Micropropagation of Bambusa oldhamii f. variegata by tissue culture

Abstract

Bambusa oldhamii f. variegata is a form of Bambusa oldhamii，having good ornamental and economic value. This aim is to establish a tissue culture system for its commercial propagation. Multiplication of B. oldhamii f. variegata using different plant growth regulators and concentrations was studied with the lateral buds as the explant and three shoots per cluster. The results showed that 0.1 mgL-1 thidiazuron（TDZ） was best with the proliferation rate up to 3.60 when TDZ（0.000 1，0.001 0，0.010 0，0.100 0， 1.000 0 mgL-1），6-benzylaminopurine（BA）（1，3，5，10 mgL-1）、2-isopentenyladenine （2ip） （1，3， 5，10 mgL-1） and kinetin （KT）（1，3，5，10 mgL-1） were added respectively to determine suitable concentration for tissue culture，but the highest proliferation rate 4.63 was obtained with 3.00 mgL-1 BA + 0.01 mgL-1 TDZ when the combination of BA（0.001，0.010，0.100，1.000，3.000 mgL-1），TDZ（0.000 1， 0.001 0，0.010 0，0.100 0 mgL-1），and also good growing. Three shoots per cluster was treated only with 50.00 mgL-1 indole-3-butyric acid（IBA） for 1 day，then the explants were transferred to MS（Murashige and Skoog） basic medium，After 30 days the rooting rate was up to 55 percent，and developed well. The survival rate of transplanted potted plants was above 85 percent.［Ch，1 fig. 4 tab. 13 ref.］