Effects of abiotic stress on the expression of Hsp70 genes in Sogatella furcifera (Horváth).

Abstract

Sogatella furcifera (Horváth), a prominent rice pest in Asia, is a typical R-strategic and highly adaptable insect. Heat shock proteins (Hsps) are highly conserved molecular chaperones regulating responses to various abiotic stresses; however, limited information is available regarding their role in responding to abiotic stress in S. furcifera. This study aimed to investigate the effect of abiotic stresses on the expression of Hsp70 genes in the S. furcifera. Five Hsp70 genes were isolated from S. furcifera, and the expression patterns at different developmental stages and temperatures, upon treatment with different insecticides and ultraviolet A (UV-A) stress, were analyzed. Hsp70 genes were expressed at different developmental stages. Hsp70-2, Hsp70-5, and Hsp70-6 were significantly upregulated upon heat shock at 40 °C for 30 min. Hsp70-3 and Hsp70-4 were significantly upregulated upon heat shock at 30 °C for 30 min. Under UV-A stress, Hsp70-3, Hsp70-4, Hsp70-5, and Hsp70-6 were significantly upregulated. Conversely, Hsp70-2 was significantly downregulated under UV-A stress. The five Hsp70 genes were significantly downregulated in 3rd-instar nymphs on exposure to thiamethoxam, buprofezin, and avermectin at LC₁₀ and LC₂₅ concentrations. Hence, Hsp70 genes significantly contribute to the tolerance of S. furcifera to temperature and UV-A stress; however, they are not involved in the response to insecticides.