DEVELOPMENT OF THE METHOD FOR DETERMINING THE CONTENT OF THE SYNTHETIC DYE DIAMOND BLUE FCF IN THE SORPTION MATERIAL BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD

Abstract

Introduction. To date, the use of unmanned aerial vehicles (UAVs) in Ukraine for the agricultural lands treatment is a new promising technology that requires detailed study and development of approaches to risk assessment and hygienic regulation. Development of a method that will allow measuring the content of diamond blue FCF in the sorption material (filter paper) is relevant. The aim of the study – development of the method for determining the content of the synthetic dye diamond blue FCF in the sorption material by the high-performance liquid chromatography method. Research Methods. The following materials were used: laboratory analytical balance Radwag® AS220.R2, rotary evaporator, cartridge Strata™ C18-e (55 μm, 70 °С) 500 mg/6 ml, f. Phenomenex, steel chromatographic column 150/4.6 Microsorb 100-5 C18, pre-column chromatographic 4/3 Microsorb 100-5 C18, liquid chromatograph “Shimadzu” with a diode array detector, Diamond blue FCF, standard, 87.7, paper filters de-ashed “red ribbon”. Results and Discussion. At the first stage of the research, samples were taken and prepared. For analysis, 2 parallel samples were taken. The next stage was the preparation of the sample for introduction into the chromatograph. The third stage was chromatography performing. At the final stage, the diamond blue FCF peak areas were determined and calculated on the chromatograms. The indicated method of determining the content of diamond blue FCF in the sorption material (filter paper) includes extraction of the dye from the sorption material (filter paper) with distilled water; solid-phase extraction and quantification of diamond blue FCF by reversed-phase HPLC with SF detection. This method differs from the known ones in that it makes it possible to determine the investigated dye in the sorption material. Conclusion. The proposed method of containing diamond blue FCF in the sorption material (filter paper) will allow to evaluate the effectiveness and safety of the use of various models of UAVs in combination with various pesticide preparations when using different agrotechnical characteristics at the stage of pre-registration trials and scientific research.