Zienab Abdullah, S. Helmy, Hanaa G. Elserougy, A. Abbas, Gehan A. Alwakeel
{"title":"Cardioprotective effect of Angiotensin (1-7) on myocardial infarction: Possible role of Nitric oxide and prostaglandins.","authors":"Zienab Abdullah, S. Helmy, Hanaa G. Elserougy, A. Abbas, Gehan A. Alwakeel","doi":"10.21608/BESPS.2018.8217","DOIUrl":null,"url":null,"abstract":"Objective: to detect the possible cardioprotective effect of Ang-(1-7) in rat model of MI; Also, possible role of NO and PGs in this probable cardioprotective effect of Ang- (1-7) was studied. Methods: Rats were divided randomly into 6 groups (8 rats each): Group I : Control group:, Group II: rats received S.C isoprenaline at a dose of 150 mg/kg/day on two consecutive days with an interval of 24 hours between applications, Group III: rats received Ang (1-7) (576μg/kg/day) S.C for 6 days after induction of MI ,Group IV: in which rats received Ang (1-7) (576μg/kg/day)+ L-NAME in the drinking water (80 mg/l) for 6days after induction of MI ,Group V: in which rats received Ang (1-7) (576μg/kg/day) +Indomethacin 5 mg/kg/day IP for 6 days after induction of MI ,Group VI: in which rats received both Ang-(1-7)+ L- NAME and Indomethacinat dose mentioned previously. Biochemical, histopathologial , and ECG changes were studied \nResults: ISO –MI group exhibited a significant rise in serum cardiac enzymes and disturbed lipid profile, increased myocardial damage score and Caspase3 expression when it is compared to the normal group (p<0.001). ECG changes of rat revealed elevation ST segment, QT interval prolongation, decrease QRS duration and voltage, and accelerated heart rate. Ang-(1-7) caused significant improvement in the studied parameters ,while co-infusion of L-NAME and or indomethacin prevent this effect of Ang-(1-7) .Combined L-NAME and indomethacin produce more deleterious effect than separate administration of them. Conclusion: Ang-(1-7) is considered one of the cardioprotective components of RAS .NO and PGs mediate the action of Ang-(1-7) and they may have an additive effect.","PeriodicalId":9347,"journal":{"name":"Bulletin of Egyptian Society for Physiological Sciences","volume":"39 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Egyptian Society for Physiological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/BESPS.2018.8217","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: to detect the possible cardioprotective effect of Ang-(1-7) in rat model of MI; Also, possible role of NO and PGs in this probable cardioprotective effect of Ang- (1-7) was studied. Methods: Rats were divided randomly into 6 groups (8 rats each): Group I : Control group:, Group II: rats received S.C isoprenaline at a dose of 150 mg/kg/day on two consecutive days with an interval of 24 hours between applications, Group III: rats received Ang (1-7) (576μg/kg/day) S.C for 6 days after induction of MI ,Group IV: in which rats received Ang (1-7) (576μg/kg/day)+ L-NAME in the drinking water (80 mg/l) for 6days after induction of MI ,Group V: in which rats received Ang (1-7) (576μg/kg/day) +Indomethacin 5 mg/kg/day IP for 6 days after induction of MI ,Group VI: in which rats received both Ang-(1-7)+ L- NAME and Indomethacinat dose mentioned previously. Biochemical, histopathologial , and ECG changes were studied
Results: ISO –MI group exhibited a significant rise in serum cardiac enzymes and disturbed lipid profile, increased myocardial damage score and Caspase3 expression when it is compared to the normal group (p<0.001). ECG changes of rat revealed elevation ST segment, QT interval prolongation, decrease QRS duration and voltage, and accelerated heart rate. Ang-(1-7) caused significant improvement in the studied parameters ,while co-infusion of L-NAME and or indomethacin prevent this effect of Ang-(1-7) .Combined L-NAME and indomethacin produce more deleterious effect than separate administration of them. Conclusion: Ang-(1-7) is considered one of the cardioprotective components of RAS .NO and PGs mediate the action of Ang-(1-7) and they may have an additive effect.