Neutralising the testosterone enanthate-induced oxidative stress in rats uterine tissue by propolis and chicory as natural antioxidants

Abstract

The objective of this study was to investigate the effect of eight weeks of propolis and chicory on oxidative stress of uterine tissue in rats consuming testosterone enanthate. Thirty-five female Wistar rats (8 weeks old and weighing 200±12 g) were randomly divided between five groups (n=8) resistance training+ testosterone enanthate (RT+TE), resistance training+ testosterone enanthate+ chicory (RT+TE+CH), resistance training+ testosterone enanthate+ propolis (RT+TE+PR), control (CO), and sham (RT). The protocols were as follows: exercise protocol including climb on a 1-meter ladder with 26 steps five days a week for eight weeks, received a dose of 20 mg/kg of body weight of testosterone weekly for eight weeks, and received a dose of 400 mg/kg proplis and 6 g/kg chicory of body weight all days a week for eight weeks. Superoxide dismutase (SOD), glutathione peroxidase (GPX), and malondialdehyde (MDA) were measured as oxidative stress markers. Testosterone treatment resulted in a significant decrease in the amount of SOD and GPX only in RT+TE group compared to the RT+TE+CH and RT+TE+PR groups (P=0.001). There was no significant difference in mean SOD and GPX levels between RT+TE+CH and RT+TE+PR with CO and RT groups (P>0.05). MDA level was significantly higher in RT+TE group compared to the other groups (P=0.001). This level was also significantly higher in RT+TE+CH group compared to the CO group (P=0.000) and in RT+TE+PR group compared to CO (P=0.000) and RT (P=0.003) groups. Although testosterone enanthate leads to oxidative stress in uterine tissue, propolis and chicory reduced this oxidative stress. It seems that more research can be done on the potential effects of natural antioxidants to neutralise the harmful effects of steroids.