Agricultural Bio-Stimulant Activity of Fish Protein Hydrolysate from Common Silver Belly (Gerres subfasciatus)

S. S., D. R., S. M
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Abstract

Taking into account of using the fish industry wastes and for expanding the incentive to a few under used fish species, protein hydrolysates from fish proteins are being set up by a few specialists everywhere throughout the world. Fish Protein Hydrolysates (FPH) are breakdown results of enzymatic transformation of fish proteins into smaller units like peptides, which typically contain 2-20 amino acids. Their worth has been expanded by utilizing them as fluid bio compost, feed supplement and bioorganic fertilizer. From the research carried out at our laboratory, Dry fish silver belly with both microbe and enzyme acquired from cultural broth of lactobacillus species, catalyst papain demonstrated positive upgrade of development and advancement of plants tested. In view of the yield of FPH concentrates from both microbial and enzymatic hydrolysis, the pace of seedling life list in Urad dhal, green gram and okra got higher in 0.1-0.5% convergence of dry fish hydrolysate from lactobacillus treated than in the control variation. With respect to the seeds of urad dhal, green gram and okra, the germination rate and seedling energy list from treated seeds surpassed control by 19.11-128.71%. The positive effect of dry fish protein hydrolysate by microbial procedure on germination rate, seedling life record rate in 3 kinds of seeds is the reason for the improvement of protein hydrolysate item for application in crop creation utilizing dry fish squanders. The Seedling grown in 0.5% concentration of fish hydrolysates showed significant increase in seedling vigour index of Urad dhal (13.29 ± 0.12 %), Green gram (14.1 ± 0.17%) and Okra (5.39 ± 0.43 %) was recorded through one way Analysis of Variance (ANOVA). By HPLC determination amino acid results determined that protein hydrolysates process can be stopped on 7th day (643.56ppm) whereas @ 10th day amino acid decreased (326.24ppm). Results suggest that probiotic assumes a superior job in dry fish protein hydrolysates than compounds when contrasted and yield and prudent astute.
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银腹鱼蛋白水解物的农业生物刺激素活性
考虑到利用渔业废料和扩大对少数未充分利用的鱼类的奖励,世界各地的一些专家正在从鱼类蛋白质中提取蛋白质水解物。鱼蛋白水解物(FPH)是酶将鱼蛋白转化为更小的单位(如肽)的分解结果,通常含有2-20个氨基酸。利用它们作为液体生物堆肥、饲料添加剂和生物有机肥,扩大了它们的价值。从我们实验室开展的研究中,从乳酸菌种的培养肉汤中获得微生物和酶,催化剂木瓜蛋白酶,干鱼银肚显示了被测植物的积极发展和进步。从微生物酶解和酶解的FPH浓缩液产量来看,乳杆菌发酵的鱼干水解液在0.1-0.5%的收敛量下,乌拉尔、绿克和秋葵的幼苗寿命表速度高于对照变化。乌拉尔、绿克和秋葵种子的发芽率和幼苗能表比对照高19.11% ~ 128.71%。经微生物处理的鱼干蛋白水解物对3种种子的发芽率和幼苗寿命记录率的积极影响,是改进鱼干废弃物作物生产中应用的蛋白水解物项目的原因。经单因素方差分析(ANOVA),在0.5%浓度的鱼解液中培养的幼苗活力指数显著提高了乌拉尔(13.29±0.12%)、绿克(14.1±0.17%)和秋葵(5.39±0.43%)。高效液相色谱法测定氨基酸结果表明,蛋白水解过程在第7天可以停止(643.56ppm),而在第10天氨基酸含量下降(326.24ppm)。结果表明,在鱼干蛋白水解物中,益生菌的作用优于化合物。
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