From Arabidopsis to rice genomics: a survey of French programmes

Michel Delseny , Georges Pelletier
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Abstract

During the last ten years, Arabidopsis thaliana has become the most favoured plant system for the study of many aspects of development and adaptation to adverse conditions and diseases. The sequencing of the Arabidopsis thaliana genome is nearly completed with more than 90 % of the sequence being released in public databases. This is the first plant genome to be analysed and it has revealed a tremendous amount of information about the nature of the genes it contains and its largely duplicated organisation. French groups have been involved in Arabidopsis genomics at several steps: EST (expressed sequence tags) sequencing, construction and ordering (physical mapping of chromosomes) of a YAC (yeast artificial chromosomes) library, genomic sequencing. In parallel an extensive programme of functional genomics is being undertaken through the systematic analysis of insertional mutants. This information provides a support for analysing other more economically important plant genomes such as the rice genome and constitutes the beginning of a systematic investigation on plant gene functions and will promote new strategies for plant improvement.

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从拟南芥到水稻基因组学:法国项目调查
在过去的十年中,拟南芥已成为最受青睐的植物系统,用于研究许多方面的发育和适应不利条件和疾病。拟南芥基因组的测序已接近完成,超过90%的序列已在公共数据库中发布。这是第一个被分析的植物基因组,它揭示了大量关于其包含的基因性质及其大量复制组织的信息。法国研究小组参与了拟南芥基因组学的几个步骤:EST(表达序列标签)测序,YAC(酵母人工染色体)文库的构建和排序(染色体的物理映射),基因组测序。与此同时,通过对插入突变体的系统分析,一个广泛的功能基因组学项目正在进行。这一信息为分析其他更具经济意义的植物基因组(如水稻基因组)提供了支持,并构成了对植物基因功能系统研究的开端,并将促进植物改良的新策略。
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