Composition Studies on Tobacco XL: Large Scale Fractionation of the Neutrals of Cigarette Smoke Condensate Using Adsorption Chromatography and Solvent Partitioning

Q3 Agricultural and Biological Sciences Beitrage zur Tabakforschung International/ Contributions to Tobacco Research Pub Date : 1969-12-01 DOI:10.2478/cttr-2013-0225

A. P. Swain, J. E. Cooper, R. Stedman, F. Bock

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引用次数: 3

Abstract

Abstract Ten successive 1 kg samples of cigarette smoke condensate were prepared and the neutrals were removed by solvent partitioning of each kg. After removal of a control sample (41.2 %), the remainder of the neutrals were separated by adsorption chromatography on silicic acid followed by partitioning of the eluates between polar and nonpolar solvents, yielding a total of 10 fractions for biological study. A range of recoveries from 94-109 % of the neutrals was obtained in the ten successive runs; the overall average recovery was 102 %. Small amounts of strongly adsorbed material not eluted from the adsorption columns by the technic could be removed in part by other procedures. Using this method, benzo[a]pyrene was concentrated to a high degree in one fraction, thus permitting the detection of biologically active nonpolynuclear agents in the other fractions on bioassay. Exclusion of light during separation did not alter significantly the benzo[a]pyrene levels obtained in the polynuclear-enriched fraction.

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烟草XL的成分研究:用吸附色谱法和溶剂分馏法对卷烟烟气冷凝水中的中性物进行大规模分馏

摘要:连续制备了10个1 kg的卷烟烟气冷凝液样品，并通过每kg的溶剂分配去除中性物质。在除去对照样品(41.2%)后，剩余的中性物质通过硅酸吸附色谱分离，然后在极性和非极性溶剂之间划分洗脱液，产生总共10个馏分用于生物学研究。在连续10次运行中，获得了94% ~ 109%的回收率范围;总体平均回收率为102%。少量未被该技术从吸附柱中洗脱的强吸附物质可以通过其他程序部分去除。使用这种方法，苯并[a]芘在一个馏分中浓缩到很高的程度，从而允许在生物测定中检测其他馏分中的生物活性非多核试剂。在分离过程中排除光并没有显著改变多核富集部分中获得的苯并[a]芘水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Beitrage zur Tabakforschung International/ Contributions to Tobacco Research Agricultural and Biological Sciences-Agronomy and Crop Science

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