A Comprehensive Study on Asparagus officinalis: Its Antimicrobial, Antioxidant and Phytochemical Characteristics

A. Mehmood, Malaika Ajaz, Waleed Rasool, Lahraseb Khan, Nida Naeem, A. Hassan
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Abstract

The Asparagus plant is considered to be a palatable chemical source against treating infectious diseases and flavorings. Its prevalent distribution is well-known in Asian and sub-Asian regions. Objective: To understand different activities that have been functional in the stem and leaf extracts of Asparagus officinalis including antioxidant and antibacterial activities. Further, phytochemical constituents of asparagus are also discussed. Methods: The antibacterial assay of extracts for the variety of bacteria, indicated a maximum inhibition zone against Enterococcus faecalis (ATCC 29212) (24 mm) followed by Staphylococcus aureus (ATCC 25923) (34 mm), whereas Bacillus subtilis (ATCC 6633) (14 mm) at their respective temperature a minimum inhibition zone after 24 hours and 48 hours of incubation (37 °C for bacteria). Results: As a robust antioxidant reference standard, these antioxidant activities resulted in the stable radical 1-diphenyl-2-picrylhydrazyl (DPPH). It can be reduced to yellow-coloured DPPH-H, reaching 75.81% of the DPPH scavenging impact at its 100% concentration in contrast to ascorbic acid. Various experiments have been carried out, including the Molisch test, Ninhydrin test, Wagner’s test, Alkaline reagent test, Froth test, Ferric reagent test, and Salkowski test for the phytochemical analysis. Conclusion: To sum that up, carbohydrates, saponins, and flavonoids are present in these extracts. These extracts were found to perform satisfactory activities in all tests.
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芦笋抗菌、抗氧化及植物化学特性的综合研究
芦笋植物被认为是一种可口的化学来源,可以治疗传染病和调味。其普遍分布在亚洲和亚亚洲地区是众所周知的。目的:了解芦笋茎叶提取物的抗氧化和抗菌活性。此外,还讨论了芦笋的植物化学成分。方法:对各种细菌进行抑菌试验,结果表明,在不同温度下,对粪肠球菌(ATCC 29212)的最大抑菌区(24 mm)、金黄色葡萄球菌(ATCC 25923)的最大抑菌区(34 mm)、枯草芽孢杆菌(ATCC 6633)的最大抑菌区(14 mm)分别在孵育24小时和48小时(37℃)后达到最小抑菌区。结果:作为抗氧化参比标准,这些抗氧化活性产生稳定的自由基1-二苯基-2-苦味酰肼(DPPH)。它可以还原成黄色的DPPH- h,与抗坏血酸相比,在其100%浓度下达到75.81%的DPPH清除效果。进行了Molisch试验、Ninhydrin试验、Wagner试验、Alkaline reagent试验、Froth试验、Ferric reagent试验、Salkowski试验等植物化学分析实验。结论:综上所述,这些提取物中含有碳水化合物、皂苷和黄酮类化合物。这些提取物在所有试验中都表现出令人满意的活性。
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