Acute toxicity of drilling muds on Litopenaeus vannamei (Boone, 1931) postlarvae

G. León, Simón Andrés Rodríguez Satizábal, C. Romero, A. Herrera, Marlon Serrano Gómez
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引用次数: 3

Abstract

espanolL a toxicidad aguda de seis lodos de perforacion (base agua y base sintetico) fue evaluada utilizando postlarvas de Litopenaeus vannamei con la finalidad de establecer la concentracion letal media a 96 h (CL50). La Fase Suspendida Particulada (FSP) de cada lodo fue usada como material de prueba. Cada FSP de los lodos fue evaluada usando cinco concentraciones de prueba con cuatro replicas de cada una, incluido el control. Los valores de CL50 obtenidos de la FSP de los Lodos Base Agua (LBA) se encontraron entre 4224 ppm y 26635 ppm y entre 40781 ppm y 308248 ppm para los Lodos Base Sintetica (LBS). El protocolo de ensayo para toxicidad aguda con esta especie se valido mediante una carta control con el toxico de referencia (dicromato de potasio). Litopenaeus vannamei mostro tener las condiciones requeridas para ser usado en pruebas de toxicidad aguda. Este trabajo es pionero en Colombia para evaluar los efectos de los lodos de perforacion con un organismo marino local. portuguesA toxicidade aguda de seis lamas de perfuracao (base agua e base sintetica) foi avaliada utilizando postlarvas de Litopenaeus vannamei com a finalidade de estabelecer a concentracao letal media a 96 h (CL50). A Fase Suspensa Particulada (FSP) de cada lama foi usada como material de prova. Cada FSP das lamas foi avaliada usando cinco concentracoes de prova com quatro replicas de cada uma, incluido o controle. Os valores de CL50 obtidos da FSP das Lamas Base Agua (LBA) estavam entre 4224 ppm e 26635 ppm e entre 40781 ppm e 308248 ppm para as Lamas Base Sintetica (LBS). O protocolo de ensaio para toxicidade aguda com esta especie foi validada mediante uma carta controle com o toxico de referencia (dicromato de potassio). Litopenaeus vannamei mostrou ter as condicoes necessarias para ser usado em provas de toxicidade aguda. Este trabalho e pioneiro na Colombia para avaliar os efeitos das lamas de perfuracao com um organismo marinho local. EnglishA cute toxicity of six drilling muds (water based and synthetic based) was evaluated using Litopenaeus vannamei postlarvae to establish the 96 h median lethal concentration (LC50). The Suspended Particulate Phase (SPP) of each drilling mud was used as test material. Each mud SPP was evaluated using five test concentrations, and four replicates of each were used including the control. LC50 values obtained ranged from 4224 ppm to 26635 ppm for Water Based Mud (WBM) and from 40781 ppm to 308248 ppm for Synthetic Based Mud (SBM). The test protocol for acute toxicity for this species was validated through a control chart with the reference toxicant potassium dichromate. Litopenaeus vannamei clearly showed the required conditions to be used in acute toxicity tests. This research is pioneer in Colombia to determine the effects of drilling muds using a local marine organism.
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钻井泥浆对凡纳滨对虾(Boone, 1931)幼虫的急性毒性
以凡纳滨对虾(Litopenaeus vanannamei)后幼虫为研究对象,对6种钻井泥浆(水基和合成基)进行了西班牙语急性毒性评价,确定了96 h的平均致死浓度(lc50)。在本研究中,我们分析了不同污泥类型的颗粒悬浮相(FSP)。在本研究中,我们评估了两种不同浓度的FSP污泥,每一种浓度都有4个重复,包括对照。水基污泥(LBA)的spf值在4224 ppm ~ 26635 ppm之间,合成基污泥(LBS)的spf值在40781 ppm ~ 308248 ppm之间。对该物种的急性毒性试验方案通过参考毒物(重铬酸钾)的对照图进行验证。凡纳滨对虾已证明具有用于急性毒性试验所需的条件。这项工作是哥伦比亚评估钻井泥浆对当地海洋生物影响的开创性工作。摘要以凡纳滨对虾后幼虫为研究对象,对6种葡萄牙钻井泥浆(水基和合成基)的急性毒性进行了评价,确定了96 h时的平均致死浓度(lc50)。以各污泥的悬浮颗粒相(spf)为试验材料。每个FSP污泥使用5个试验浓缩物进行评估,每个浓缩物有4个重复,包括对照。水基污泥(LBA)的lc50值在4224 ppm ~ 26635 ppm之间,合成污泥(LBS)的lc50值在40781 ppm ~ 308248 ppm之间。该物种的急性毒性试验方案通过对照图验证了参考毒性(重铬酸钾)。凡纳滨对虾已被证明具有用于急性毒性试验的条件。这项工作是哥伦比亚评估钻井泥浆对当地海洋生物影响的开创性工作。用凡纳滨对虾幼虫后测定了六种钻井泥浆(水基和合成基)的cute毒性,以确定96 h中致死浓度(LC50)。= =地理= =根据美国人口普查,这个县的总面积为,其中土地和(2.641平方公里)水。采用5种试验浓度对每种泥浆进行评价,并使用每种泥浆的4个重复物,包括对照物。= =地理= =根据美国人口普查,该镇的总面积为,其中土地和(1.2%)水。该物种急性毒性试验方案已通过对照图与参考毒物重铬酸钾进行验证。凡纳滨对虾清楚地说明了用于急性毒性试验的要求条件。这项研究是哥伦比亚利用当地海洋生物确定钻井泥浆效果的先驱。
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