Purification and characterisation of phytochemicals extracted from Rhizophora mucronata: Their efficacy against Pseudomonas aeruginosa infection in Catla catla

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL Reviews in Analytical Chemistry Pub Date : 2022-01-01 DOI:10.1515/revac-2022-0050
Gurram Beulah, Divya Deepthimahanthi, G. Simhachalam, A. D. Chintagunta, M. Sravya, N. S. S. Kumar
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引用次数: 2

Abstract

Abstract The aim of this work is to purify and characterise bioactive molecules from various solvent extracts prepared with the leaves of Rhizophora mucronata, a mangrove plant. In vitro assessment of antibacterial activity of n-hexane, diethyl ether, ethanol, and aqueous extracts (AEs) against Bacillus subtilis (0.4 ± 0.1, 1.1 ± 0.3, 0.9 ± 0.1, and 1.2 ± 0.7 mm), Staphylococcus aureus (1.2 ± 0.1, 2.3 ± 0.5, 3.2 ± 1.2, and 4.4 ± 1.7 mm), Klebsiella pneumoniae (0.5 ± 0.3, 1.7 ± 0.4, 3.9 ± 1.4, and 3.9 ± 0.9 mm), and Pseudomonas aeruginosa (0.2 ± 0.1, 2.5 ± 0.6, 3.7 ± 0.5, and 4.2 ± 1.3 mm) has proven to act as potent similar to that of commercial antibiotic. Corresponding to antibacterial activity result, antioxidant activity was also higher for AE (75.97% ± 0.5%) compared to remaining extracts (73.77% ± 2.5%, 69.99% ± 0.4%, and 65.24% ± 1.8%) at a concentration of 500 μg·mL−1. Considering both the activities, AE was selected for separation of bioactive molecule by deploying two purification techniques – column chromatography and preparative thin layer chromatography consecutively. After purification, the sample was injected into mass spectrometer and the presence of N-p-coumaroyltyramine and luteolin with excellent antibacterial and antioxidant properties (13.1 ± 2.2 mm and 80.9% ± 2.3%, respectively) were recognised. Further, in vivo investigations on Catla catla fingerlings infected with P. aeruginosa indicated that partially purified extracts cured the infection and resulted in a 100% survival rate.
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毛根霉植物化学物质的纯化及特性研究:对绿脓杆菌感染的防治作用
摘要:本研究的目的是纯化和表征红树植物Rhizophora mucronata叶片的各种溶剂提取物的生物活性分子。体外抗菌活性的评估正己烷、乙醚、乙醇和水提取物对枯草芽孢杆菌(AEs)(0.4±0.1,1.1±0.3,0.9±0.1,1.2±0.7毫米),金黄色葡萄球菌(1.2±0.1,2.3±0.5,3.2±1.2,4.4±1.7毫米),肺炎克雷伯菌(0.5±0.3,1.7±0.4,3.9±1.4,3.9±0.9毫米),和铜绿假单胞菌(0.2±0.1,2.5±0.6,3.7±0.5,4.2±1.3毫米)已被证明作为强有力的类似于商业抗生素。与抗菌活性结果相对应,在500 μg·mL−1浓度下,AE的抗氧化活性(75.97%±0.5%)高于其余提取物(73.77%±2.5%,69.99%±0.4%和65.24%±1.8%)。考虑到这两种活性,选择AE分别采用柱层析和制备薄层析两种纯化技术分离生物活性分子。纯化后的样品经质谱联用,鉴定出n -p- coumaroyylyramine和木犀草素具有良好的抗菌和抗氧化性能(分别为13.1±2.2 mm和80.9%±2.3%)。此外,对感染P. aeruginosa的Catla Catla幼鱼的体内研究表明,部分纯化的提取物治愈了感染,并导致100%的存活率。
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来源期刊
Reviews in Analytical Chemistry
Reviews in Analytical Chemistry 化学-分析化学
CiteScore
7.50
自引率
0.00%
发文量
15
审稿时长
>12 weeks
期刊介绍: Reviews in Analytical Chemistry publishes authoritative reviews by leading experts in the dynamic field of chemical analysis. The subjects can encompass all branches of modern analytical chemistry such as spectroscopy, chromatography, mass spectrometry, electrochemistry and trace analysis and their applications to areas such as environmental control, pharmaceutical industry, automation and other relevant areas. Review articles bring the expert up to date in a concise manner and provide researchers an overview of new techniques and methods.
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