Effect of pH and Different Fermentation Time Intervals on the Production of Single Cell Proteins (SCPs) from Potato Peels

Hira Nadeem
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Abstract

Dried cells of microorganisms such as fungi, algae, and bacteria, known as Single-Cell Proteins (SCPs), are utilized as a source of protein supplements in animal feed or human food. These SCPs can be produced through the use of low-cost feedstocks and waste materials as sources of carbon and energy, which can be converted into biomass and concentrated proteins. Objective: To optimize the yield and growth of dry cell biomass through the manipulation of fermentation conditions. Methods: A batch fermentation process was used to produce dry cell biomass from a microorganism. Different pH values, fermentation times, and reactor configurations were tested, and the resulting biomass was analyzed for its protein content. Results: The maximum yield of dry cell biomass was achieved at pH 4.5, with a yield of 1.951 g/100 ml. The maximum dry biomass was achieved after 72 hours of fermentation, with a yield of 2.824 g/100 ml. The maximum yield of dry biomass was achieved with an Airlift fermenter at an aeration rate of 1.0 vvm and a temperature of 35°C for 72 hours, resulting in a yield of 5.452 g/L. The protein content of the dried cell biomass was found to be in the range of 45-55%. Conclusions: This study demonstrates that the yield and growth of dry cell biomass can be optimized by controlling the fermentation conditions, specifically pH, fermentation time, and reactor configuration. These findings may have implications for the industrial-scale production of dry cell biomass, as they offer insight into how to maximize yield and protein content.
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pH和不同发酵时间间隔对马铃薯皮单细胞蛋白生产的影响
真菌、藻类和细菌等微生物的干燥细胞被称为单细胞蛋白(SCPs),被用作动物饲料或人类食品中的蛋白质补充来源。这些scp可以通过使用低成本的原料和废料作为碳和能源来源来生产,这些碳和能源可以转化为生物质和浓缩蛋白质。目的:通过调节发酵条件,优化干细胞生物量的产量和生长。方法:采用间歇式发酵工艺,以微生物为原料生产干电池生物质。测试了不同的pH值、发酵时间和反应器配置,并分析了所得生物量的蛋白质含量。结果:在pH为4.5的条件下,干生物质产率达到最大值,产率为1.951 g/100 ml,发酵72 h后,干生物质产率达到最大值,产率为2.824 g/100 ml。在曝气速率为1.0 vvm,温度为35℃,发酵72 h时,干生物质产率达到最大值,产率为5.452 g/L。干燥后的细胞生物量蛋白质含量在45-55%之间。结论:本研究表明,通过控制发酵条件,特别是pH、发酵时间和反应器配置,可以优化干电池生物量的产量和生长。这些发现可能会对干电池生物质的工业规模生产产生影响,因为它们为如何最大限度地提高产量和蛋白质含量提供了见解。
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