The side activities of d-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating) from rabbit muscle: NADH-X formation

Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation Pub Date : 1966-10-17 DOI:10.1016/0926-6593(66)90143-3

A.G. Hilvers, J.H.M. Weenen, K. Van Dam

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引用次数: 13

Abstract

1.
1. The formation of NADH-X from NADH in the presence of glyceraldehyde phosphate dehydrogenase (EC 1.2.1.12) at low pH is observed not only in the presence of pyrophosphate, citrate or phosphate, but also in the presence of arsenate.
2.
2. Stimulation of NADH-X formation by acetyl phosphate is dependent on the presence of enzyme-bound NAD⁺; however, added NAD⁺ is inhibitory.
3.
3. Inhibition of NADH-X formation by sulphydryl reagents is prevented by acetyl phosphate in the presence of pyrophosphate or citrate, but not in the presence of arsenate or phosphate.
4.
4. In the absence of other polyvalent anions, acetyl phosphate alone can stimulate the conversion of NADH into NADH-X at low pH.
5.
5. In the presence of acetyl phosphate, the relative rate of formation of NAD⁺ and NADH-X from NADH is dependent on the pH and the anions present.

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d-甘油醛-3-磷酸的副活性:NAD+氧化还原酶(磷酸化)从兔肌肉:NADH-X的形成

1.1. 在低pH下，在甘油醛磷酸脱氢酶(EC 1.2.1.12)的存在下，NADH从NADH生成NADH- x不仅在焦磷酸盐、柠檬酸盐或磷酸盐存在下观察到，而且在砷酸盐存在下也观察到。乙酰磷酸对NADH-X形成的刺激依赖于酶结合的NAD+的存在;然而，添加的NAD+具有抑制作用。在焦磷酸盐或柠檬酸盐存在的情况下，乙酰磷酸可以阻止巯基试剂对NADH-X形成的抑制，但在砷酸盐或磷酸盐存在的情况下则不能。在没有其他多价阴离子的情况下，仅乙酰磷酸就能在低ph - 5.5下刺激NADH转化为NADH- x。在乙酰磷酸存在的情况下，NADH生成NAD+和NADH- x的相对速率取决于pH值和存在的阴离子。

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Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation

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