Ma Zhidong, Chang Zili, L. Fang, Huo Yanhong, Lin Jianyun, Wu Zhenghua, Zhang Zhongbing
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引用次数: 0
Abstract
Objective
To determine a method for amplification of cytochrome C oxidase subunit I(CO I) gene of Brandt's vole.
Methods
The Brandt's Voles were captured in Abagaqi Xilingol League Inner Mongolia, and DNA was extracted from liver tissue. CO I gene was amplified by nested PCR and sequenced afterwards.
Results
A band of 657 bp and 1 132 bp was amplified by internal and external PCR primers, respectively, which were consistent with expected sizes. A total of 12 segments of Brandt's Vole CO I gene sequences were amplified by PCR and verified by sequencing. The sequence number was KF182196 - KF182207 in GenBank. After gene sequence alignment of the 12 CO I gene sequences, it was found that the similarity was 100%, and no base mutation.
Conclusion
CO I gene of Brandt's Vole could be amplified by nested PCR without pseudo gene.
Key words:
Nested PCR; Brandt's Vole; Cytochrome C oxidase subunit I gene
期刊介绍：
The Chinese Journal of Endemiology covers predominantly endemic diseases threatening health of the people in the areas affected by the diseases including Keshan disease, Kaschin-Beck Disease, iodine deficiency disorders, endemic fluorosis, endemic arsenism, plague, epidemic hemorrhagic fever, brucellosis, parasite diseases and the diseases related to local natural and socioeconomic conditions; and reports researches in the basic science, etiology, epidemiology, clinical practice, control as well as multidisciplinary studies on the diseases.
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