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Detection of the Enzymes and Toxins Produced by the Pathogenic Fungi that Cause Chias Pollen Disease on Different Date Palm Cultivars in Iraq 伊拉克不同枣椰树品种花粉病病原真菌产酶和毒素的检测
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.065070
Khalaf A. Mohamad, Maadh A. Fahed
Mohamed, Kh.A. and M.A. Alfahd. 2023. Detection of the Enzymes and Toxins Produced by the Pathogenic Fungi that Cause Chias Pollen Disease on Different Date Palm Cultivars in Iraq. Arab Journal of Plant Protection, 41(1): 65-70. https://doi.org/10.22268/AJPP-41.1.065070 This study aimed to determine the exo-enzyme activity of some inflorescence-rot causing fungi that were isolated from different date palm cultivars such as Mauginiella scaettae, Fusarium solani, Fusarium oxysporium and Alternaria radicina and their ability to produce protease and cellulase enzymes on Czapek dox (CD) liquid media supplemented with carboxy methyl cellulose (CMC), as well as their ability to produce Fumonisin B1 toxin using the enzyme linked immunoassay assay (ELISA). The results obtained showed a discrepancy in the amount of enzymes and toxins produced by these pathogens. The fungus F. oxysporum produced the highest cellulase enzyme activity, which amounted to 1.1933 units/ml, whereas Alternaria radicina produced the highest protease enzyme activity which amounted to 50,964 units/ml. The pathogen M. scaettae produced the highest toxin concentration of 38.465 µg/kg, whereas the pathogen Fusarium solani did not produce this toxin. Keywords: Mauginiella. Scaettae, enzymes, fumonisin B1, toxins.
穆罕默德,Kh.A。和M.A. Alfahd. 2023。伊拉克不同枣椰树品种花粉病病原真菌产酶和毒素的检测植物保护学报,41(1):65-70。https://doi.org/10.22268/AJPP-41.1.065070本研究旨在测定从不同枣椰树品种中分离得到的一些致花序腐烂真菌(Mauginiella scaettae、Fusarium solani、Fusarium oxysporium和Alternaria radicina)的外酶活性,以及它们在添加羧甲基纤维素(CMC)的Czapek dox (CD)液体培养基上产生蛋白酶和纤维素酶的能力。以及利用酶联免疫测定法(ELISA)产生伏马菌素B1毒素的能力。所获得的结果表明,这些病原体产生的酶和毒素的数量存在差异。纤维素酶活性最高的真菌为尖孢霉(F. oxysporum),为1.1933 units/ml;蛋白酶活性最高的真菌为radicina Alternaria,为50964 units/ml。病原菌scaettae产生的毒素浓度最高,为38.465µg/kg,而病原菌Fusarium solani不产生该毒素。关键词:Mauginiella。黄芩,酶,伏马菌素B1,毒素。
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引用次数: 0
Control of Spodoptera exigua Which Attacks Pepper Crop in Armenia Using Different Chemical Insecticides 不同化学杀虫剂防治亚美尼亚辣椒夜蛾的研究
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.037039
H.L . Terlemezyan, M. Ghazaryan
Terlemezyan, H.L. and M.H. Ghazaryan. 2023. Control of Spodoptera exigua Which Attacks Pepper Crop in Armenia Using Different Chemical Insecticides. Arab Journal of Plant Protection, 41(1): 37-39. https://doi.org/10.22268/AJPP-41.1.037039 Spodoptera exigua (syn. Caradrina exigua Hübner) is a major pest of pepper in Armavir region, Armenia, and the damage is caused by the larval stage of the insect. During the 2020-2021 growing season, a field experiment was conducted at Khoronk, Armavir region, to control Spodoptera exigua using different chemical insecticides (Eforia, Coragen, Corragen, DecisProfi, Belt, Decis f-Lux, Movento, Ampligo 150, Vertimec, Prokleym, Spintor), in addition to the commonly used insecticide Arrivo, as a control. Results obtained showed that the insecticides Spintor, Belt, Decis f-Lux and Prokleym were the most effective against Spodoptera exigua. Keywords: Spodoptera exigua, pepper, chemical control, Armenia.
Terlemezyan, H.L.和M.H. Ghazaryan, 2023。不同化学杀虫剂防治亚美尼亚辣椒夜蛾的研究植物保护学报,41(1):37-39。https://doi.org/10.22268/AJPP-41.1.037039夜蛾(Spodoptera exigua)是亚美尼亚Armavir地区辣椒的主要害虫,其危害是由幼虫期引起的。在2020-2021年生长季,在Armavir地区Khoronk进行了除常用杀虫剂Arrivo外,使用不同化学杀虫剂(Eforia、Coragen、Corragen、DecisProfi、Belt、Decis f-Lux、Movento、Ampligo 150、Vertimec、Prokleym、Spintor)防治夜蛾的田间试验。结果表明,spindtor、Belt、Decis f-Lux和Prokleym等杀虫剂对夜蛾的防治效果最好。关键词:夜蛾,辣椒,化学防治,亚美尼亚
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引用次数: 1
Biology and Feeding Potential of the Predator, Hippodamia convergens Guér., on Four Aphid Species Under Laboratory Conditions 食肉动物的生物学和摄食潜力。,在实验室条件下的四种蚜虫
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.048053
Ahmed Amin Ahmed Saleh, Hend S. El-Tahawe, Heba Elnagar, Ahmed Shmhi Jabbar
Saleh, A.A.A., H.S. El-Tahawe, H. El-Nagar and A.S. Jabbar. 2023. Biology and Feeding Potential of the Predator, Hippodamia convergens Guér., on Four Aphid Species Under Laboratory Conditions. Arab Journal of Plant Protection, 41(1): 48-53. https://doi.org/10.22268/AJPP-41.1.048053 Suitability of the aphid species Brevicoryne brassicae L., Aphis craccivora Koch, Hyalopterus pruni Geoffroy and Aphis illinoisensis Shimer as preys for the predator Hippodamia convergens Guérin-Méneville (Coleoptera: Coccinellidae) development and reproduction was evaluated under laboratory conditions of 20±2ºC temperature and 70±5% relative humidity. Results obtained revealed that the prey species had significant effect on the development and predation rate of H. convergens immatures. The shortest total larval developmental period (12.82 days) was observed for the beetle fed on A. craccivora, whereas the longest (16.32 days) was recorded for the beetle fed on H. pruni. H. convergens consumed significantly more individuals of H. pruni (278.34) during its larval period than A. craccivora (186.76), B. brassicae (227.87) and A. illinoisensis. (211.94). The lowest immature stages mortality rate was recorded when they were fed on A. craccivora (6.47%), followed by those fed on A. illinoisensis (8.05%), B. brassicae (9.65%) and H. pruni (13.6%). Meanwhile, adult females consumed significantly more individuals of H. pruni (4937.40) than B. brassicae, A. illinoisensis (3693.92, 3432.15) and A. craccivora (2953.5). Highest fecundity (564.00 eggs) was recorded when females of H. convergens were fed on A. craccivora. In addition, beetle adult females and males that fed on H. pruni had significantly longer life span than those fed on the other three aphid species. The investigation provides useful information for the utilization of the H. convergens predator in IPM programs to protect faba bean, cabbage, grapevine and stone fruit trees from aphids as well as other sap sucking pests. Keywords: Hippodamia convergens, Brevicoryne brassicae, Aphis craccivora, Hyalopterus pruni, Aphis illinoisensis, biology, reproduction, feeding capacity.
Saleh, a.a.a., H.S. El-Tahawe, H.S. El-Nagar和A.S. Jabbar. 2023。食肉动物的生物学和摄食潜力。,在实验室条件下的四种蚜虫。植物保护学报,41(1):48-53。https://doi.org/10.22268/AJPP-41.1.048053在温度为20±2℃、相对湿度为70±5%的实验室条件下,研究了芸苔蚜(Brevicoryne brassicae L.)、克拉科蚜(Aphis craccivora Koch)、geoffrohyalopterus pruni Geoffroy和伊利诺伊州蚜(Aphis illisensis Shimer)等蚜虫对瓢虫(鞘翅目:Coccinellidae)的发育和繁殖的适宜性。结果表明,猎物种类对会聚小圆蝽的发育和捕食率有显著影响。幼虫总发育周期最短的为12.82 d,最长的为16.32 d。在幼虫期,会聚小蠊对褐毛小蠊的摄取量(278.34)显著高于裂缝小蠊(186.76)、芸苔小蠊(227.87)和伊利诺伊州小蠊(227.87)。(211.94)。未成熟期死亡率最低的是采食裂腹蠓(6.47%),其次是采食伊利诺伊州蠓(8.05%)、十字花科蠓(9.65%)和普吕尼蠓(13.6%)。雌成虫对褐毛小蠊的摄取量(4937.40)显著高于芸苔小蠊、伊利诺伊州小蠊(3693.92、3432.15)和克拉科小蠊(2953.5)。结果表明,聚敛血蜱雌虫以裂口蠓为食,产卵量最高,为564.00个卵。此外,以pruni为食的成虫雌雄寿命明显长于其他3种蚜虫。本研究为在综合防治中利用会聚小蜂保护蚕豆、白菜、葡萄藤和核果等果树免受蚜虫和其他吸液害虫的侵害提供了有益的信息。关键词:会聚希波达马,芸苔短尾蛾,克拉科蚜,普吕氏透明翅虫,伊利诺伊州蚜,生物学,繁殖,摄食能力
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引用次数: 0
Evaluation of the Efficacy of Commercial Plant Extracts of Palazin and Tondxier Against the Red Flour Beetle Tribolium castaneum (Herbest) Under Laboratory Conditions 在实验室条件下评价帕拉津和通德西耶市售植物提取物对红粉甲虫的防治效果
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.054057
Noor C.H Al-Fatlawy, Sienaa M. Al-Zurfi
Al-Fatlawi, N.C.H. and S. Al-Zurfi. 2023. Evaluation of the Efficacy of Commercial Plant Extracts of Palazin and Tondxier Against the Red Flour Beetle Tribolium castaneum (Herbest) Under Laboratory Conditions. Arab Journal of Plant Protection, 41(1): 54-57. https://doi.org/10.22268/AJPP-41.1.054057 The Effect of Tondxier and Palazin plant extracts on the mortality of the flour beetle T. castaneum (Herb) was investigated. Plant extracts were separately tested under laboratory conditions against the beetles using a topical spray. Beetles were sprayed with plant suspensions at different concentrations (1, 2 and 3 ml/L) for both Tondxier and Palazin. Mortality was recorded daily for 15 days while beetles were kept on wheat flour. Both Tondxier and Palazin had an impact on T. castaneum. Commercial Tondxier was more effective than Palazin at the same concentrations tested. Nevertheless, significant differences in the mortality rate at the end of the experiments between second, and 5 th instar larvae and adults treated with Palazin and Tondxier extracts were observed. Second and 5 th instar larvae were more susceptible, and adults were more resistant to Palazin and Tondxier treatments. This study showed that mortality rate increases with increased concentration and length of exposure times. Results obtained suggested that the two plant extracts of Palazin and Tondxier used in this study can be used as potent insect control agents. Keywords: Palazin, Tondxier, Tribolium castaneum, larvae
Al-Fatlawi, N.C.H.和S. Al-Zurfi。2023. 在实验室条件下评价帕拉津和通德西耶市售植物提取物对红粉甲虫的防治效果。植物保护学报,41(1):54-57。https://doi.org/10.22268/AJPP-41.1.054057研究了通喜尔和帕拉津两种植物提取物对蓖麻粉甲虫死亡率的影响。在实验室条件下,用局部喷雾分别测试了植物提取物对甲虫的作用。将不同浓度的植物悬浮液(1、2和3 ml/L)喷洒在甲虫身上,用于Tondxier和Palazin。每天记录死亡率,持续15天,同时将甲虫放在小麦粉上。Tondxier和Palazin对castaneum均有影响。在相同的测试浓度下,商业Tondxier比Palazin更有效。然而,实验结束时,Palazin和Tondxier提取物处理的2、5龄幼虫和成虫的死亡率有显著差异。2龄和5龄幼虫对Palazin和Tondxier处理更敏感,成虫对Palazin和Tondxier处理更抗性。这项研究表明,死亡率随着暴露浓度的增加和暴露时间的延长而增加。结果表明,本研究使用的两种植物提取物Palazin和Tondxier可作为有效的防虫剂。关键词:Palazin, Tondxier, castaneum,幼虫
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引用次数: 1
First Record of the Eucalyptus Gall Wasp, Ophelimus maskelli (Ashmead) in Nineveh Governorate, Iraq 伊拉克尼尼微省桉树瘿蜂的首次记录
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.012014
Haitham Mohieldin Muhammad Al-Jalal, Wafaa Abid Yahya
Al-Jalal, H.M.M. and W.A. Yahya. 2023. First Record of the Eucalyptus Gall Wasp, Ophelimus maskelli (Ashmead) in Nineveh Governorate, Iraq. Arab Journal of Plant Protection, 41(1): 12-14. https://doi.org/10.22268/AJPP-41.1.012014 Gall wasps ophelimus maskelli were detected for the first time in Nineveh Governorate on the foliage of Eucalyptus tree during June, 2021. Symptoms of infestation were in the form of pus-like swellings on the fresh stems and leaf blade. Leaves of Eucalyptus camaldulensis were heavily infested with the gall wasp, with leaves infestation rate and severity of infestation reached 42.2% and 5.2 galls/cm², respectively. The results showed that the severity of infestation at the basal and middle leaves was higher than the terminal leaves, and the averages reached 5.38, 2.73 and 1.70 galls/cm², respectively. Keywords: Swelling wasp, Eucalyptus, rate of infestation, severity of infestation.
Al-Jalal, H.M.M.和W.A.叶海亚,2023。伊拉克尼尼微省桉树瘿蜂的首次记录。植物保护学报,41(1):12-14。https://doi.org/10.22268/AJPP-41.1.012014于2021年6月在尼尼微省桉树叶片上首次发现瘿蜂。侵染的症状是在新鲜的茎和叶片上出现脓肿。油蓝桉叶片被瘿蜂侵染较多,侵染率和严重程度分别达到42.2%和5.2瘿蜂/cm²。结果表明,黄芪基叶和中叶的侵染严重程度高于顶叶,平均侵染程度分别达到5.38、2.73和1.70高尔/cm²;关键词:肿胀黄蜂,桉树,侵染率,侵染严重程度。
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引用次数: 0
Relative Feeding Potential and Biology of Fall Armyworm, Spodoptera frugiperda Fed on Different Host Plants 不同寄主植物对秋粘虫的相对取食潜力及生物学研究
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.001007
M. Irfan Ullah, M. Arshad, Waqas Ahmed, N. Altaf, Aqsa Arroj, Muhammad Afzal
Ullah, M.I., M. Arshad, W. Ahmed, N. Altaf, A. Arroj and M. Afzal. 2023. Relative Feeding Potential and Biology of Fall Armyworm, Spodoptera frugiperda Fed on Different Host Plants. Arab Journal of Plant Protection, 41(1): 1-7. https://doi.org/10.22268/AJPP-41.1.001007 Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is a polyphagous insect pest causing major losses of corn crop in Pakistan. Biology and feeding potential of S. frugiperda was evaluated on three economically important crops including maize, Zea maize L. (Poaceae); sorghum, Sorghum bicolor L. (Poaceae); and cabbage, Brassica oleracea L. (Cruciferae) under laboratory conditions. The findings of this study showed that relative consumption rate (RCR) of S. frugiperda was 3.61 mg/mg/day, relative growth rate (RGR) was 0.78 mg/mg/day, the efficiency of conversion of ingested food (ECI) was 31.6%, and approximate digestibility (AD) was 56.46% feeding on maize leaves and was higher compared to other host plants. However, RCR (0.687 mg/mg/day), RGR (0.29 mg/mg/day), ECI (17.5%) and AD (33.96%) values were lower when larvae fed on cabbage leaves. Similarly, large larval instars completed their development faster when they fed on maize leaves. The larval mortality rate was also lower (13.3%) when they fed on maize leaves as compared to sorghum (20.0%) and cabbage (36.7%). Overall, it is concluded that the maize crop was the most suitable host plant for S. frugiperda as the growth and development of the insect was higher compared to other host plants in this study. But in the absence of a favored host i.e. maize, it can feed and develop on other hosts such as sorghum and cabbage. Information on the biology and feeding indices of pests could be very useful in developing integrated pest management tactics and improving crop production. Keywords: Spodoptera frugiperda, biology, nutritional indices, insect-plant interaction
乌拉,M. i ., M. arshhad, W. Ahmed, N. Altaf, A. Arroj和M. Afzal。2023。不同寄主植物对秋粘虫的相对取食潜力及生物学研究。植物保护学报,41(1):1-7。https://doi.org/10.22268/AJPP-41.1.001007秋粘虫(FAW), Spodoptera frugiperda (J.E. Smith)(鳞翅目:夜蛾科)是巴基斯坦玉米作物的主要食性害虫。对三种重要经济作物玉米(玉米科)的生物学特性和取食潜力进行了评价;高粱,高粱双色(禾本科);以及实验室条件下的甘蓝(十字花科)。结果表明:以玉米叶片为食的沙蚕的相对摄取率(RCR)为3.61 mg/mg/d,相对生长率(RGR)为0.78 mg/mg/d,食入食物转化效率(ECI)为31.6%,近似消化率(AD)为56.46%,高于其他寄主植物。而摄食甘蓝叶的幼虫的RCR (0.687 mg/mg/d)、RGR (0.29 mg/mg/d)、ECI(17.5%)和AD(33.96%)较低。同样,以玉米叶片为食的大型幼虫完成发育的速度更快。以玉米叶片为食的幼虫死亡率(13.3%)也低于高粱(20.0%)和卷心菜(36.7%)。综上所述,由于玉米作物的生长发育高于其他寄主植物,因此玉米作物是最适合的寄主植物。但在没有玉米等寄主的情况下,它可以以高粱和卷心菜等寄主为食并在其上发育。关于害虫的生物学和摄食指数的信息对制定害虫综合治理策略和提高作物产量非常有用。关键词:夜蛾;生物学;营养指标
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引用次数: 0
Defense Behavior of Honeybee Apis mellifera L. Hives: A Review 蜜蜂的蜂巢防御行为研究进展
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.085092
Moselem Ashour A. W. Al-Etby
Al-Etby, M.A.A.W. 2023. Defense Behavior of Honeybee Apis mellifera L. Hives: A Review. Arab Journal of Plant Protection, 41(1): 85-92. https://doi.org/10.22268/AJPP-41.1.085092 Beekeeping is an important agricultural activity that contributes to increasing agricultural production. Therefore, it has received a lot of attention through the ages. Despite this importance, it is still facing many problems in all countries especially in Iraq. One of the obstacles of honeybee industry development is the aggressive behavior of this beneficial insect. This review examined the complex integration of external stimuli, reactions, and response to olfactory, visual, and mechanical signals. It was concluded that the strength of the colony, its queen health, her production of eggs, and the abundance of food sources, all have a role in determining the aggressiveness of bees. The physiological explanation of this behavior is the secretion of alarming pheromone, isopentyl acetate, which leads to an increase in the level of serotonin and dopamine in the brains of bee workers. Moreover, the location of the olfactory and visual receptors, and the number of sensory cells in the antennae, which varies according to honeybee races, resulted in a complex response of defensive reactions that include guarding, recruiting, alerting, attracting, culminating, biting, stinging, and pursuing. Keywords: Defense behavior, aggressiveness, venom gland, alarm pheromones, honeybees.
艾比,M.A.A.W. 2023。蜜蜂的蜂巢防御行为研究进展。植物保护学报,41(1):85-92。https://doi.org/10.22268/AJPP-41.1.085092养蜂是一项重要的农业活动,有助于提高农业产量。因此,古往今来,它受到了很多关注。尽管具有这种重要性,但它在所有国家,特别是在伊拉克,仍然面临许多问题。蜜蜂产业发展的障碍之一是这种益虫的攻击行为。这篇综述研究了外部刺激、反应和对嗅觉、视觉和机械信号的反应的复杂整合。结论是,蜂群的力量、蜂王的健康状况、产卵量和食物来源的丰富程度,都是决定蜜蜂攻击性的因素。对这种行为的生理解释是,蜜蜂分泌了一种令人警觉的信息素——醋酸异戊酯,它会导致工蜂大脑中血清素和多巴胺水平的增加。此外,嗅觉和视觉感受器的位置,以及触角中感觉细胞的数量,根据蜜蜂的种族而不同,导致了复杂的防御反应,包括守卫、招募、警报、吸引、高潮、咬、刺和追击。关键词:防御行为,攻击性,毒腺,报警信息素,蜜蜂。
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引用次数: 0
A Study on the Toxicity and Sublethal Concentrations of Three Insecticides on the Population Dynamics of Green Lacewing Chrysoperla carnea Stephens 三种杀虫剂对绿草金蝶种群动态的毒性和亚致死浓度研究
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.028036
Ayad Alsendi, Ali Abdulhusien Kareem, ,. M. R. Havasi, G. Golmohammadi
Alsendi, A., A.A. Kareem, M. Havasi and Gh. Golmohammadi. 2023. A Study on the Toxicity and Sublethal Concentrations of Three Insecticides on the Population Dynamics of Green Lacewing Chrysoperla carnea Stephens. Arab Journal of Plant Protection, 41(1): 28-36. https://doi.org/10.22268/AJPP-41.1.028036 The study was carried out to evaluate toxicity and sublethal effects of lufenuron, novaluron, and lambda-cyhalothrin on natural enemies such as Chrysoperla carnea. All sub-lethal concentrations caused a significant decrease in oviposition period, total fecundity, longevity, and total life span of C. carnea compared to the control. Maximum and minimum mean lifespan of C. carnea individuals were observed in the untreated group and LC30 concentration of novaluron. Total fecundity varied from 153.47 offspring/individual in treatment with LC30 novaluron to 300.12 offspring/individual in the control treatment. The highest value for intrinsic and finite rate of increase (r, λ) was obtained by the concentration of lambda-cyhalothrin. The net reproduction rate (R0) reached its lowest level in novaluron treatment. As a result, the effects of sublethal concentrations of lufenuron, novaluron, and lambda-cyhalothrin on C. carnea were assessed and discussed to design improved integrated management programs. Keywords: Integrated pest management, side effects, aphidophagous predators, natural enemies.
Alsendi, A., A.A. Kareem, M. Havasi和Gh。Golmohammadi》2023。三种杀虫剂对绿草金蝶种群动态的毒性和亚致死浓度研究。植物保护学报,41(1):28-36。https://doi.org/10.22268/AJPP-41.1.028036本研究旨在评价氟虫腈、新伐虫腈和高效氯氟氰菊酯对黄蝶等天敌的毒性和亚致死效应。与对照相比,各亚致死浓度均显著降低了蠋蝽的产卵期、总繁殖力、寿命和总寿命。观察未处理组的最大、最小平均寿命和新伐脲LC30浓度。总繁殖力从LC30处理的153.47个后代/个体到对照处理的300.12个后代/个体不等。λ -氯氟氰菊酯的浓度可使本征和有限增率(r, λ)达到最大值。净繁殖率(R0)在新伐隆处理下达到最低水平。因此,评估和讨论了亚致死浓度的氟虫腈、新伐螨腈和高效氯氟氰菊酯对蠋蝽的影响,以设计改进的综合管理方案。关键词:害虫综合治理;毒副作用;食蚜性天敌;
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引用次数: 1
Survey of Citrus Nematode Tylenchulus semipenetrans Causing Citrus Slow Decline in Karbala Province of Iraq 造成伊拉克卡尔巴拉省柑橘缓慢衰退的柑橘线虫半穿透线虫调查
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.008011
Wurood J.A. Jabbar, Estabraq M. Abedulridah
Jabbar, W.J.A. and E.M. Abedulridah. 2023. Survey of Citrus Nematode Tylenchulus semipenetrans Causing Citrus Slow Decline in Karbala Province of Iraq. Arab Journal of Plant Protection, 41(1): 8-11. https://doi.org/10.22268/AJPP-41.1.008011 The study aimed to determine the rate and severity of infection with citrus nematodes associated with the citrus slow decline in orchards and nurseries in different regions of Karbala governorate, including Aoun, Hay Al-Amel, Al-Haidariat, Al-Hurr Al-Saghir, Al-Bubiyat, AlHussainiya, and Al-Hindiya. The nematodes associated with citrus slow decline were isolated and phenotypically diagnosed based on microscopic examination of the infected stained roots, and juveniles in the soil based on the phenotypic characteristics of the citrus nematodes. Infestation rate in citrus orchards was assessed, and the severity of infestation was determined based on the number of females in 2 gm roots and the number of juveniles in 100 gm of the rhizospheric soil surrounding the roots. The causes of infection dissemination and the factors that contribute to the increase of infection in the severely affected orchards were investigated. The results of the field survey showed the presence of citrus nematodes infestation in all orchards and in all regions. The incidence and severity of citrus nematode infection differed between the different sites. It was found that the severity of infection was significantly higher with older trees compared to newly established orchards with young plants. The results of the questionnaire accompanying the field survey showed that most citrus orchards were not subject to nematode control practices. In addition, most farmers were not aware of the importance of plant parasitic nematodes and the damage they can cause, especially citrus nematodes. Keywords: Citrus nematode, Tylenchulus semipenetrans, survey, Iraq
贾巴尔,W.J.A.和E.M.阿比杜里达,2023。造成伊拉克卡尔巴拉省柑橘缓慢衰退的柑橘线虫半穿透线虫调查。植物保护学报,41(1):8-11。https://doi.org/10.22268/AJPP-41.1.008011该研究旨在确定卡尔巴拉省不同地区(包括Aoun、Hay Al-Amel、Al-Haidariat、Al-Hurr Al-Saghir、Al-Bubiyat、AlHussainiya和Al-Hindiya)柑橘果园和苗圃中柑橘缓慢衰退与柑橘线虫感染的比率和严重程度。对与柑橘慢衰相关的线虫进行了分离和表型诊断,根据侵染后根系的显微检查进行了表型诊断,根据柑橘线虫的表型特征对土壤中的幼线虫进行了表型诊断。以2 gm根际土壤中雌虫数和100 gm根际土壤中幼虫数为指标,对柑桔果园的侵染率进行了评价,并确定了侵染严重程度。研究了侵染严重的果园侵染的传播原因和侵染增加的因素。现场调查结果表明,柑橘线虫在所有果园和所有地区都存在。柑桔线虫感染的发生率和严重程度在不同地点存在差异。结果表明,老果树的侵染严重程度明显高于新果树的侵染严重程度。随实地调查而来的问卷调查结果显示,大多数柑橘果园没有采取线虫防治措施。此外,大多数农民没有意识到植物寄生线虫的重要性及其可能造成的危害,特别是柑橘线虫。关键词:柑桔线虫,半透线虫,调查,伊拉克
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引用次数: 0
High-Throughput Sequencing and Bioinformatic Analysis Reveal Presence of the Endogenous Pararetrovirus Tobacco vein clearing virus Genome in the Tomato (Solanum lycopersicum) Host Genome 高通量测序和生物信息学分析揭示了内源性烟草清脉副逆转录病毒基因组在番茄(Solanum lycopersicum)宿主基因组中的存在
Pub Date : 2023-03-01 DOI: 10.22268/ajpp-41.1.077084
Mahmood Othman Abass, Adnan A. Lahuf
Abass, M.O. and A.A. Lahuf. 2023. High-Throughput Sequencing and Bioinformatic Analysis Reveal Presence of the Endogenous Pararetrovirus Tobacco vein clearing virus Genome in the Tomato (Solanum lycopersicum) Host Genome. Arab Journal of Plant Protection, 41(1): 77-84. https://doi.org/10.22268/AJPP-41.1.077084 Endogenous pararetroviral sequences (EPRVs) are repetitive sequences that have been discovered mostly in the Kingdom Plantae, particularly in various species of the family Solanaceae. In this study, the draft genome of an endogenous retrovirus identified by next generation sequencing (NGS), was found integrated in the genome of Solanum lycopersicum. Results of the homology alignment revealed that the virus identified was Tobacco vein clearing virus (TVCV), is a member of the genus Solendovirus, family Caulimoviridae. It consists of a double-stranded DNA genome of 7,760 nucleotides in length. Additionally, it has four open reading frames (ORFs), which encodes Solendovirus typical conserved domains that comprise the putative coat protein (ORF1), putative cell-to-cell movement protein (ORF2), the polyprotein (ORF3), which comprises the aspartic protease, reverse transcriptase and RNase H, as well as the putative Trans-activator factor (ORF4). Sequence alignment analysis revealed that the Iraqi TVCV had 81.60% sequence identity to the INSDC Tobacco vein clearing virus (AF190123.1), that was only reported to be integrated in the genome of some species of Nicotiana spp. However, in the current study, TVCV genome was identified associated with genome of S. lycopersicum. This new fact was further verified through BLASTn analysis that confirmed the presence of TVCV genome associated with the genome of several cultivated and wild S. lycopersicum worldwide. In conclusion, the TVCV is the first EPRVs of Solendovirus members discovered in the S. lycopersicum identified from Iraq. Keywords: Tobacco vein clearing virus; Solanum lycopersicum genome, next generation sequencing, NGS
Abass,作案手法和A.A. Lahuf, 2023。高通量测序和生物信息学分析揭示了内源性烟草清脉副逆转录病毒基因组在番茄(Solanum lycopersicum)宿主基因组中的存在植物保护学报,41(1):77-84。https://doi.org/10.22268/AJPP-41.1.077084内源性副逆转录病毒序列(Endogenous pararetroviral sequences, EPRVs)是一种重复序列,主要在植物界发现,特别是在茄科的各种物种中。在本研究中,通过下一代测序(NGS)鉴定的一种内源性逆转录病毒的草图基因组被整合到茄(Solanum lycopersicum)的基因组中。同源比对结果表明,该病毒为烟叶清静脉病毒(TVCV),属烟叶清静脉病毒科索兰病毒属。它由长度为7760个核苷酸的双链DNA基因组组成。此外,它有四个开放阅读框(orf),编码索兰病毒典型的保守结构域,包括假定的外壳蛋白(ORF1)、假定的细胞间运动蛋白(ORF2)、多蛋白(ORF3),包括天冬氨酸蛋白酶、逆转录酶和RNase H,以及假定的反式激活因子(ORF4)。序列比对分析表明,伊拉克TVCV与INSDC烟草清静脉病毒(AF190123.1)序列同源性为81.60%,该病毒仅在烟草属的部分物种基因组中被整合,而本研究发现该病毒基因组与番茄葡萄球菌(S. lycopersicum)基因组存在关联。BLASTn分析进一步证实了这一新的事实,该分析证实了TVCV基因组与世界各地几种栽培和野生番茄葡萄球菌基因组的关联。总之,该TVCV是在伊拉克番茄葡萄球菌中发现的首个含索兰病毒成员的eprv。关键词:烟草清静脉病毒;番茄茄基因组,下一代测序,NGS
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引用次数: 2
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Arab Journal for Plant Protection
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