A Lab-on-a-Chip For The Extraction Of Bacterial Nucleic Acids From Whole Human Blood

M. Hügle, Lara S. Siegel, O. Behrmann, F. Hufert, G. Dame, G. Urban
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引用次数: 1

Abstract

We present a lab-on-a-chip for the extraction of bacterial nucleic acids from whole human blood (WHB). Compared to classical methods, with extraction times between hours and days, the presented chip can lyse the bacteria suspended in WHB and extract their nucleic acids within three minutes. This is achieved by directly coupling thermal lysis to gel-electrophoresis on a single microfluidic platform.
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从人全血中提取细菌核酸的芯片实验室
我们提出了一种从人全血(WHB)中提取细菌核酸的芯片实验室。与传统的提取方法相比,该芯片可以在3分钟内裂解悬浮在WHB中的细菌并提取其核酸,提取时间从数小时到数天不等。这是通过在单个微流控平台上直接耦合热裂解和凝胶电泳来实现的。
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