A Temperature‐Dependent Index of Mitotic Interval (τ0) for Chromosome Manipulation in Paddlefish and Shovelnose Sturgeon

The Progressive Fish-culturist Pub Date : 1997-07-01 DOI:10.1577/1548-8640(1997)059<0229:ATDIOM>2.3.CO;2

W. Shelton, S. Mims, J. Clark, Ana E. Hiott, Changzheng Wang

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引用次数: 36

Abstract

Abstract A temperature-dependent measure of the mitotic interval (τ0) can help standardize chromosome manipulation in fish eggs. A tau unit (τ0) is the duration in minutes of one mitotic cycle during synchronous embryonic cleavage. It is measured over a range of temperatures, and the resulting relationship of τ0 to temperature can be used to anticipiate developmental events that are affected by temperature. Optimum induction of chromosome manipulation requires development of a specific treatment of egg shocking for each species, Timing of shock is a critical variable, but pretreatment incubation temperature affects the rate of development and thus the optimum absolute time for shocking. Mitotic intervals (τ0) are reliable indicators of developmental rates over normal temperatures for egg incubation, and thus can be used to estimate optimal times for chromosome manipulation, Mitotic intervals for paddlefish Polyodon spathula and shovelnose sturgeon Scaphirhynchus platorynchus were estimated by averaging th...

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白鲟和铲鼻鲟染色体操作有丝分裂间隔(τ0)的温度依赖指数

有丝分裂间隔(τ0)的温度依赖性测量有助于标准化鱼卵的染色体操作。τ单位(τ0)是胚胎同步分裂过程中一个有丝分裂周期的持续时间(以分钟为单位)。它是在一个温度范围内测量的，由此得到的τ0与温度的关系可以用来预测受温度影响的发育事件。染色体操作的最佳诱导需要对每个物种的卵进行特定的电击处理，电击时间是一个关键变量，但预处理孵育温度影响发育速度，从而影响最佳的绝对电击时间。有丝分裂间隔(τ0)是正常温度下卵孵化发育率的可靠指标，因此可用于估计染色体操作的最佳时间，对白鲟和铲鼻鲟的有丝分裂间隔进行了估计。

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The Progressive Fish-culturist

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