Nanolocalized single cell membrane nanoelectroporation

The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) Pub Date : 2014-04-13 DOI:10.1109/NEMS.2014.6908809

T. Santra, C. Lee, Srabani Kar, J. Borana, Pen-Cheng Wang, F. Tseng

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引用次数: 4

Abstract

Today single cell research is a great interest to analyze cell to cell or cell to environment behavior with their intracellular compounds, where bulk measurement can provide average value. To deliver biomolecules precise and localized way into single living cell with high transfection rate and high cell viability is a challenging and promisible task for biological and therapeutic research. In this report, we present a nano-localized single cell nano-electroporation technique, where electroporation take place in a very precise and localized area on a single cell membrane to achieve high efficient delivery with high cell viability. We fabricated 60nm gap with 40 nm triangular Indium Tin Oxide (ITO) based nano-eletcrode tip, which can intense electric field in a nano-localized area of a single cell to permeabilize cell membrane and deliver exogenous biomolecules from outside to inside of the cell. This device successfully deliver dyes, proteins into single cell with high cell viability (98%). The process not only control precise delivery mechanism into single cell with membrane reversibility, but also it can provide special, temporal and qualitative dosage control, which might be beneficial for therapeutic and biological cell studies.

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纳米定位单细胞膜纳米电穿孔

今天，单细胞研究是一个很大的兴趣，分析细胞对细胞或细胞对细胞内化合物的环境行为，其中批量测量可以提供平均值。如何以高转染率和高细胞活力的方式将生物分子精确、定位地传递到单个活细胞中，是生物学和治疗研究的一项具有挑战性和前景广阔的任务。在本报告中，我们提出了一种纳米定位单细胞纳米电穿孔技术，其中电穿孔发生在单个细胞膜上非常精确和定位的区域，以实现高细胞活力的高效递送。我们利用40 nm三角形氧化铟锡(ITO)纳米电极尖端制造了60nm的间隙，可以在单个细胞的纳米局部区域增强电场，使细胞膜通透，并将外源生物分子从细胞外部传递到细胞内部。该装置成功地将染料、蛋白质送入单细胞，细胞存活率高(98%)。这一过程不仅控制了精确的给药机制，具有膜可逆性，而且可以提供特殊的、时间的和定性的剂量控制，这可能有助于治疗和生物细胞的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

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