Application and composition of sawdust, grass straw, rice husk, and rice bran for white oyster mushroom (Pleurotus ostreatus Jacq.) growth media using hydrated lime sterilization

A. Hendrika, A. Soffan, M. Althaf, S. Amanda, R. Fani, P. Tampubolon, R. Lestari
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Abstract

The Oyster mushroom (Pleurotus ostreatus Jacq.) is one of the mushrooms that is easily cultivated. Most Indonesian mushroom farmers are using sawdust as a base material for growth media of oyster mushroom. In an effort to prevent decrease in the availability of sawdust, alternative base materials are needed to reduce the cost. This research aims to determine the mycelium growth on every tested substrate and to know the effect of rice bran addition in oyster mushroom’s mycelium growth rate. This research used sawdust, grass straw, and rice husk as the substrates. The effect of the presence of rice bran was done by adding rice bran to the substrate. This research used treatment composition as follows: M1 (sawdust 800 g and rice bran 204 g), M2 (sawdust 800 g), M3 (grass straw 400 g and rice bran 204 g), M4 (grass straw 400 g), M5 (rice husk 800 g and rice bran 204 g), and M6 (rice husk 800 g). The results showed that M2, M4, and M6 have mycelium 44.37 %, 28.54 % and 24.58 % respectively in 21 DAI. Our results indicate that we can use sawdust without adding rice bran as the base material to reduce the cost or substitute the sawdust with grass straw and without added rice bran to be an alternative base material for growth media.
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木屑、稻草、稻壳、米糠在白平菇(Pleurotus ostreatus Jacq.)水合石灰灭菌培养基中的应用及组成
平菇(Pleurotus ostreatus Jacq.)是一种容易栽培的蘑菇。大多数印尼蘑菇种植者使用锯末作为平菇生长培养基的基础材料。为了防止锯末可用性的减少,需要替代基础材料来降低成本。本研究旨在测定各试验基质上菌丝体的生长情况,了解添加米糠对平菇菌丝体生长速率的影响。本研究以木屑、草秆、稻壳为底物。通过在底物中加入米糠来考察米糠存在的影响。本研究采用的处理组合为:M1(锯末800 g、米糠204 g)、M2(锯末800 g)、M3(稻草400 g、米糠204 g)、M4(稻草400 g)、M5(稻壳800 g、米糠204 g)、M6(稻壳800 g)。结果表明,在21个DAI中,M2、M4和M6的菌丝率分别为44.37%、28.54%和24.58%。研究结果表明,可以采用不添加米糠的木屑作为基材,以降低成本,也可以用不添加米糠的稻草代替木屑作为生长培养基的替代基材。
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