In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus, Colletotrichum destructivum

T. Vasić, Uroš D. Vojinović, Suzana Žujović, V. Krnjaja, S. Živkovic, J. Marković, M. Stević
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引用次数: 2

Abstract

Abstract Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900 bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC50=0.39 µg mL−1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC50=0.61 µg mL−1), followed by azoxystrobin (mean EC50=2.83 µg mL−1) and flutriafol (mean EC50=2.11 µg mL−1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC50=35.31 and 62.83 µg mL−1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC50=0.49 and 0.19 µg mL−1, respectively), while the rest of isolates were highly resistant.
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不同作用方式杀菌剂对紫花苜蓿炭疽病真菌的体外毒力研究
摘要研究了从塞尔维亚苜蓿中采集的24株炭疽菌(Colletotrichum destructivum O’gara)对8种选定杀菌剂的敏感性。并对分离株进行了分子鉴定和致病性试验。采用菌丝生长测定法体外评价杀菌剂的敏感性。所有分离株均表现出显著的致病性,接种2天后引起苜蓿幼苗根尖坏死。利用引物对GSF1-SR1,并将扩增片段与标记物(M)进行比较,确定所有分离株均存在预期大小约900 bp的扩增子。本研究检测的菌株对体外杀菌剂的敏感性不同。QoI(醌外源抑制剂)杀菌剂吡唑菌酯(平均EC50=0.39 μ g mL - 1)和DMI(去甲基化抑制)杀菌剂戊康唑(平均EC50=0.61 μ g mL - 1)对菌丝生长有高度抑制作用,其次是偶氮菌酯(平均EC50=2.83 μ g mL - 1)和氟triafol(平均EC50=2.11 μ g mL - 1)。多位点杀菌剂百菌清和MBC(甲基苯并咪唑氨基甲酸酯)杀菌剂噻吩-甲基具有中等抑制作用,平均EC50分别为35.31和62.83µg mL−1。13株菌株对SDHI(琥珀酸脱氢酶抑制剂)杀菌剂boscalid和fluxapyroxad敏感(平均EC50分别为0.49和0.19µg mL−1),其余菌株高度耐药。
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