Spectrophotometric Determination of Metoclopramide Hydrochloride in Pharmaceutical Preparations Via Schiff ’s Base Reaction

Abstract

A method for determining metoclopramide (MCPD) as pure and in formulations has been suggested. The proposed method is characterized by simplicity, speed, and sensitivity. It is a spectrophotometric method based on the condensation interaction between the primary aromatic amine group in metoclopramide and the carbonyl group of aromatic aldehyde in the chromogenic reagent 2-hydroxy-1-naphthaldehyde (2HND) in an acidic medium of H 2 SO 4 and absolute ethanol as the optimal medium. The Schiff base product of the condensation reaction is a yellow-colored product, which exhibits the highest absorption intensity at the wavelength of 447 nm. The proposed method is obeyed to Beer's law in the concentration range of 2 to 20 µg/ml, with a determination coefficient of 0.9960, molar absorption of 1.15 x 10 4 l/ mol.cm, while Sandell's sensitivity index has a significance of 0.0306 µg/ cm 2 .The method was estimated statistically by calculating the percentage of relative error (RE%) can express the accuracy and the percentage of relative standard deviation (RSD%) can express precession. The method has been successfully applied in the determination of metoclopramide in pharmaceutical preparations in the form of tablets and injections.