The in vitro Evaluation of Protective Effect of Rutin on Acrylamide Induced Oxidative Aging in NIH3T3 Cells

Abstract

Background: Aging is one of the important factor in the development of age-related diseases. Acrylamide (ACR) can be produced during rich-carbohydrate foods preparing at high temperature. Recently, studies showed that ACR can induce cellular aging. Furthermore, Rutin is a natural flavonoid, which possesses a potent antioxidant activity. Objective: In this study, ptotective effect of Rutin (a potent antioxidant) on oxidative-induced aging after ACR exposure was evaluated. Materials and Methods: NIH3T3 mouse embryonic fibroblast cells were used for this study, which treated by different concentrations of ACR and H2O2. Oxidative stress was assessed by measuring of malondialdehyde and glutathion concentrations. To evaluate of the aging process, β-galactosidase activity was evaluated by enzyme staining and β-galactosidase activity assay kit. MTT assay was used to evaluate the viability of the cells. Results: Exposure of cells to ACR and H2O2 significantly decreased the cell viability and using of rutin significantly improved cell viability in cells treated by ACR (P<0.05). Rutin increased glutathion level in cells treated by ACR and H2O2 (P<0.05). Also, the rate of lipid peroxidation was significantly lower in the group treated with rutin and H2O2 than in the H2O2 group (P<0.05).The β -galactosidase activity was increased in H2O2 and ACR groups. The using of rutin with acrylamide significantly reduced the activity of β-galactosidase enzyme compared to acrylamide group (P<0.05). Conclusion: Based on this study, oxidative stress may be presented as an important mechanism in ACR induced-aging in NIH3T3 cells. On the other hand, Rutin, as a potent antioxidant, decresead aging by inhibition of oxidative damage.