Specificity of polyclonal antibody-based magnetostrictive biosensors

Jiajia Hu, R. Guntupalli, B. Chin, Jing Hu
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Abstract

Magnetoelastic (ME) biosensors were developed by immobilizing polyclonal antibody using Langmuir-Blodgett (LB) technique onto a magnetostrictive platform, which can offer wireless and remote detecting, a unique advantage over conventional sensor platforms. Due to the magnetoelastic nature of the amorphous magnetostrictive alloy, the sensor exhibits a physical resonance when it undergoes a time-varying magnetic field, a shift in resonance frequency occurs when its mass changes due to bacteria binding, and the bound bacterial cells were visibly confirmed by scanning electron microscopy (SEM) micrographs, then the density of bacteria bound on the biosensor was calculated. The specificity to detect different kinds of bacteria, including Salmonella species and non- Salmonella species was studied. The results show that the prepared polyclonal antibodybased biosensor shows excellent specificity to detect bacteria of Salmonella species, and especially prefers to bind Salmonella typhimurium, almost zero affinity for non- Salmonella species of Listeria monocytogenes.
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基于多克隆抗体的磁致伸缩生物传感器的特异性研究
采用Langmuir-Blodgett (LB)技术将多克隆抗体固定在磁致伸缩平台上,开发了磁弹性(ME)生物传感器,该传感器可以提供无线和远程检测,这是传统传感器平台的独特优势。由于非晶态磁致伸缩合金的磁弹性特性,传感器在时变磁场作用下产生物理共振,细菌结合导致传感器质量发生变化时,传感器的共振频率发生偏移,通过扫描电镜(SEM)可以明显地确认结合的细菌细胞,进而计算结合在传感器上的细菌密度。对沙门氏菌和非沙门氏菌的特异性进行了研究。结果表明,所制备的多克隆抗体生物传感器对沙门氏菌具有良好的特异性,尤其倾向于结合鼠伤寒沙门菌,对非沙门菌的单核增生李斯特菌几乎为零亲和力。
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