Pub Date : 2014-09-25DOI: 10.1109/NEMS.2014.6908879
Tao Yu, Mengge Li, Yu Xiang, Jinwen Zhang
This paper presented a novel sacrificial electrode dielectrophoresis method which was able to fabricate a large amount of totally discrete SWNT devices on a single chip synchronously. The discrete devices included a pair of W/Au opposed electrodes and SWNT bundles that were assembled between electrodes by DEP. We used Al lines to connect the two electrodes of all devices to two large Al pads. AC DEP bias were applied on Al pads and the SWNTs were self-assembled at the same time. Then Al was etched so that W/Au electrodes of the same polarity were disconnected and all devices were discrete electrically. The density of SWNT devices was 3 times larger than before. The electrical measurement showed that ohmic contact was formed between the assembled SWNTs and the electrodes. Our sacrificial electrode DEP method made it more flexible to achieve SWNT devices in large scale.
{"title":"Large scale and high yield assembly of SWNTs by sacrificial electrode method","authors":"Tao Yu, Mengge Li, Yu Xiang, Jinwen Zhang","doi":"10.1109/NEMS.2014.6908879","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908879","url":null,"abstract":"This paper presented a novel sacrificial electrode dielectrophoresis method which was able to fabricate a large amount of totally discrete SWNT devices on a single chip synchronously. The discrete devices included a pair of W/Au opposed electrodes and SWNT bundles that were assembled between electrodes by DEP. We used Al lines to connect the two electrodes of all devices to two large Al pads. AC DEP bias were applied on Al pads and the SWNTs were self-assembled at the same time. Then Al was etched so that W/Au electrodes of the same polarity were disconnected and all devices were discrete electrically. The density of SWNT devices was 3 times larger than before. The electrical measurement showed that ohmic contact was formed between the assembled SWNTs and the electrodes. Our sacrificial electrode DEP method made it more flexible to achieve SWNT devices in large scale.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"81 1","pages":"582-585"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74973716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-09-25DOI: 10.1109/NEMS.2014.6908892
A. Fraiwan, C. Dai, N. Sidhu, A. Rastogi, Seokheun Choi
Integration of electrochemical analytical functionality into a micro-sized microbial fuel cell is demonstrated. Screen-printed carbon ink based working (anode) and counter (cathode) electrodes and Ag/AgCl ink based reference electrode were deployed for sensing. Using such three-electrode configuration, in-situ cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were successfully performed to study the behavior of microbial electron transfer at the anode and to evaluate electrochemical properties of the MFC. In this work, coupling of electrochemical studies to the MFC platform has provided valuable information leading to a quantitative understanding of bacterial redox potential mass transport and the electrode/bacteria electron transfer kinetics.
{"title":"A micro-sized microbial fuel cell with electrochemical sensing functionality","authors":"A. Fraiwan, C. Dai, N. Sidhu, A. Rastogi, Seokheun Choi","doi":"10.1109/NEMS.2014.6908892","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908892","url":null,"abstract":"Integration of electrochemical analytical functionality into a micro-sized microbial fuel cell is demonstrated. Screen-printed carbon ink based working (anode) and counter (cathode) electrodes and Ag/AgCl ink based reference electrode were deployed for sensing. Using such three-electrode configuration, in-situ cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were successfully performed to study the behavior of microbial electron transfer at the anode and to evaluate electrochemical properties of the MFC. In this work, coupling of electrochemical studies to the MFC platform has provided valuable information leading to a quantitative understanding of bacterial redox potential mass transport and the electrode/bacteria electron transfer kinetics.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"18 1","pages":"635-638"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87702871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-09-25DOI: 10.1109/NEMS.2014.6908833
Jeng-Yi Lee, Ray-Kuang Lee
As the modern development of electronic device smaller and smaller, quantum effect would become significant. Thus extending the concept of quantum mechanics into design of new electronic devices becomes more important. Controlling the movement of these electrons plays the central role in this issue. In this work, we propose a new quantum cloaking mechanism which has completely different to the previous work of Liao et al [1]. This mechanism is based on the scattering cancellation and the interplay among the nodal points of partial waves, that one can simultaneously guide the probability flux outside the interior and keep the total scattering cross section negligible. Moreover, we can put any electric devices inside quantum cloaking without affecting outside probability of matter wave. With the analogy between quantum matter waves and classical waves, the concept of our method can be applied in other fields, such as electromagnetic and acoustic systems, etc.
{"title":"Quantum cloakings hide electronic devices","authors":"Jeng-Yi Lee, Ray-Kuang Lee","doi":"10.1109/NEMS.2014.6908833","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908833","url":null,"abstract":"As the modern development of electronic device smaller and smaller, quantum effect would become significant. Thus extending the concept of quantum mechanics into design of new electronic devices becomes more important. Controlling the movement of these electrons plays the central role in this issue. In this work, we propose a new quantum cloaking mechanism which has completely different to the previous work of Liao et al [1]. This mechanism is based on the scattering cancellation and the interplay among the nodal points of partial waves, that one can simultaneously guide the probability flux outside the interior and keep the total scattering cross section negligible. Moreover, we can put any electric devices inside quantum cloaking without affecting outside probability of matter wave. With the analogy between quantum matter waves and classical waves, the concept of our method can be applied in other fields, such as electromagnetic and acoustic systems, etc.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"38 1","pages":"386-389"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86181830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This paper reports three types of nanorobotic end-effectors: m@CNTs-based sphere-on-pillar (SOP) optical nanoantennas, nanotube fountain pen, and m@CNTs-based tunneling nanosensor. The fabrication method of the m@CNTs-based SOP optical nanoantennas we developed has potentials in the investigation of nano-optics and nano-photonics due to its feasibility in preparing such devices. Nanotube fountain pen (NFP) illustrates the practical applications in the direct fabrication of nanostructures from 0 to 3D, which is of critical importance in the future fast-prototype of nanodevices. The m@CNTs-based tunneling nanosensor provides a new design in measuring force/displacement in nanoscale, opening a new ground in developing nanoelectromechanical system (NEMS). These three end-effectors enable new functions for the nanorobotic manipulators and extending people's ability in exploring the world in nanoscale.
{"title":"Nanorobotic end-effectors: Design, fabrication, and in situ characterization","authors":"Zheng Fan, Miao Yu, Gautham Dharuman, Xudong Fan, Lixin Dong","doi":"10.1109/NEMS.2014.6908748","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908748","url":null,"abstract":"This paper reports three types of nanorobotic end-effectors: m@CNTs-based sphere-on-pillar (SOP) optical nanoantennas, nanotube fountain pen, and m@CNTs-based tunneling nanosensor. The fabrication method of the m@CNTs-based SOP optical nanoantennas we developed has potentials in the investigation of nano-optics and nano-photonics due to its feasibility in preparing such devices. Nanotube fountain pen (NFP) illustrates the practical applications in the direct fabrication of nanostructures from 0 to 3D, which is of critical importance in the future fast-prototype of nanodevices. The m@CNTs-based tunneling nanosensor provides a new design in measuring force/displacement in nanoscale, opening a new ground in developing nanoelectromechanical system (NEMS). These three end-effectors enable new functions for the nanorobotic manipulators and extending people's ability in exploring the world in nanoscale.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"462 1","pages":"6-11"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82993388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-09-25DOI: 10.1109/NEMS.2014.6908786
Yanqing Zhu, Lei Han, M. Qin, Qing‐An Huang, Ming-xia Jiang
This paper presents the design and testing results of an electrothermally driven MEMS (microelectromechanical systems) actuator. Different from conventional uni-directional thermal actuators, this in-plane bi-directional electrothermal actuator is capable of producing displacements in two directions as a single device. The RF MEMS switch driven by this cascaded electrothermal actuator is also proposed. Due to this bidirectional actuator, the proposed switch can not only realize the OFF-state to ON-state shifting but also provide an additional deep OFF-state. At the deep OFF state, the isolation better than -30 dB can be achieved at the whole frequency range of 0 ~ 40 GHz.
{"title":"Development of a novel bidirectional electrothermal actuator and its application to RF MEMS switch","authors":"Yanqing Zhu, Lei Han, M. Qin, Qing‐An Huang, Ming-xia Jiang","doi":"10.1109/NEMS.2014.6908786","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908786","url":null,"abstract":"This paper presents the design and testing results of an electrothermally driven MEMS (microelectromechanical systems) actuator. Different from conventional uni-directional thermal actuators, this in-plane bi-directional electrothermal actuator is capable of producing displacements in two directions as a single device. The RF MEMS switch driven by this cascaded electrothermal actuator is also proposed. Due to this bidirectional actuator, the proposed switch can not only realize the OFF-state to ON-state shifting but also provide an additional deep OFF-state. At the deep OFF state, the isolation better than -30 dB can be achieved at the whole frequency range of 0 ~ 40 GHz.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"42 1","pages":"180-185"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78996157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-09-25DOI: 10.1109/NEMS.2014.6908785
Chun-Ting Lin, M. Chang, Yu-Wei Chen, Shu-Hung Tung, Nancy Chu, C. Hsiao, M. Shiao
In this paper, a platinum-silver composite nano-dot (Pt@Ag ND) decorated tip apex was successfully fabricated by utilizing a localized two-step galvanic replacement. In the first replacement, Ag-ND tip was prepared by the replacement between silicon and silver ion through fluoride assisted galvanic replacement reaction. In the second replacement, the Ag ND was further changed to Pt by the interaction between Ag and chloroplatinic acid. Our preliminary results demonstrated the possibility of preparing either a single metal-nanodot (metal-ND) or core-shell structure modified tip apexes for field sensitive scanning probe microscopy (FS-SPM) applications.
{"title":"Localized two-step galvanic replacement of a tip apex modification for field sensitive scanning probe microscopy","authors":"Chun-Ting Lin, M. Chang, Yu-Wei Chen, Shu-Hung Tung, Nancy Chu, C. Hsiao, M. Shiao","doi":"10.1109/NEMS.2014.6908785","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908785","url":null,"abstract":"In this paper, a platinum-silver composite nano-dot (Pt@Ag ND) decorated tip apex was successfully fabricated by utilizing a localized two-step galvanic replacement. In the first replacement, Ag-ND tip was prepared by the replacement between silicon and silver ion through fluoride assisted galvanic replacement reaction. In the second replacement, the Ag ND was further changed to Pt by the interaction between Ag and chloroplatinic acid. Our preliminary results demonstrated the possibility of preparing either a single metal-nanodot (metal-ND) or core-shell structure modified tip apexes for field sensitive scanning probe microscopy (FS-SPM) applications.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"344 1","pages":"176-179"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76408601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-09-25DOI: 10.1109/NEMS.2014.6908795
Zhuo Li, Xinwu Xie, Weixing Chen, Weiran Liu, Ran Li
A microfluidic device for single mouse embryo manipulation was fabricated and the impedance spectroscopy of the positioned embryo was detected by the electrodes in the microchannel. Experiment results showed that the impedance of embryo varies when it is at different stage. Also, healthy embryos and development-blocked embryos showed different impedance spectroscopy. Compared with the traditional means of development evaluation in clinic, sensing of impedance is a totally new method for embryo development with the benefit of label free, quantifiable, real-time monitoring and had great potential to be used in development status prediction in clinic.
{"title":"On-chip manipulating and impedance spectroscopy sensing of single mouse embryo","authors":"Zhuo Li, Xinwu Xie, Weixing Chen, Weiran Liu, Ran Li","doi":"10.1109/NEMS.2014.6908795","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908795","url":null,"abstract":"A microfluidic device for single mouse embryo manipulation was fabricated and the impedance spectroscopy of the positioned embryo was detected by the electrodes in the microchannel. Experiment results showed that the impedance of embryo varies when it is at different stage. Also, healthy embryos and development-blocked embryos showed different impedance spectroscopy. Compared with the traditional means of development evaluation in clinic, sensing of impedance is a totally new method for embryo development with the benefit of label free, quantifiable, real-time monitoring and had great potential to be used in development status prediction in clinic.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"15 1","pages":"220-224"},"PeriodicalIF":0.0,"publicationDate":"2014-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88856609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908826
L. Wen, Han Xie, J. Chu, Hai Wang
This paper reports a novel maskless nanoscale material etching method based on microplasma devices arrays. That is, inverted pyramidal microplasma devices arrays are integrated into the scanning probe tips array to realize maskless nanoscale material etching with advantages of high efficiency, large area and low cost. A 4×4 inverted pyramidal microplasma device array with each microcavity dimension of 50μm was successfully fabricated by MEMS process. Experiment results showed that the microplasma devices arrays could ignite in rare gas Ar under dc excitation. Ballast resistance in electrical testing system played an important roles in device array ignitions. V-I characteristics of the device array at 10kpa of Ar was in negative glow discharge mode. This work may lay a good foundation for future maskless microplasma nanoscale material etching.
{"title":"Development of microplasma devices arrays for maskless nanoscale material etching","authors":"L. Wen, Han Xie, J. Chu, Hai Wang","doi":"10.1109/NEMS.2014.6908826","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908826","url":null,"abstract":"This paper reports a novel maskless nanoscale material etching method based on microplasma devices arrays. That is, inverted pyramidal microplasma devices arrays are integrated into the scanning probe tips array to realize maskless nanoscale material etching with advantages of high efficiency, large area and low cost. A 4×4 inverted pyramidal microplasma device array with each microcavity dimension of 50μm was successfully fabricated by MEMS process. Experiment results showed that the microplasma devices arrays could ignite in rare gas Ar under dc excitation. Ballast resistance in electrical testing system played an important roles in device array ignitions. V-I characteristics of the device array at 10kpa of Ar was in negative glow discharge mode. This work may lay a good foundation for future maskless microplasma nanoscale material etching.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"10 1","pages":"358-361"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74767719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-04-13DOI: 10.1109/NEMS.2014.6908819
Po-Yu Peng, W. Hsu, T. Pan, Yen-Heng Lin
We propose a method that can be used to quantitatively measure concentrations of protein through a semiconductor sensor with a robust signal. Measurement of protein by a semiconductor may encounter a major problem. For specifically measuring protein, an antibody is immobilized on the semiconductor sensor surface. The physical length of the antibody is around 10 nm, a distance too long to be sensed by the semiconductor sensor, which means that the following attached protein cannot be sensed. In this study, we used a bead-based immunoassay combined with the DNA strain labeling technique to overcome this issue. Protein was first captured using an antibody-coated magnetic bead. It was then labeled with a secondary antibody combined with the DNA strain. Finally, the magnetic bead with the biotarget was attracted on the sensor surface by an external magnetic field, and then the negative charges of the DNA changed the surface potential of the sensor. The concentration of protein could then be measured accordingly. The signal could be further improved by the optimization of the number of labeling DNA and the size of the magnetic bead. In addition, the semiconductor sensor was incorporated in a microfluidic chip with microvalves and a micromixer. The microfluidic procedure reduced the total measurement time to around 1 h (plate ELISA 4h). Furthermore, by changing the antibody to another type of antibody coated on the magnetic bead, the sensor could be reused to measure other types of protein. The proposed method provides a solution for the robust measurement of protein concentration through the semiconductor sensor.
{"title":"Rapid detection of bladder cancer using an immunoassay transistor combined with DNA-labeling technique in a microfluidic chip","authors":"Po-Yu Peng, W. Hsu, T. Pan, Yen-Heng Lin","doi":"10.1109/NEMS.2014.6908819","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908819","url":null,"abstract":"We propose a method that can be used to quantitatively measure concentrations of protein through a semiconductor sensor with a robust signal. Measurement of protein by a semiconductor may encounter a major problem. For specifically measuring protein, an antibody is immobilized on the semiconductor sensor surface. The physical length of the antibody is around 10 nm, a distance too long to be sensed by the semiconductor sensor, which means that the following attached protein cannot be sensed. In this study, we used a bead-based immunoassay combined with the DNA strain labeling technique to overcome this issue. Protein was first captured using an antibody-coated magnetic bead. It was then labeled with a secondary antibody combined with the DNA strain. Finally, the magnetic bead with the biotarget was attracted on the sensor surface by an external magnetic field, and then the negative charges of the DNA changed the surface potential of the sensor. The concentration of protein could then be measured accordingly. The signal could be further improved by the optimization of the number of labeling DNA and the size of the magnetic bead. In addition, the semiconductor sensor was incorporated in a microfluidic chip with microvalves and a micromixer. The microfluidic procedure reduced the total measurement time to around 1 h (plate ELISA 4h). Furthermore, by changing the antibody to another type of antibody coated on the magnetic bead, the sensor could be reused to measure other types of protein. The proposed method provides a solution for the robust measurement of protein concentration through the semiconductor sensor.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"21 1","pages":"329-332"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81606072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oocyte enucleation (OE) is a requisite step in animal clone. The traditional OE manipulation requires high professional skills and involves contaminations easily. To address these problems, a robotic enucleation process for oocytes is proposed using traditional micro-operation system. Using imaging processing and motion control, key procedures of OE process, such as global location of oocytes, target cell immobilization, cell-orientation adjustment, cell penetration, and nuclei removing, are realized automatically or robotically. Experimental results demonstrate: 1) this system is capable of performing oocyte enucleation at average speed of 82s/cell; 2) the success rate of totally-removing genetic materials using our method is up to 90%; 3) the development competence of the embryos operated by this method is comparable to those operated by manual operation.
{"title":"Robotic enuleation for oocytes","authors":"Qili Zhao, Maosheng Cui, Chunyang Zhang, Jin Yu, Mingzhu Sun, Xin Zhao","doi":"10.1109/NEMS.2014.6908751","DOIUrl":"https://doi.org/10.1109/NEMS.2014.6908751","url":null,"abstract":"Oocyte enucleation (OE) is a requisite step in animal clone. The traditional OE manipulation requires high professional skills and involves contaminations easily. To address these problems, a robotic enucleation process for oocytes is proposed using traditional micro-operation system. Using imaging processing and motion control, key procedures of OE process, such as global location of oocytes, target cell immobilization, cell-orientation adjustment, cell penetration, and nuclei removing, are realized automatically or robotically. Experimental results demonstrate: 1) this system is capable of performing oocyte enucleation at average speed of 82s/cell; 2) the success rate of totally-removing genetic materials using our method is up to 90%; 3) the development competence of the embryos operated by this method is comparable to those operated by manual operation.","PeriodicalId":22566,"journal":{"name":"The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"112 1","pages":"23-27"},"PeriodicalIF":0.0,"publicationDate":"2014-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81014171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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