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Formalin for External Protozoan Parasites 外部原生动物寄生虫的福尔马林
Pub Date : 2011-01-09 DOI: 10.1577/1548-8640(1940)7[1:FFEPP]2.0.CO;2
F. Fish
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引用次数: 6
Induced Diploid Gynogenesis in White Bass 白鲈鱼诱导二倍体雌核发生
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0288:IDGIWB>2.0.CO;2
B. Gomelsky, N. Cherfas, A. Gissis, G. Hulata
Abstract Optimal conditions were determined for induction of meiotic diploid gynogenesis in white bass Morone chrysops. In two series of experiments, ultraviolet light (UV) irradiation doses to inactivate sperm genome and optimal temperature shock to restore diploidy were established. Eggs of white bass were inseminated with sperm from striped bass M. saxatilis diluted (1:30) in saline and irradiated with UV at doses ranging from 50 to 1,200 J/m2. A typical “Hertwig effect” was observed with increasing UV dose. Larvae obtained at doses of 400 J/m2 and greater were abnormally developed (haploid syndrome) and had a haploid chromosome number (n = 24). Heat shocks to cause retention of the second polar body were applied. Heat shocks of 2-min duration at 36°C, 38°C, or 40°C were applied at 2 or 3 min after insemination to white bass eggs inseminated with irradiated (800 J/m2) striped bass sperm. The best results were obtained after application of 36°C heat shock at 3 min after insemination, which induced a sig...
摘要确定了白鲈鱼二倍体雌核分裂诱导的最佳条件。通过两个系列的实验,确定了紫外线照射灭活精子基因组的剂量和恢复二倍体的最佳温度冲击。用盐水稀释(1:30)的萨氏条纹鲈鱼精子对白鲈鱼卵进行受精,并用50 ~ 1200 J/m2剂量的紫外线照射。随着紫外线剂量的增加,观察到典型的“Hertwig效应”。在400 J/m2及以上剂量下获得的幼虫发育异常(单倍体综合征),染色体数目为单倍体(n = 24)。热冲击引起第二极体的保留。在授精后2或3分钟,分别在36°C、38°C或40°C条件下对辐照(800 J/m2)条纹鲈鱼精子受精的白鲈鱼卵施加持续2分钟的热冲击。在人工授精后3 min施加36℃热休克,效果最好。
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引用次数: 15
Growth and Survival of Lake Sturgeon Larvae Fed Formulated Diets 配方饲料对湖鲟幼虫生长和存活的影响
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0293:GASOLS>2.0.CO;2
M. N. DiLauro, W. F. Krise, K. Fynn-Aikins
Abstract We conducted a 60-d experiment to ascertain diet-related differences in survival and growth of larval lake sturgeon Acipenser fulvescens. We offered formulated diets to larval lake sturgeon 2 weeks after first-feeding to find a suitable formulated diet. The five formulated diets were fed to larvae in combination with live brine shrimp Artemia sp., which served as a control. There was no diet effect (P ≤ 0.05) on mean survival, which ranged from 67.4% to 58.3%. Lake sturgeon fed only brine shrimp were significantly greater in mean length (60.4 mm) at 68 d of age than all other treatments, and BioKyowa produced the lowest (44.2 mm) mean length. Mean weight of fish provided brine shrimp (0.74 g) was significantly greater than the weights of those fed the Tunison, Biodiet, or BioKyowa diets. There was no indication that larval lake sturgeon had consumed the formulated feeds; rather, it is likely they may have become imprinted on brine shrimp during the 2 weeks immediately following the onset of exoge...
摘要本研究通过60 d的试验,研究了不同饵料对湖鲟(acpenser fulvescens)幼鱼存活和生长的影响。在湖鲟首次投喂2周后,对湖鲟仔鱼进行配方饲粮试验,以寻找合适的配方饲粮。将配制的5种饲料与活卤对虾(Artemia sp.)配合饲喂,并以其为对照。饲粮对平均存活率无显著影响(P≤0.05),平均存活率为67.4% ~ 58.3%。湖鲟在68日龄时的平均体长(60.4 mm)显著高于其他所有处理,而BioKyowa的平均体长最低(44.2 mm)。提供盐水虾的鱼的平均体重(0.74 g)显著大于饲喂Tunison、Biodiet或BioKyowa日粮的鱼的体重。没有迹象表明湖鲟幼虫食用了配方饲料;相反,它们很可能是在外热开始后的两周内就被印在了盐水虾身上。
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引用次数: 31
Managing Fish Hatchery Phosphorus Discharge through Facility Design and Waste Solids Management: A Field Assessment in Nearshore Oneida Lake, New York 通过设施设计和废物固体管理管理鱼类孵化场的磷排放:纽约近岸奥尼达湖的实地评估
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0263:MFHPDT>2.0.CO;2
E. Mills, Richard T. Colesante
Abstract The discharge of phosphorus from fish hatcheries to receiving waters has often been associated with nuisance algal growth. The objective of this study was to evaluate the effect of phosphorus from a newly constructed public fish hatchery (known as the Oneida Fish Culture Station or OFCS) on Cladophora abundance in nearshore areas of Oneida Lake, New York. Our approach was to monitor total phosphorus (TP) and soluble reactive phosphorus (SRP) above and below the OFCS (on Scriba Creek) and at a reference site (Murray Creek) and to index Cladophora abundance at nearshore areas surrounding both streams from late April to early October 1993–1995. The OFCS has a unique particulate disposal system that involves the settling and subsurface leaching of supernatant to minimize phosphorus discharges. The amount of phosphorus entering Scriba Creek annually from the OFCS was estimated to be less than 38 kg. Although concentrations of TP and SRP tended to be higher downstream of the OFCS, these higher levels w...
从鱼类孵化场到接收水域的磷排放经常与有害藻类的生长有关。本研究的目的是评估新建成的公共孵化场(被称为奥奈达养鱼站或OFCS)的磷对纽约奥奈达湖近岸地区Cladophora丰度的影响。我们的方法是在1993-1995年4月下旬至10月初期间监测OFCS (Scriba Creek)和参考点(Murray Creek)上方和下方的总磷(TP)和可溶性活性磷(SRP),并对两条河流周围近岸地区的Cladophora丰度进行指数测定。OFCS有一个独特的颗粒处理系统,包括沉淀和上清的地下浸出,以尽量减少磷的排放。据估计,每年从OFCS进入斯克里巴溪的磷量不到38公斤。虽然TP和SRP的浓度在OFCS的下游趋于较高,但这些较高的浓度在海洋中是不存在的。
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引用次数: 3
Return Rates of Transported Steel head Smolts with and without a Rest Period before Release 在释放前有和没有休息时间的钢头幼崽的回报率
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0284:RROTSH>2.0.CO;2
J. Tipping
Abstract Postrelease smolt survival of summer steelhead Oncorhynchus mykiss that had been trucked 0.75 h and released was compared with that of smolts that had been trucked and allowed to rest for 24 h before release. Results showed no difference in return rates, suggesting that resting after transport may not improve steelhead survival.
摘要对夏钢头Oncorhynchus mykiss放鱼0.75 h后放鱼的幼崽存活率与放鱼24 h后放鱼的幼崽存活率进行了比较。结果显示回归率没有差异,表明运输后休息可能不会提高虹鳟的存活率。
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引用次数: 4
Renewal of Voluntary Feeding by Wild-Caught Atlantic Sturgeon Juveniles in Captivity 野生大西洋鲟鱼在圈养环境中自愿进食的更新
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0311:ROVFBW>2.0.CO;2
M. N. DiLauro
Abstract A modification of previously documented force-feeding procedures resulted in the renewal of appetite by several wild-caught juvenile Atlantic sturgeon Acipenser oxyrinchus. Four of four fish under controlled laboratory conditions responded positively to this technique within 1 month. These fish exhibited negative mean growth rates of −2.71 g/d before force-feeding and gained an average of 2.61 g/d at 285 d after initiation of force-feeding. Mean lengths, weights, and coefficients of condition (K) also increased after force-feeding. This method may be useful in other situations in which the holding of juveniles or adults is necessary, such as in zoos and public aquaria.
对先前记录的强制喂食程序的修改导致了几种野生捕获的大西洋鲟幼鱼食欲的更新。在受控的实验室条件下,4条鱼中有4条在1个月内对该技术反应积极。这些鱼在强制喂食前的平均生长率为负2.71 g/d,在开始强制喂食后285 d的平均生长率为2.61 g/d。平均体长、体重和条件系数(K)在强行喂食后也有所增加。这种方法在其他需要饲养幼鱼或成年鱼的情况下也很有用,比如在动物园和公共水族馆。
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引用次数: 3
Construction of an Inexpensive Three‐Dimensional Motion Platform Shaker 一种廉价的三维运动平台振动筛的构造
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0319:COAITD>2.0.CO;2
M. Landau
Abstract There are many applications in biology and aquaculture for a shaker, including the suspension of small batches of eggs or algal cultures. Three-dimensional motion platform shakers provide uniformly mixed fluids with little or no foaming, but they are expensive. If only a one-speed device for suspending fluids is needed, one of these shakers can be constructed simply, in less than an hour if all the materials are at hand, for about US$75. Construction centers around a subfractional gear motor, a “lazy susan” turntable swivel with ball-bearings, and a slightly eccentric angled hole that is drilled in a block of wood attached to the lazy susan.
摇床在生物学和水产养殖中有许多应用,包括小批量鸡蛋或藻类培养的悬浮。三维运动平台振动器提供均匀的混合流体,很少或没有泡沫,但它们很昂贵。如果只需要一个单速装置来悬浮液体,一个这样的振动筛可以简单地建造,如果所有的材料都在手边,在不到一个小时的时间内,大约75美元。建筑中心围绕着一个次齿轮马达,一个“懒苏珊”转盘旋转与球轴承,和一个稍微偏心的角度孔,是在一块木头上钻到懒苏珊。
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引用次数: 0
Acute toxicity of ammonia and nitrite to shortnose sturgeon fingerlings 氨和亚硝酸盐对短鼻鲟幼鱼的急性毒性
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0315:ATOAAN>2.0.CO;2
J. J. Isely, J. Tomasso
Abstract The 96-h median-lethal concentration (96h LC50) of total ammonia nitrogen (ammonia-N) to fingerling shortnose sturgeon Acipenser brevirostrum was 149.8 ± 55.20 mg/L (mean ± SD, 17.9 ± 0.62°C, pH = 6.8–7.3). Calculated 96-h LC50 for un-ionized ammonia-N was 0.58 ± 0.213 mg/L. The 96-h LC50 of nitrite nitrogen to shortnose sturgeon fingerlings was 11.3 ± 8.17 mg/L (17.9 ± 0.31°C, <1.0 mg chloride/L, <1.0 mg magnesium/L, 1.8 mg calcium/L, 7.7 mg sodium/L).
摘要总氨氮(氨氮)对短鼻鲟幼鱼96h的中位致死浓度(96h LC50)为149.8±55.20 mg/L(平均±SD, 17.9±0.62℃,pH = 6.8 ~ 7.3)。计算出未电离氨氮的96 h LC50为0.58±0.213 mg/L。亚硝酸盐氮对短吻鲟鱼种的96 h LC50为11.3±8.17 mg/L(17.9±0.31℃,<1.0 mg氯/L, <1.0 mg镁/L, 1.8 mg钙/L, 7.7 mg钠/L)。
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引用次数: 31
Impacts of Trout Culture Effluent on Water Quality and Biotic Communities in Virginia Headwater Streams 鳟鱼养殖废水对弗吉尼亚水源水质和生物群落的影响
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0247:IOTCEO>2.0.CO;2
Jason H. Selong, L. A. Helfrich
Abstract Five Virginia trout farms were selected for examination of effluent impacts on downstream water quality, periphyton production, and composition of macroinvertebrate and fish communities during fall 1994 and summer 1995. Annual trout production and feeding rates varied from 18.5 to 59.5 thousand kilograms and 15.6 to 87.6 thousand kilograms, respectively, and were correlated with discharge. Substrate embeddedness increased significantly (P < 0.07) downstream at two farms, but settleable solid concentrations in effluent were always less than 0.1 mL/L. Total ammonia nitrogen, un-ionized ammonia nitrogen, and nitrite nitrogen levels increased significantly (P < 0.001) downstream but were well below recommended thresholds for lethal exposure for aquatic organisms. Dissolved oxygen levels were significantly reduced (P < 0.001) downstream at postfeeding and predawn hours but were typically greater than 7.0 mg/L. Effluent water temperatures, pH, nitrate nitrogen, and total phosphorus concentrations did n...
在1994年秋季和1995年夏季,选择了五个弗吉尼亚鳟鱼养殖场,研究了污水对下游水质、周围植物产量以及大型无脊椎动物和鱼类群落组成的影响。年鳟鱼产量和摄食率分别在18.5 ~ 5.95万kg和15.6 ~ 8.76万kg之间,并与放水量相关。在两个养殖场下游,基质嵌入率显著增加(P < 0.07),但废水中可沉降固体浓度始终低于0.1 mL/L。总氨氮、未电离氨氮和亚硝酸盐氮水平在下游显著增加(P < 0.001),但远低于水生生物致死暴露的推荐阈值。在喂食后和黎明前,下游溶解氧水平显著降低(P < 0.001),但通常大于7.0 mg/L。出水温度、pH值、硝态氮和总磷浓度均无显著差异。
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引用次数: 58
The Effect of the Absence or Presence of Channel Catfish Males on Induced Ovulation of Channel Catfish Females for Artificial Fertilization with Blue Catfish Sperm 蓝鲶精子人工授精时雄鲶缺失或存在对雌鲶诱导排卵的影响
Pub Date : 1998-09-01 DOI: 10.1577/1548-8640(1998)060<0297:TEOTAO>2.0.CO;2
R. Dunham, Zhanjiang Liu, B. Argue
Abstract Female channel catfish Ictalurus punctatus were induced to spawn with carp pituitary extract either singly paired with channel catfish males in aquaria (males present) or stocked with multiple females in aquaria or tanks (males absent). There was no difference between ovulation rates of females induced to spawn in the presence or absence of channel catfish males (P = 0.57). Females hand-stripped with males absent produced more eggs per kilogram of body weight (4,585) than females with males present (2,942; P = 0.07). When females in the males-present treatment deposited eggs in the aquaria, which allowed observation of the readiness of females, the number of eggs available for stripping was reduced. There was no difference in fertilization rate between treatments when using sperm from blue catfish I. furcatus (P = 0.35). Females that ovulated with males absent produced a similar (P = 0.12) number of viable eggs per kilogram of female body weight as females that ovulated with males present (3,809 ...
摘要采用鲤鱼脑垂体提取物诱导斑点沟鲶雌鱼在水族箱中与雄性沟鲶鱼单配对产卵(有雄性),或在水族箱或水箱中与多雌性沟鲶鱼一起产卵(无雄性)。在雄性鲶鱼存在或不存在的情况下,雌性鲶鱼诱导产卵的排卵率没有差异(P = 0.57)。每公斤体重,手剥而雄鱼缺席的雌鱼产卵量(4,585)比有雄鱼在场的雌鱼多(2,942);P = 0.07)。当雄性在场的雌性在水族箱中产卵时,可以观察到雌性的准备情况,可用于剥离的卵的数量减少。不同处理的受精率差异无统计学意义(P = 0.35)。在没有雄性的情况下排卵的雌性与有雄性的情况下排卵的雌性每公斤体重产生的活卵数量相似(P = 0.12)(3,809…
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引用次数: 17
期刊
The Progressive Fish-culturist
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