Effect of Cultural Filtrate of Fusarium oxysporum. f. sp. tuberosi in Some Growth Parameters of Ten Potato Varieties (Solanum tuberosum) In vitro

Abstract

Eltinawi, E.A., F. Albiski and J. Faddoul. 2022. Effect of Cultural Filtrate of Fusarium oxysporum. f. sp. tuberosi in Some Growth Parameters of Ten Potato Varieties (Solanum tuberosum) In vitro. Arab Journal of Plant Protection, 40(3): 247- 259. https://doi.org/10.22268/AJPP-40.3.247259 This study aimed to evaluate the effect of biological stress on some morphological growth parameters of ten potato varieties grown in vitro. Biological stress was applied by adding different concentrations of cultural filtrate (CF) of the fungal pathogen Fusarium oxysporum. f. sp. Tuberosum (0, 5 , 10, 15, 25, 30, 40%) to the MS (Murashigi-Skoog) growth medium. The growth parameters measured were: plant height (cm), number of leaves/plant, leaf area (mm²), number of roots/plant, root length (cm), and plant fresh and dry weight (g). The results showed that the potato varieties investigated differed in their response to the biological stress applied. The cluster analysis based on the sum of the relative values of the studied growth parameters, the potato varieties can be clustered in three different groups: The first group included three tolerant varieties to biological stress, Toronto, Barcelona and Suria; the second group included four moderately sensitive varieties to bio-stress, Fabulla, Nectare, Spunta and Ardappel; the third group included three sensitive varieties, 7-Four-7, Farida and Joly. The results obtained indicated that the in vitro screening technology could be used as a fast and efficient way to evaluate genetic variation of tolerance to biological stress in potatoes. Keywords: Biological stress, cultural filtrate (CF), cluster analysis, mycotoxin, potatoes