{"title":"Substance in Clostridium botulinum type A causing uterine contraction in rats","authors":"S. Sato, T. Kawata","doi":"10.3412/jsb.24.189","DOIUrl":null,"url":null,"abstract":"A substance stimulating contraction of the isolated rat uterus in Tyrode solution was found both in the culture supernatant and in the soluble cellular fractions of Clostridium botulinum type A by the Magnus method. The contractile substance was separated from the culture supernatant and partially purified by Dowex column chromatography and paper chromatography.This substance had a molecular weight lower than 180 and gave a pink spot (Rf value, 0.13) on the paper chromatogram run in butanol: acetic acid: water (3:1:1) by ninhydrin. It exhibited no positive reaction with diazo, Dragendorff, or Sakaguchi reagent.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"42 1","pages":"189-194"},"PeriodicalIF":0.0000,"publicationDate":"1969-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of bacteriology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3412/jsb.24.189","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A substance stimulating contraction of the isolated rat uterus in Tyrode solution was found both in the culture supernatant and in the soluble cellular fractions of Clostridium botulinum type A by the Magnus method. The contractile substance was separated from the culture supernatant and partially purified by Dowex column chromatography and paper chromatography.This substance had a molecular weight lower than 180 and gave a pink spot (Rf value, 0.13) on the paper chromatogram run in butanol: acetic acid: water (3:1:1) by ninhydrin. It exhibited no positive reaction with diazo, Dragendorff, or Sakaguchi reagent.
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