A Comparison of Triploid Induction Validation Techniques

Abstract

Abstract Triploidy induction is a technique that allows genetic manipulation of chromosome number to control reproduction and potentially create faster-growing animals; however, most methods for inducing polyploidy are not 100% effective. Using sunshine bass (white bass Morone chrysops ♀ × striped bass M. saxatilis ♂) as a model, we cross-validated the most common verification techniques: DNA staining and fluorescence quantification with a flow cytometer, erythrocyte nuclear volume with a Coulter counter particle size analyzer, silver staining of nucleolar organizer regions (NORs), and cytological karyotyping. Results indicated that the electronic techniques of particle size analysis and flow cytometry were the simplest and quickest methods of validation. The major drawback of both electronic ploidy determination methods is the cost of the equipment required for analysis. Cytological karyotyping was the most accurate method for determining polyploidy because actual chromosome numbers were determined. It w...