{"title":"A bacterial symbiont in the gill of the marine scallop <i>Argopecten irradians irradians</i> metabolizes dimethylsulfoniopropionate.","authors":"Yi Shu, Yongming Wang, Zhongcheng Wei, Ning Gao, Shuyan Wang, Chun-Yang Li, Qiang Xing, Xiaoli Hu, Xiao-Hua Zhang, Yu-Zhong Zhang, Weipeng Zhang, Zhenmin Bao, Wei Ding","doi":"10.1002/mlf2.12072","DOIUrl":null,"url":null,"abstract":"<p><p>Microbial lysis of dimethylsulfoniopropionate (DMSP) is a key step in marine organic sulfur cycling and has been recently demonstrated to play an important role in mediating interactions between bacteria, algae, and zooplankton. To date, microbes that have been found to lyse DMSP are largely confined to free-living and surface-attached bacteria. In this study, we report for the first time that a symbiont (termed \"<i>Rhodobiaceae</i> bacterium HWgs001\") in the gill of the marine scallop <i>Argopecten irradians irradians</i> can lyse and metabolize DMSP. Analysis of 16S rRNA gene sequences suggested that HWgs001 accounted for up to 93% of the gill microbiota. Microscopic observations suggested that HWgs001 lived within the gill tissue. Unlike symbionts of other bivalves, HWgs001 belongs to <i>Alphaproteobacteria</i> rather than <i>Gammaproteobacteria</i>, and no genes for carbon fixation were identified in its small genome. Moreover, HWgs001 was found to possess a <i>dddP</i> gene, responsible for the lysis of DMSP to acrylate. The enzymatic activity of <i>dddP</i> was confirmed using the heterologous expression, and in situ transcription of the gene in scallop gill tissues was demonstrated using reverse-transcription PCR. Together, these results revealed a taxonomically and functionally unique symbiont, which represents the first-documented DMSP-metabolizing symbiont likely to play significant roles in coastal marine ecosystems.</p>","PeriodicalId":9483,"journal":{"name":"Canadian Journal of Forest Research","volume":"34 1","pages":"178-189"},"PeriodicalIF":1.7000,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10989825/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Forest Research","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1002/mlf2.12072","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/6/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"FORESTRY","Score":null,"Total":0}
引用次数: 0
Abstract
Microbial lysis of dimethylsulfoniopropionate (DMSP) is a key step in marine organic sulfur cycling and has been recently demonstrated to play an important role in mediating interactions between bacteria, algae, and zooplankton. To date, microbes that have been found to lyse DMSP are largely confined to free-living and surface-attached bacteria. In this study, we report for the first time that a symbiont (termed "Rhodobiaceae bacterium HWgs001") in the gill of the marine scallop Argopecten irradians irradians can lyse and metabolize DMSP. Analysis of 16S rRNA gene sequences suggested that HWgs001 accounted for up to 93% of the gill microbiota. Microscopic observations suggested that HWgs001 lived within the gill tissue. Unlike symbionts of other bivalves, HWgs001 belongs to Alphaproteobacteria rather than Gammaproteobacteria, and no genes for carbon fixation were identified in its small genome. Moreover, HWgs001 was found to possess a dddP gene, responsible for the lysis of DMSP to acrylate. The enzymatic activity of dddP was confirmed using the heterologous expression, and in situ transcription of the gene in scallop gill tissues was demonstrated using reverse-transcription PCR. Together, these results revealed a taxonomically and functionally unique symbiont, which represents the first-documented DMSP-metabolizing symbiont likely to play significant roles in coastal marine ecosystems.
期刊介绍:
Published since 1971, the Canadian Journal of Forest Research is a monthly journal that features articles, reviews, notes and concept papers on a broad spectrum of forest sciences, including biometrics, conservation, disturbances, ecology, economics, entomology, genetics, hydrology, management, nutrient cycling, pathology, physiology, remote sensing, silviculture, social sciences, soils, stand dynamics, and wood science, all in relation to the understanding or management of ecosystem services. It also publishes special issues dedicated to a topic of current interest.