Nanosized zinc, epigenetic changes and its relationship with DMBA induced breast cancer in rats

IF 3.6 3区 化学 Q2 CHEMISTRY, ANALYTICAL Reviews in Analytical Chemistry Pub Date : 2020-01-01 DOI:10.1515/revac-2020-0104
B. Bobrowska-Korczak, K. Domanska, D. Skrajnowska, R. Wrzesień, J. Giebułtowicz, W. Bielecki, Rafał Wyrębiak, U. Piotrowska, M. Sobczak, J. Kałużna-Czaplińska
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引用次数: 2

Abstract

Abstract The aim of the research was to compare the impact of nano- and micro-sized-zinc on the kinetics of changes in the level of 3-methyladenine, 7-methylguanine, 7-methylguanosine, O-methylguanosine, 1-methyladenosine, N6-methyl-2’-deoxyguanosine in urine of rats with breast cancer. Female Sprague-Dawley rats divided into 3 groups were used in the study. Animals were fed only a control diet or diets supplemented with the nano and micro-sized zinc particles. To induce the mammary cancer (adenocarcinoma), rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA). Modified nucleosides were determined by a validated high performance liquid chromatography coupled to mass spectrometry method. In the first stage of investigations a synergistic activity of nanosized Zn with DMBA on the growth of the neoplastic process was found. During that time a statistically significant increase in the levels of all six examined markers in the rats’ urine was observed. However, as the experiment continued, the supplementation with nanosized zinc caused inhibition of tumour growth, being followed by regression and remission of tumours, as well as, a statistically significant systematic reduction of the levels of methyl derivatives in the urine. Biopsy images indicated grade 1 tumours with multiple inflammatory infiltrates in the group treated with zinc nanoparticles, whereas, in the other groups, moderately-differentiated grade 2 adenocarcinoma was identified. It was found that the biological activity of zinc depends on the size of applied particles, as the treatment with zinc microparticles has not had much effect on cancer progression.
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纳米锌、表观遗传变化及其与DMBA诱导大鼠乳腺癌的关系
摘要本研究旨在比较纳米和微锌对乳腺癌大鼠尿液中3-甲基腺嘌呤、7-甲基鸟嘌呤、7-甲基鸟嘌呤、o-甲基鸟嘌呤、1-甲基腺嘌呤、n6 -甲基-2′-脱氧鸟嘌呤水平变化动力学的影响。雌性Sprague-Dawley大鼠分为3组。动物只被喂食对照饲料或添加了纳米和微型锌颗粒的饲料。采用7,12-二甲基苯[a]蒽(DMBA)诱导大鼠乳腺癌(腺癌)。改性核苷采用高效液相色谱-质谱联用法测定。在研究的第一阶段,发现纳米锌与DMBA在肿瘤生长过程中的协同作用。在此期间,观察到大鼠尿液中所有六种检测标记物的水平在统计学上显著增加。然而,随着实验的继续,补充纳米锌引起肿瘤生长的抑制,随后肿瘤的消退和缓解,以及尿液中甲基衍生物水平的统计显着系统性降低。活检图像显示,在锌纳米颗粒治疗组中,1级肿瘤伴多发炎症浸润,而在其他组中,鉴定为中度分化的2级腺癌。研究发现,锌的生物活性取决于所施用颗粒的大小,因为锌微粒的治疗对癌症的进展没有太大的影响。
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来源期刊
Reviews in Analytical Chemistry
Reviews in Analytical Chemistry 化学-分析化学
CiteScore
7.50
自引率
0.00%
发文量
15
审稿时长
>12 weeks
期刊介绍: Reviews in Analytical Chemistry publishes authoritative reviews by leading experts in the dynamic field of chemical analysis. The subjects can encompass all branches of modern analytical chemistry such as spectroscopy, chromatography, mass spectrometry, electrochemistry and trace analysis and their applications to areas such as environmental control, pharmaceutical industry, automation and other relevant areas. Review articles bring the expert up to date in a concise manner and provide researchers an overview of new techniques and methods.
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