Striped snakehead fingerlings Channa striata reared in a concrete pond with size 13.5 m3 (3 m ´ 3 m ´ 1.5 m) in a hatchery farm, in Labu, Negeri Sembilan, Malaysia had more than 70% mortality. The pond contained 1500 fingerlings of 5–7 cm in total length (TL). The purpose of this study was to investigate the high mortality event on the farm as part of a veterinary diagnostics service. Clinical signs observed were skin discoloration, lethargy, and anorexia. Necropsy was performed in 10 fish with gross lesions of 1–2 mm and multiple whitish nodules in the abdominal organs and most notably in the liver, and kidney. Following necropsy, bacteriology samples from the kidney and liver were inoculated onto Tryptic Soy Agar (TSA) and Blood Agar (BA). Pure colonies were observed and were Gram-negative with short rod morphology. The full-length 16S rRNA gene was amplified from the pure colonies followed by sequencing using the Nanopore MinION™ (Oxford Nanopore, UK). The isolated strain was identified as Aeromonas schubertii based on the assembled 16S rRNA sequence showing nucleotide identity of 99.73% (top hit) to A. schubertii (GenBank Accession: NR_037014.2) while only a nucleotide identity of 97.55% was observed against Aeromonas hydrophila (GenBank Accession: NR_119190.1-), respectively. Histopathological examination from the liver, kidney, spleen, and intestine revealed granulomatous nephritis, and degeneration of tubular epithelium, with granulomatous hepatitis, splenitis, and enteritis. The prognosis of this case was grave. No treatment was given as the owner had decided to dispose of all current stock. This is the first A. schubertii infection in C. striata described in Malaysia. The findings of the present study may serve as a reference for similar cases in C. striata in the future so rapid diagnosis and treatment can be made if required.","PeriodicalId":17972,"journal":{"name":"Jurnal Medik Veteriner","volume":"470 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140785918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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