Development and Testing of Trial Samples for Specific Serodiagnosis of Fasciola gigantica via External Quality Assessment

V. Q. Huy, T. T. Trung, H. M. Tuan, V. T. Thanh, Le Van Chuong, Nguyen Lam Duc Vu, Huynh Thi Diem Phuc, B. Q. Sang
{"title":"Development and Testing of Trial Samples for Specific Serodiagnosis of Fasciola gigantica via External Quality Assessment","authors":"V. Q. Huy, T. T. Trung, H. M. Tuan, V. T. Thanh, Le Van Chuong, Nguyen Lam Duc Vu, Huynh Thi Diem Phuc, B. Q. Sang","doi":"10.35248/2155-9597.1000351","DOIUrl":null,"url":null,"abstract":"Objective: To build a procedure for production of samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via an external quality assessment scheme, and to evaluate the homogeneity and stability of the trial samples. Methods: In this experimental study, samples contained an anti-Fasciola gigantica antibody according to screening by ELISA, followed by confirmation by Western blotting were collected. All samples were tested and found negative for other helminth, especially of trematode antibodies. The samples were also negative for anti-HIV-1 and -2 antibodies, anti-HCV antibodies, and HBs antigen. The samples were prepared by freeze-drying and freezing methods, the stability and homogeneity were evaluated each 2, 4, 8, 12 and up to 24 weeks. Results: We produced three lots of serum samples containing anti-Fasciola gigantica antibodies at three levels. Lot DK1 had optical density, OD=0.350 ± 0.037; Lots DK3 and DL3 had O=0.653 ± 0.046, and Lot DL1 had OD=0.850 ± 0.047 with the wavelength of 450 nm. The specific IgG antibodies against Fasciola gigantica antigens were found to be positive for all three proteins, 8–9 kDa protein (P 8-9), 28 kDa (P 28), and 42 kDa (P 42) by the Western blot technique. The trial samples were confirmed to be homogeneous by Fisher’s test (F statistics0.05) as well as to be stable during 24 weeks (with t statistics0.05). Conclusion: Trial samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via external quality assessment can be produced with homogeneity and stability lasting for 24 weeks by freeze-drying and freezing methods.","PeriodicalId":15045,"journal":{"name":"Journal of Bacteriology & Parasitology","volume":"53 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bacteriology & Parasitology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35248/2155-9597.1000351","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Objective: To build a procedure for production of samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via an external quality assessment scheme, and to evaluate the homogeneity and stability of the trial samples. Methods: In this experimental study, samples contained an anti-Fasciola gigantica antibody according to screening by ELISA, followed by confirmation by Western blotting were collected. All samples were tested and found negative for other helminth, especially of trematode antibodies. The samples were also negative for anti-HIV-1 and -2 antibodies, anti-HCV antibodies, and HBs antigen. The samples were prepared by freeze-drying and freezing methods, the stability and homogeneity were evaluated each 2, 4, 8, 12 and up to 24 weeks. Results: We produced three lots of serum samples containing anti-Fasciola gigantica antibodies at three levels. Lot DK1 had optical density, OD=0.350 ± 0.037; Lots DK3 and DL3 had O=0.653 ± 0.046, and Lot DL1 had OD=0.850 ± 0.047 with the wavelength of 450 nm. The specific IgG antibodies against Fasciola gigantica antigens were found to be positive for all three proteins, 8–9 kDa protein (P 8-9), 28 kDa (P 28), and 42 kDa (P 42) by the Western blot technique. The trial samples were confirmed to be homogeneous by Fisher’s test (F statistics0.05) as well as to be stable during 24 weeks (with t statistics0.05). Conclusion: Trial samples for specific serodiagnosis of anti-Fasciola gigantica antibodies via external quality assessment can be produced with homogeneity and stability lasting for 24 weeks by freeze-drying and freezing methods.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
通过外部质量评估进行巨型片形吸虫特异性血清诊断的试验样品的开发和测试
目的:建立一套采用外部质量评价方案的巨型片形吸虫抗体特异性血清诊断样品的生产流程,并对样品的均匀性和稳定性进行评价。方法:采用ELISA法筛选含有巨型片形吸虫抗抗体的样品,经免疫印迹法确认。对所有样本进行了检测,发现其他蠕虫呈阴性,尤其是吸虫抗体。抗hiv -1和-2抗体、抗hcv抗体和HBs抗原均呈阴性。采用冷冻干燥和冷冻法制备样品,每2周、4周、8周、12周和24周对样品进行稳定性和均匀性评价。结果:我们生产了3批含有3个水平的巨型片形吸虫抗体的血清样品。Lot DK1光密度,OD=0.350±0.037;批次DK3和DL3的OD值为0.653±0.046,批次DL1的OD值为0.850±0.047,波长为450 nm。Western blot检测发现,8-9 kDa蛋白(p8 -9)、28 kDa蛋白(p28)和42 kDa蛋白(p42)的IgG抗体均呈阳性。试验样品经Fisher检验证实均质(F有统计学意义0.05),24周内均稳定(t有统计学意义0.05)。结论:采用冷冻干燥法和冷冻法制备的巨型片形吸虫抗体特异性血清诊断试验样品具有均匀性和24周的稳定性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Antigenic Structural Similarity as a Predictor for Antibody Cross-Reactivity What is the Probability that a Vaccinated Person is Shielded from COVID-19? Severe Rhinocerebral Mucormycosis Case Developed after COVID-19 Prevalence and Epidemiology of Gastrointestinal Parasites in Cattle in Different Zones of Tehsil Chakwal, Punjab, Pakistan Characterization, Associated Risk Factors and Possible Treatment of Healthcare Associated Methicillin-Resistant Staphylococcus aureus (HA-MRSA) and Community-Associated Methicillin-Resistant Staphylococcus aureus (CA-MRSA)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1