Vibrio El Torのアミノ酸要求について

Abstract

It was reported by Gott; and her coworkers (1959), that Vibrio El Tor strain Denken grow in a semisynthetic media consisting of Casamino acids, salts, glucose, vitamins and purine. The author confirmed that Casamino acids in this composition could be substituted with 18 pure amino acids mixture, and tried to simplify the amino acids composition by the elimination test. This strain could grow in a medium containing 8 amino acids, Methionine, Asparatic acid, Leucine, Phenylalanine, Isoleucine, Lysine, Arginine and Glutamic acid. Stain Denken showed moderate growth in this medium, a little less than in 1% Casamino acids medium or 18 amino acids mixture, and in this media 5 serial transfer was possible. Hemolysine production in this medium was approximately on the same level as in 1% Casamino acids medium.