A bacterial symbiont in the gill of the marine scallop Argopecten irradians irradians metabolizes dimethylsulfoniopropionate.

IF 1.7 3区 农林科学 Q2 FORESTRY Canadian Journal of Forest Research Pub Date : 2023-06-26 eCollection Date: 2023-06-01 DOI:10.1002/mlf2.12072
Yi Shu, Yongming Wang, Zhongcheng Wei, Ning Gao, Shuyan Wang, Chun-Yang Li, Qiang Xing, Xiaoli Hu, Xiao-Hua Zhang, Yu-Zhong Zhang, Weipeng Zhang, Zhenmin Bao, Wei Ding
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Abstract

Microbial lysis of dimethylsulfoniopropionate (DMSP) is a key step in marine organic sulfur cycling and has been recently demonstrated to play an important role in mediating interactions between bacteria, algae, and zooplankton. To date, microbes that have been found to lyse DMSP are largely confined to free-living and surface-attached bacteria. In this study, we report for the first time that a symbiont (termed "Rhodobiaceae bacterium HWgs001") in the gill of the marine scallop Argopecten irradians irradians can lyse and metabolize DMSP. Analysis of 16S rRNA gene sequences suggested that HWgs001 accounted for up to 93% of the gill microbiota. Microscopic observations suggested that HWgs001 lived within the gill tissue. Unlike symbionts of other bivalves, HWgs001 belongs to Alphaproteobacteria rather than Gammaproteobacteria, and no genes for carbon fixation were identified in its small genome. Moreover, HWgs001 was found to possess a dddP gene, responsible for the lysis of DMSP to acrylate. The enzymatic activity of dddP was confirmed using the heterologous expression, and in situ transcription of the gene in scallop gill tissues was demonstrated using reverse-transcription PCR. Together, these results revealed a taxonomically and functionally unique symbiont, which represents the first-documented DMSP-metabolizing symbiont likely to play significant roles in coastal marine ecosystems.

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海洋扇贝 Argopecten irradians irradians 鳃中的一种细菌共生体代谢二甲基硫代丙酸盐。
微生物裂解二甲基硫代丙酸盐(DMSP)是海洋有机硫循环的一个关键步骤,最近的研究表明,它在调解细菌、藻类和浮游动物之间的相互作用方面发挥着重要作用。迄今为止,已发现的能溶解 DMSP 的微生物大多局限于自由生活的细菌和表面附着细菌。在本研究中,我们首次报道了海洋扇贝虹彩扇贝(Argopecten irradians irradians)鳃中的一种共生菌(称为 "Rhodobiaceae bacterium HWgs001")能够溶解和代谢 DMSP。16S rRNA 基因序列分析表明,HWgs001 占鳃微生物群的 93%。显微镜观察表明,HWgs001 生活在鳃组织内。与其他双壳类动物的共生体不同,HWgs001属于Alphaproteobacteria而非Gammaproteobacteria,在其较小的基因组中没有发现碳固定的基因。此外,还发现 HWgs001 拥有一个 dddP 基因,负责将 DMSP 分解为丙烯酸酯。利用异源表达证实了 dddP 的酶活性,并利用反转录 PCR 技术证明了该基因在扇贝鳃组织中的原位转录。这些结果共同揭示了一种在分类和功能上独特的共生生物,它代表了第一种有记载的DMSP代谢共生生物,可能在沿海海洋生态系统中发挥重要作用。
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来源期刊
CiteScore
4.20
自引率
9.10%
发文量
109
审稿时长
3 months
期刊介绍: Published since 1971, the Canadian Journal of Forest Research is a monthly journal that features articles, reviews, notes and concept papers on a broad spectrum of forest sciences, including biometrics, conservation, disturbances, ecology, economics, entomology, genetics, hydrology, management, nutrient cycling, pathology, physiology, remote sensing, silviculture, social sciences, soils, stand dynamics, and wood science, all in relation to the understanding or management of ecosystem services. It also publishes special issues dedicated to a topic of current interest.
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