Molecular analysis of heat shock protein with different stress and lipase production from the gut associated microorganisms of Bombyx mori. L

Abstract

The silkworm, Bombyx mori L (Lepidotera: Bombycidae), as a model for Lepidoptera, is an agriculturally important insect for silk production. It is very heat sensitive organism. It mainly feeds on mulberry leaves, although tricuspid cudrania leaves can also act as the forage of the larvae. In the present study to isolate the microorganism from mid gut of silk worms. Screening the lipase producing bacteria in Rhodamine B agar. Characterize the efficient lipase producing isolate by morphological and biochemical characterization methods. To study the growth curve assay for lipase producing bacteria. To perform lipase assay using olive oil substrate, the factors influencing lipase enzyme production. To estimate the weight of protein quantitatively by Lowry’s et a l method. The role of heat shock protein with thermal and UV stress and the protein profiling by SDS-PAGE. Molecular characterization of the isolate by 16Sr RNA sequencing.