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Post-Harvest Fungi of Vitellaria paradoxa and Parkia biglosa in Chad Republic and Bioactivity of Natural Products against Some Pathogenic Fungi 乍得产双头牛蒡和大枇杷采后真菌及其天然产物对几种病原菌的生物活性研究
Pub Date : 2023-03-08 DOI: 10.26420/annagriccropsci.2023.1126
Djeugap FJ, Labassou HD, Essomo ES, Sonkoue MA, Serferbe S
In Chad Republic, kernels/grains of Vitellaria paradoxa and Parkia biglobosa are two Edible Non-Timber Forest Products (ENTFP) with high economic value. These products are colonized by un-identified post-harvest fungi that are responsible for high post-harvest losses. The objective of the study was to contribute to the management of the post-harvest diseases of kernels of V. paradoxa and P. Biglosa through natural products. To achieve this, post-harvest fungi were isolated from infected kernels and their pathogenicity tested. Then, antifungal activity of Essential Oil (EO) of Thymus algeriensis and crude extract of African panaxia was carry out by the dispersion method on the agar medium on four pathogenic fungi isolated from the two infected ENTFP. Results showed that V. paradoxa kernels were highly infected (77-95%) compared to P. biglobosa (0.6-2.6%). Fungal species frequently associated with V. paradoxa and P. Biglobosa kernels were: Aspergillus niger (46%), Rhizopus nigricans (17%), Oidium sp (22%) and Cercospora sp (8%); and Oidium sp (55%), A. niger (18%), A. flavus (18%) and Cercospora sp (6%) in V. paradoxa and P. biglobosa respectively. Pathogenicity test was positive with all the species belonging to the genus Aspergillus and with Oidium sp. Essential oil of T. vulgaris at 1.5 �l/ml and the crude extract of African panaxia at 120 �g/ml totally inhibited the growth of the four potential mycotoxigenic fungi tested; their efficacy were significantly comparable (p<0.05) to the reference fungicide (Terazeb). In vivo control of post-harvest diseases with these two natural products is being carry out.
在乍得共和国,牛粪(Vitellaria paradoxa)和大白沙(Parkia biglobosa)的籽粒是两种具有较高经济价值的可食用非木材林产品。这些产品被未鉴定的收获后真菌定植,这些真菌负责收获后的高损失。本研究的目的是通过天然产物对双歧弧菌和双歧弧菌的收获后病害进行管理。为了实现这一目标,从感染的玉米粒中分离出收获后的真菌,并对其致病性进行了测试。然后,采用琼脂培养基分散法对两株感染ENTFP的病原菌分离的四种病原菌进行了阿尔及利亚胸腺精油和非洲人参粗提物的抑菌活性研究。结果表明,悖论弧菌侵染率(77 ~ 95%)高于大叶弧菌侵染率(0.6 ~ 2.6%)。与异长弧菌和大叶弧菌籽粒相关的真菌种类有:黑曲霉(46%)、黑根霉(17%)、黄霉(22%)和麻孢(8%);而在异叶弧菌和大叶弧菌中,分别有黑弧菌(55%)、黑弧菌(18%)、黄弧菌(18%)和黑弧菌(6%)。对曲霉属真菌的致病性试验均呈阳性,对黄霉属真菌的致病性试验均呈阳性。1.5 μ l/ml的黄霉精油和120 μ g/ml的非洲人参粗提物对4种潜在产毒真菌的生长均有抑制作用;其药效与参比杀菌剂Terazeb显著相当(p < 0.05)。目前正在用这两种天然产物进行收获后疾病的体内控制。
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引用次数: 0
Challenges and Opportunities Faced on Food Production Systems in Arid and Semi-Arid Regions under Climate Change Conditions 气候变化条件下干旱和半干旱地区粮食生产系统面临的挑战和机遇
Pub Date : 2022-02-09 DOI: 10.26420/annagriccropsci.2022.1109
Jahan M, Ghalenoei Sh
Climate change and variability may have an impact on the occurrence of food safety hazards at various stages of the food chain. It may also affect socio-economic aspects related to food systems such as agriculture, animal production, global trade, demographics and human behavior which all influence food safety. There is also concern that meeting the rising demand for food is leading to environmental degradation thereby exacerbating factors in part responsible for climate change, and further undermining the food systems upon which food security is based. A major emphasis of climate change/food security research over recent years has addressed the agronomic aspects of climate change, and particularly crop yield. Therefore, while agronomic research alone cannot address all food security/climate change issues, hence the balance of investment in research and development for crop production vis a vis other aspects of food security needs to be assessed. Improved understanding of the impacts of climate change on crop production helps to develop adaptation options, and also crucially it improves understanding of the consequences of different adaptation options on further climate forcing. This role can further be strengthened if agronomists work alongside other scientists to develop adaptation options that are not only effective in terms of crop production, but are also environmentally and economically robust, at landscape and regional scales. Furthermore, such integrated approaches are much more likely to address the information need of policy makers. The potential for stronger linkages between the results of agronomic research and the policy environment will thus be enhanced.
气候变化和变率可能对食品链各个阶段的食品安全危害的发生产生影响。它还可能影响与粮食系统有关的社会经济方面,如农业、动物生产、全球贸易、人口统计和人类行为,这些都影响食品安全。人们还担心,满足不断增长的粮食需求正在导致环境退化,从而加剧了部分导致气候变化的因素,并进一步破坏了粮食安全所依赖的粮食系统。近年来,气候变化/粮食安全研究的主要重点是气候变化的农艺方面,特别是作物产量。因此,虽然仅靠农艺研究无法解决所有粮食安全/气候变化问题,但需要评估作物生产研发投资与粮食安全其他方面之间的平衡。更好地了解气候变化对作物生产的影响有助于制定适应方案,而且至关重要的是,它还有助于更好地了解不同适应方案对进一步气候强迫的影响。如果农学家与其他科学家合作,制定适应方案,不仅在作物生产方面有效,而且在景观和区域尺度上在环境和经济上也很强大,那么这一作用可以进一步加强。此外,这种综合办法更有可能满足决策者的信息需要。因此,将加强农艺研究结果与政策环境之间更紧密联系的可能性。
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引用次数: 1
Integrated Management of Peanut Foliar Diseases by Cercospora, Alternaria and Web Blotch 花生斑孢、互交和网斑病的综合治理
Pub Date : 2022-01-31 DOI: 10.26420/annagriccropsci.2022.1108
Parvin Ms, Haque Mde, A. F, Shafin Ms
The concept of integrated management was first conceptualized in the 1950s by entomologists to describe the selection of specific pesticides with adjusted dosages and application timings to control harmful insects and mites while preserving beneficial insects. However, the management of disease conveys the idea of a continuous process that is more preventative in which the loss from disease is kept below some economic level. Over the decades, the concept was eventually expanded to include all relevant biological, cultural, and chemical tactics to manage insect, pathogen and weed pests of crops; a multi-tactic approach to pest management. Notably, the concept of plant health management was taken up by the American Phytopathological Society in the early ’90s, which resulted in series of Plant Health Management publications such as “Wheat health Management” and “Potato Health Management”. In this review article, we will discuss the closely associated pathogens included Cercospora, Alternaria, and Web blotch, which cause peanut foliar disease, and its management strategies.
综合管理的概念最早是在20世纪50年代由昆虫学家提出的,描述了选择特定的农药,调整剂量和施用时间,以控制有害昆虫和螨虫,同时保护有益昆虫。然而,疾病管理传达的理念是一个持续的过程,更具有预防性,其中疾病的损失保持在某种经济水平以下。几十年来,这一概念最终扩展到包括所有相关的生物、文化和化学策略,以管理作物的昆虫、病原体和杂草害虫;害虫管理的多策略方法。值得注意的是,植物健康管理的概念在90年代初被美国植物病理学学会采纳,并产生了一系列植物健康管理出版物,如“小麦健康管理”和“马铃薯健康管理”。在这篇综述文章中,我们将讨论密切相关的病原体,包括Cercospora, Alternaria和Web blotch,引起花生叶病及其管理策略。
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引用次数: 0
Challenges and Opportunities for the Global Cultivation and Adaptation of Legumes B. Opportunities for Increasing Legumes Production and Availability 豆科植物全球种植与适应的挑战与机遇B.增加豆科植物产量和可得性的机遇
Pub Date : 2022-01-28 DOI: 10.26420/annagriccropsci.2022.1107
A. Wf, Hegab Rh, Ellington Ef
Legumes cultivation is subjected to different constraints, which reduce productivity, particularly effects of global warming, and other constraints. While the large diversity of legumes play an important contributing role to food and nutrition security by the sustainable agriculture (crop and livestock systems) and food systems worldwide. Improving legumes production needs numerous strategies achievement proper production for humanity. Through different ways that include policy creativities to encourage legume cultivation, produce legume varieties adapted to changing climatic conditions. In addition to using proper agricultural strategies to increase the availability of legumes like increasing annual cultivation by both horizontal extensions by increasing planted area and reclamation the poor soil and using intensive planting system as a Vertical development tool. Besides, planting legumes with other crops in the intercropping system, as well as involving legumes into the annual agriculture system, improving postharvest processing to minimize crop losses. Furthermore, using modern technology in agriculture like smart agriculture to increase legumes productivity. There are many health benefits to legume crops due to their component, particularly protein, which reaches two or three-fold as in other crops such as cereals, which contain less than half of the protein in legumes. In addition to carbohydrates, folic acid, fibers, also, legumes are considered low-fat seeds. In addition, to using leguminous crops as fodder resources for animals both directly and as a part of different feeds for livestock and poultry.
豆科作物的种植受到不同的限制,特别是全球变暖的影响和其他限制,从而降低了生产力。豆类的丰富多样性通过可持续农业(作物和牲畜系统)和全球粮食系统对粮食和营养安全发挥着重要的促进作用。提高豆科作物产量需要多种策略,以实现适合人类的生产。通过不同的方式,包括鼓励豆类种植的政策创新,生产适应不断变化的气候条件的豆类品种。除了使用适当的农业策略来增加豆科植物的可用性,如通过增加种植面积和开垦贫瘠土壤的水平扩展来增加年种植,并使用集约种植系统作为垂直发展工具。此外,在间作系统中与其他作物一起种植豆科作物,并将豆科作物纳入年度农业系统,改进采后加工,尽量减少作物损失。此外,在农业中使用智能农业等现代技术来提高豆科作物的产量。豆类作物由于其成分,特别是蛋白质,对健康有许多好处,其蛋白质含量是谷类等其他作物的两到三倍,而谷类的蛋白质含量不到豆类的一半。除了碳水化合物、叶酸、纤维,豆类也被认为是低脂肪的种子。此外,利用豆科作物直接作为动物饲料资源或作为不同畜禽饲料的一部分。
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引用次数: 0
Traditional Livestock System and Improvement of Cow’s Hygienic Milk Quality Sold in N’Djamena, Chad 在乍得恩贾梅纳销售的传统畜牧系统和奶牛卫生牛奶质量的改进
Pub Date : 2022-01-27 DOI: 10.26420/annagriccropsci.2022.1106
B. M, Makhlouf H, K. D., Tidjani A
Unlike most Sahelian towns, the production and selling of milk in N’Djamena is generally made in a traditional way and causes a serious hygiene and public health problem. This survey aims to improve the hygienic quality of milk by reducing contamination at critical points in the milk production chain. The approach is based on using suitable, cleaning and disinfection equipment’s of milking, collection and storage. The results showed contamination had significantly decreased by comparing the situation before and after interventions. The average of total mesophilic flora felt from 3106 to 104, total coliforms from 6.8103 to 3.9101; thermotolerant coliforms from 2101 to 0.6101 and Staphylococcus from 1.07102 to 1.5101. The pH increased from 6.55 to 6.6 and the dornic acidity from 20 to 21. The investment cost is 15 FCFA/day in the village with 146 liters of milk sold at 275 FCFA per unit. It is 12 FCFA/day per dairy unit for 324.6 liters sold at 533.33 FCFA per unit. The model for improving the hygienic quality of milk sold in N’Djamena improved the physico-chemical qualities of milk and significantly reduced the rate of microbial contamination. The general hygiene improvement and especially personal hygiene quality was weak because it is very difficult to change traditional behavior, but the boil milk action contributed to reduce the initial contamination.
与大多数萨赫勒城镇不同,恩贾梅纳的牛奶生产和销售一般采用传统方式,造成了严重的卫生和公共健康问题。这项调查旨在通过减少牛奶生产链关键环节的污染来提高牛奶的卫生质量。该方法是基于使用合适的挤奶、收集和储存的清洁和消毒设备。结果表明,干预前后污染明显减少。中温菌群总数平均值为3106 ~ 104,大肠菌群总数平均值为6.8103 ~ 3.9101;耐热大肠菌群为2101 ~ 0.6101,葡萄球菌为1.07102 ~ 1.5101。pH由6.55增加到6.6,酸性由20增加到21。在村里,投资成本为每天15澳新台币,146升牛奶以每瓶275澳新台币的价格出售。324.6升的奶制品售价为533.33 FCFA/单位,每单位12 FCFA/天。改善恩贾梅纳销售的牛奶卫生质量的模型改善了牛奶的物理化学质量,并显著降低了微生物污染率。由于传统行为难以改变,总体卫生改善特别是个人卫生质量较弱,但煮奶行为有助于减少初始污染。
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引用次数: 0
Is it Sustainable to Cultivate a Monoculture of Durum Wheat with Prolonged No-Tillage Management? 长期免耕单一栽培硬粒小麦是否可持续?
Pub Date : 2022-01-10 DOI: 10.26420/annagriccropsci.2022.1105
T. A., R. M, F. R.
The prolonged effect of no-tillage (NT) and conventional tillage (CT) treatment on durum wheat (Triticum durum Desf.), continuously grown in Southern Italy, has been evaluated in experimental fields for sixteen years (1994/95-2009/10) to ascertain whether the yields and quality of the grain as well as some soil characteristics had changed. The average grain yield in CT (2.70 ± 0.96 t ha-1) and NT (2.63 ± 0.74 t ha-1) treatment was not significant. CT treatment showed higher values of plant height and grain weight (74.2 cm and 43.2 g) than NT (71.0 cm and 41.8 g). Regarding to the semolina quality, the values of the gluten index and the dough strength (W parameter) as well as the protein content and the yellow index were not significantly different for the NT and CT treatments. As for soil moisture, the NT treatment stored in the soil profile, and over time, about 13% more water than the CT one. In the upper soil layer of the NT treatment the SOC value (16.0 ± 2.2 g kg-1) was 13.2% higher than CT one (14.2 ± 1.2 g kg-1) while the CT treatment (13.7 ± 0.5 g kg-1) showed in the lower soil layer a SOC value of 30.2% higher than the NT one (9.6 ± 1.9 g kg-1). Hence the prolonged no-tillage adoption in conditions of continuous durum wheat cultivation, even with slight losses in yield but not in the quality of grain or semolina, would seem sustainable.
对意大利南部连续种植的硬粒小麦(Triticum durum Desf.)进行了为期16年(1994/95-2009/10)的免耕(NT)和常规耕作(CT)处理的长期效果评估,以确定籽粒产量和质量以及一些土壤特征是否发生了变化。CT(2.70±0.96 t ha-1)和NT(2.63±0.74 t ha-1)处理的平均籽粒产量不显著。粗粒小麦的株高(74.2 cm)和粒重(43.2 g)均高于粗粒小麦(71.0 cm)和粗粒小麦(41.8 g)。粗粒小麦品质方面,粗粒小麦面筋指数、面团强度(W参数)、蛋白质含量和黄指数在粗粒小麦品质上无显著差异。在土壤水分方面,NT处理在土壤剖面中储存的水分,随着时间的推移,比CT处理多13%左右。土壤表层土壤有机碳(16.0±2.2 g kg-1)比土壤表层有机碳(14.2±1.2 g kg-1)高13.2%,土壤表层有机碳(13.7±0.5 g kg-1)比土壤表层有机碳(9.6±1.9 g kg-1)高30.2%。因此,在连续种植硬粒小麦的条件下,即使产量略有下降,但谷物或粗粒粉的质量却没有下降,长期采用免耕似乎是可持续的。
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引用次数: 2
The Role of Balanced Fertilization on the Reduction of Phytic Acid to Zinc Molar Ratio (PA/Zn) in the Irrigated and Rainfed Wheat Fields on the Calcareous Soils of Iran 平衡施肥对伊朗钙质土壤灌溉和旱作麦田植酸锌摩尔比降低的作用
Pub Date : 2022-01-05 DOI: 10.26420/annagriccropsci.2022.1104
Malakouti Mj, Bybordi A, Bahrami A, A. Skh
One of the most important quality factors in the wheat grain, which directly affects the quality of the bread, is the molar ratio of phytic acid to zinc (PA/ Zn) index. In order to evaluate the role of balanced fertilization on increasing the yield, quality, and reducing PA/Zn in wheat grain (Triticum aestivum L.), the first experiment was implemented with two fertilizer treatments (Farmer’s conventional fertilization practice, i.e. NP-fertilizers) and the optimum use of fertilizers (fertilization was on the basis of soil test results) in three different irrigated farms in Mianeh, East Azarbaijan province and the second experiment was carried out in 10 different dryland farms on the outskirts of the Khodabandeh, Zanjan Province in 2010-2011 growing season with an average of 380 mm annual rainfall. This experiment had three treatments, i.e. T1=Control (no fertilizer); T2=Farmer’s conventional fertilization practice (NP-fertilizers), and T3=Fertilization based on soil test values (Optimum use of fertilizers) in four replications with a randomized complete block design. The wheat cultivar in the irrigated farms was Alvand and in the rainfed farms was Azar-2. Protein, phytic acid, phosphorus (P), potassium (K), magnesium (Mg), iron (Fe), manganese (Mn), copper (Cu), and Zinc (Zn) in wheat grains were analyzed according to the standard methods. The collected data were analyzed by using a t-test and SAS Software. The results revealed that in the irrigated wheat fields, the optimal use of fertilizers increased the grain yield up to 27 percent, protein content, all micronutrients especially Zn significantly, but it decreased PA/Zn molar ratio significantly in comparison to Farmer’s conventional fertilization practice. While phytic acid concentration in the control plots was 8.70, it decreased up to 7.41gkg-1, Zn concentration was increased from 29 to 43 mgkg-1 and subsequently, PA/Zn molar ratio was reduced from 30 to 17. The statistical analysis showed a significant difference for phytic acid, Zn, and PA/Zn molar ratio in all wheat farms in the second experiment, the results demonstrated that while the average grain yield in T1 was 975kgha-1, it has increased up to 1401kgha-1 in T2 and over 1628kgha-1, respectively. The average yield increase was significant at the 5% level despite the existing drought situation in the fall of 2010. The mean concentration of P and Zn in wheat grains in the control plots were 0.26 percent and 21mgkg-1, in Farmer’s conventional fertilization practice were 0.24 percent and 25mgkg-1 and in the fertilization based on soil test values were 0.21 percent and 30mgkg-1, respectively and they were significant in the 5% level. While PA/Zn in the control plots was 29, it has been decreased to 22 in T2 and to 15 in T3 plots, respectively. Comparison between PA/Zn molar ratios revealed that due to the existing water during the growing season in the irrigated fields, the availability of all nutrients including Zn was slightly higher, b
小麦籽粒中最重要的品质因素之一是植酸锌摩尔比(PA/ Zn)指数,它直接影响面包的品质。为了评价平衡施肥对小麦籽粒(Triticum aestivum L.)增产、品质和降低PA/Zn的作用,在棉内省3个不同的灌溉农田进行了两种施肥处理(农民常规施肥方式,即np肥)和肥料优化利用(根据土壤试验结果施肥)的试验。2010-2011年生长季,在Zanjan省Khodabandeh郊区的10个不同旱地农场进行了第二次试验,年平均降雨量为380毫米。本试验设3个处理,T1=对照(不施肥);T2=农户的常规施肥做法(np肥料),T3=基于土壤试验值的施肥(肥料的最佳利用),采用随机完全区组设计,共4个重复。灌溉农田小麦品种为Alvand,旱作农田小麦品种为Azar-2。按标准方法对小麦籽粒中的蛋白质、植酸、磷(P)、钾(K)、镁(Mg)、铁(Fe)、锰(Mn)、铜(Cu)、锌(Zn)进行了分析。收集的数据采用t检验和SAS软件进行分析。结果表明:与传统施肥方式相比,优化施肥方式可使小麦籽粒产量提高27%,蛋白质含量、各微量元素尤其是锌含量显著提高,但PA/Zn摩尔比显著降低。对照区植酸浓度为8.70时,植酸浓度下降到7.41 mg -1,锌浓度从29 mg -1增加到43 mg -1, PA/Zn摩尔比从30降低到17。统计分析表明,在第二次试验中,各小麦农场植酸、锌和PA/Zn的摩尔比差异显著,结果表明,T1的平均产量为975kga -1,而T2的平均产量分别增加到1401kga -1和1628kga -1以上。尽管2010年秋季存在干旱情况,但平均产量仍显著提高了5%。对照区小麦籽粒中磷、锌的平均浓度分别为0.26%和21mg -1,农户常规施肥分别为0.24%和25mg -1,土壤试验施肥分别为0.21%和30mg -1,在5%水平下均达到显著水平。对照区PA/Zn为29,T2和T3区分别降至22和15。PA/Zn摩尔比的比较表明,由于灌区生长季节存在水分,包括Zn在内的所有养分的有效性略高,但在旱地,降雨量和分布非常重要,显然,增加生长季节的降雨量和分布,可以提高粮食产量,降低PA/Zn摩尔比。很明显,这些变化在很大程度上取决于气候变化、品种和管理因素。建议至少在伊朗的小麦种植省份继续实施这一项目。
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引用次数: 1
Effect of Different Sources of Phosphate Fertilizers on Cadmium Accumulation in Potato Tubers (Solanum tuberosum L.) in the Calcareous Soils of Iran 不同磷肥来源对伊朗钙质土壤马铃薯块茎中镉积累的影响
Pub Date : 2022-01-01 DOI: 10.26420/annagriccropsci.2021.1101
Karimi H, Malakouti Mj, D. M, A. Skh
Potato (Solanum tuberosum L.) is the fourth most important agricultural crop after wheat, rice, and maize around the world, so increasing its quality (with a low amount of contaminants) is very important and notable. If for any reason, Cadmium (Cd) concentration increases in the soil, its uptake by the potato will be increased as a result. One of the main sources of Cd in the soil is the application of imbalanced fertilization especially consuming imported phosphate fertilizers. In order to investigate the effect of different sources of P-fertilizers on the Cd accumulation in potato tubers, an experiment was carried out on a farm in Dehgolan, Iran. This experiment was conducted based on a Randomized Complete Block Design (RCBD) with three replications. Before starting the experiment, soil and P-fertilizer samples were taken and analyzed in the laboratory. The concentration of soil available P, Zn, and Cd were 8.0, 0.75, and 0.15 mg kg-1 respectively and the concentration of Cd in different P-fertilizers were 5, 15, and 25 mg kg-1 respectively. Experimental treatments were: T1 (control) = Without P; T2 = T1+RP (Rock Phosphate); T3 = T1+SSP (Simple superphosphate) and T4 = T1+TSP (Triple superphosphate). The results revealed that Cd concentration in T1 was 0.10, in T2 was 0.13, in T3 was 0.25 and in T4 was 0.29 mg kg-1. Thus, TSP caused maximum, and RP caused the least Cd accumulation in potato tubers (p <0.01). According to the obtained results, it can be concluded that application of P-fertilizers should be based on the soil test results and using rock phosphate and simple superphosphate should become a priority, we have to select P-fertilizers which has the lowest Cd concentration, and to take care of controlling the quality of imported P-fertilizers especially their Cd content.
马铃薯(Solanum tuberosum L.)是世界上继小麦、水稻和玉米之后的第四大重要农作物,因此提高其质量(污染物含量低)是非常重要和值得注意的。如果由于某种原因,土壤中的镉(Cd)浓度增加,马铃薯对镉的吸收就会增加。土壤中镉的主要来源之一是施用不平衡施肥,特别是大量消耗进口磷肥。为了研究不同磷肥来源对马铃薯块茎中Cd积累的影响,在伊朗Dehgolan的一个农场进行了试验。本实验采用随机完全区组设计(RCBD),共3个重复。试验开始前,在实验室采集土壤和磷肥样品并进行分析。土壤有效磷、锌、镉含量分别为8.0、0.75、0.15 mg kg-1,不同磷肥中Cd含量分别为5、15、25 mg kg-1。试验处理为:T1(对照)=无P;T2 = T1+RP(磷酸岩);T3 = T1+SSP(简单过磷酸钙)和T4 = T1+TSP(三重过磷酸钙)。结果表明:T1期Cd浓度为0.10,T2期为0.13,T3期为0.25,T4期为0.29 mg kg-1。因此,TSP对马铃薯块茎Cd积累的影响最大(p <0.01), RP对马铃薯块茎Cd积累的影响最小(p <0.01)。根据所得结果,磷肥的施用应以土壤试验结果为依据,优先使用磷矿磷和简单过磷酸钙,选择Cd浓度最低的磷肥,并注意控制进口磷肥的质量,特别是Cd含量。
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引用次数: 0
Expression Analysis of Mitogen-Activated Protein Kinases (MAPKs) Gene Family in Grapevine Berries 葡萄浆果中丝裂原活化蛋白激酶(MAPKs)基因家族表达分析
Pub Date : 2021-12-16 DOI: 10.26420/annagriccropsci.2021.1103
Hodaei A, W. S., Çakır B
Plants, as sessile living organisms, are dependent on signalling mechanisms. Mitogen-activated protein kinases (MAPKs) are a highly conserved gene family that take a role in switching an extracellular signal into an intercellular signal. Ripening-related processes in non-climacteric fruits are not as well understood as in climacteric fruits. In this regard, studying MAPKs in grape berries during developmental stages may lead to a better understanding of physiological interactions during commercially relevant stages, such as pigmentation, ripening, and phenolics accumulation in the berries. Each MAPK cascade involves three or four MAPK proteins that facilitate signal transduction by phosphorylation of downstream targets. We examined the relative expression of VvMAP2Ks and VvMAP4Ks in berries at two-weekly intervals, from flowering to over-ripening. Expression analysis of 5 MAP2Ks and 7 MAP4Ks suggested that both gene families may play an active role in development of berries. Expression of VvMAP2K1 showed a correlation with abscisic acid (ABA) and ethylene accumulation. Moreover, the expression pattern of VvMAP2K2 and VvMAP2K3 shows a correlation with auxin, and ABA accumulation respectively. Furthermore, VvMAP2K4 may have a role in berry size increment and halting stomatal development. In addition, VvMAP2K5 may play a role in floral organ development. VvMAP4Ks expression pattern moves them forward to be excellent markers for monitoring the effect of for instance climate changerelated stress on berry development.
植物作为无根的生物,依赖于信号机制。丝裂原活化蛋白激酶(MAPKs)是一个高度保守的基因家族,在细胞外信号转换为细胞间信号中起作用。非更年期果实的成熟相关过程不像更年期果实那样被很好地理解。在这方面,研究葡萄果实发育阶段的MAPKs可能有助于更好地理解在商业相关阶段的生理相互作用,如色素沉着、成熟和果实中酚类物质的积累。每个MAPK级联涉及三个或四个MAPK蛋白,通过磷酸化下游靶标促进信号转导。我们每隔两周检测一次浆果中vvmap2k和VvMAP4Ks的相对表达,从开花到过熟。5个MAP2Ks和7个MAP4Ks基因的表达分析表明,这两个基因家族可能在浆果发育中发挥积极作用。VvMAP2K1的表达与脱落酸(ABA)和乙烯积累相关。此外,VvMAP2K2和VvMAP2K3的表达模式分别与生长素和ABA积累相关。此外,VvMAP2K4可能在浆果大小增加和气孔发育中起抑制作用。此外,VvMAP2K5可能在花器官发育中发挥作用。VvMAP4Ks的表达模式使它们成为监测气候变化相关胁迫对浆果发育影响的优秀标记。
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引用次数: 0
Oleuropein Effectively Improves the Quality of Pig Sperm at 17o C 橄榄苦苷在170℃下有效提高猪精子质量
Pub Date : 2021-12-07 DOI: 10.26420/annagriccropsci.2021.1102
L. D., Z. W, L. K, Tian X, Y. G, Y. T.
Artificial Insemination (AI) has been widely used in pig reproduction because of its low price and high efficiency. Cryopreservation of sperm not only leads to a sharp decline in sperm motility, but had high price in pig reproduction. Therefore, most of farms stored pig sperm at 17°C. During the preservation of sperm, the accumulation of Reactive Oxygen (ROS) is the main reason for the decline of sperm motility. The aim of our study was to reduce the accumulation of ROS and improve sperm quality by adding oleuropein (OLE, CAS No. 32619- 42-4) during the storage. The sperm samples were collected and diluted with different concentrations of oleuropein (0, 12, 24, 36, 48, 60 μmol/L). Sperm motility, plasma membrane integrity, acrosome integrity, sperm Total Antioxidant Capacity (T-AOC), Malondialdehyde (MDA) content, Catalase (CAT) activity and Reactive Oxygen Species (ROS) content of boar sperm were measured. The results suggested that adding 36μM of OLE samples significantly improved sperm quality during preservation at 17o C.
人工授精技术以其价格低廉、效率高的特点在猪的繁殖中得到了广泛的应用。精子低温保存不仅会导致精子活力急剧下降,而且在猪的繁殖中代价高昂。因此,大多数农场将猪精子储存在17°C。在精子保存过程中,活性氧(Reactive Oxygen, ROS)的积累是精子活力下降的主要原因。我们的研究目的是通过在储存过程中添加橄榄苦苷(OLE, CAS No. 32619- 42-4)来减少ROS的积累,提高精子质量。采集精子样品,分别用不同浓度的橄榄苦苷(0、12、24、36、48、60 μmol/L)稀释。测定猪精子活力、质膜完整性、顶体完整性、精子总抗氧化能力(T-AOC)、丙二醛(MDA)含量、过氧化氢酶(CAT)活性和活性氧(ROS)含量。结果表明,添加36μM的OLE样品可显著提高精子在170℃保存期间的质量。
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引用次数: 0
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Annals of Agricultural & Crop Sciences
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