Gene Cloning of Xylanase Glycoside Hydrolase Family 11 from Bacillus halodurans CM1 in Escherichia coli DH5α

Abstract

Xylanase is an enzyme that can break down xylan into xylose and xylooligosaccharide that is widely used in industry. Seeing the many applications of this enzyme, researchers conducted many studies on how to increase the productivity and effectiveness of the xylanase enzyme. One of the method that can be used to increase the xylanase enzyme production process is by using recombinant DNA technology such as cloning. Bacillus halodurans CM1 is a local alkalothermophilic bacterium that potential producer for xylanase and other industrial enzymes. This research was conducted to clone the GH11 xylanase coding gene from Bacillus halodurans CM1 using pJET 1.2 / blunt plasmid as vector into Escherichia coli DH5α as cell host and  determine the nucleotide base sequence of the GH11 xylanase coding gene from Bacillus halodurans CM1. The results showed the GH11 xylanase gene from Bacillus halodurans CM1 was successfully cloned in  Esherichia coli DH5α and based on the results of BLAST nucleotides had 99% similarities with that of endo-1,4-beta -xylanhydrolase (xyn11A) from Bacillus halodurans C-125.