Antibacterial Properties and Mechanism of Gold Nanoparticles Obtained from Pergularia Daemia Leaf Extract

Abstract

The development of environmental dealings for the green synthesis of metal nanoparticle using plant mediated process is evolving an important branch of nano biotechnology. It offers a controlled synthesis of metal nanoparticles with welldefined shape, size, composition and morphology. The present study reveals the green synthesis of gold nanoparticle that is non-toxic, novel and eco-friendly. This synthesis approach is cost effective, eco-friendly and promising method for generation of new materials which can be used for many potential applications in field of science and medical. The colour change in UV-visible spectroscopy at absorption peak of 536 nm occurred due to the surface Plasmon Resonance effect. The antimicrobial activity and mechanism of gold nanoparticles studies for the bacterial growth of gram-negative bacteria like E.coli, Pseudomonas aeruginosa and gram-positive bacteria like Bacillus subtilis respectively. Highly crystalline nanoparticles of size 10nm are confirmed from HR-TEM which supports with d spacing of XRD analysis. Further the characteristics of the obtained gold nanoparticles were studied using XRD, UV-Vis spectroscopy, Scanning Electron Microscopic, and High Resolution Transmission Electron microscopy and the results are presented in detail.