Fluorescence imaging of Er3+/Yb3+ codoped tungsten tellurite glasses with silver nanoparticles

Abstract

The Er3+ - Yb 3+ doped TeO2 -WO3- Li2O (TWLEY) ternary doped with and without Ag nanoparticles (NPs) glasses have been synthesized by melt quenching technique. Surface Plasmon resonance bands in the glasses are measured and verified in the range 535-550 nm and 640-650 nm under 488nm laser excitation by lambda scanning technique of the confocal fluorescence microscope. Fluorescence Intensity map of TWLEY and TWLEY03A are observed at 545nm. The Fluorescence Lifetime Imaging Microscopy (FLIM) studies has been conducted for the imaging and lifetime measurement using pulsed laser diode of 405 nm, 6ns,40 MHz of PicoQuant and result are analyzed by decay and lifetime histograms. This work shows that Plasmon enhances photoluminescence of silver and rare-earth doped glasses may be utilized for biomedical imaging.The Er3+ - Yb 3+ doped TeO2 -WO3- Li2O (TWLEY) ternary doped with and without Ag nanoparticles (NPs) glasses have been synthesized by melt quenching technique. Surface Plasmon resonance bands in the glasses are measured and verified in the range 535-550 nm and 640-650 nm under 488nm laser excitation by lambda scanning technique of the confocal fluorescence microscope. Fluorescence Intensity map of TWLEY and TWLEY03A are observed at 545nm. The Fluorescence Lifetime Imaging Microscopy (FLIM) studies has been conducted for the imaging and lifetime measurement using pulsed laser diode of 405 nm, 6ns,40 MHz of PicoQuant and result are analyzed by decay and lifetime histograms. This work shows that Plasmon enhances photoluminescence of silver and rare-earth doped glasses may be utilized for biomedical imaging.