The Effect of different Concentrations of Silver Nanoparticles on Enzyme Activity and Liver Tissue of Adult Male Wistar Rats in-vivo Condition

Abstract

Nanotechnology as a branch of science which is related to nano materials helps in overcoming the limitations of size and can change the outlook of the world regarding science. Silver nano particles, due to their strong antimicrobial properties, are widely applicable in different industries. So, research on nano silver properties and its harmful effects on human and environment is critical. The aim of the present study is testing the harmful properties of Silver nano particles, in the size 18 to 32 nm, on enzymes and tissues of liver in Wistar rats. At first 50 adult male Wistar rats were picked and concentrations 5, 10, 20 and 40 ppm of Silver nano particle were tested on AST, ALT, ALP and GGT enzymes. Results showed that only 40 ppm of silver nano particle possesses a meaningful effect on ALT enzyme, and none of the other concentrations has meaningful effect on AST, ALP and GGT. Moreover, histopathology test from liver tissue revealed no damage in liver cells. As nano silver shows harmful effects on a broad range of cells, predetermined dosages of nano silver in limited amounts are suggested.