Dirofilaria Repens in Dogs and Humans in Slovenia

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Onderstepoort Journal of Veterinary Research Pub Date : 2022-03-01 DOI:10.2478/jvetres-2022-0008
T. Kotnik, A. V. Rataj, B. Šoba
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引用次数: 1

Abstract

Abstract Introduction The prevalence of Dirofilaria repens in dogs in countries bordering Slovenia ranges from 1.5% to 47.3%. The aim of this study was to estimate its prevalence in Slovenian dogs and to present the cases of dirofilariasis diagnosed in humans from 2010 to 2020. Material and Methods Epidemiological data were collected and blood samples were taken from 465 dogs older than one year and born in Slovenia. A real-time PCR was performed on all samples to detect filarioid DNA, and a D. repens-and D. immitis-specific real-time PCR was performed on positive samples. Blood samples from 446 dogs were tested for Dirofilaria spp. using a modified Knott’s test. Human cases were diagnosed from histological sections of excised subcutaneous nodules. Descriptive statistics were used to characterise the samples. The one-sample nonparametric chi-squared test was used to assess whether categories of a variable were equally distributed. Results Three dogs’ samples tested positive for D. repens using the species-specific real-time PCR, while D. immitis DNA was not detected. The modified Knott’s test was positive in two of the three PCR-positive dogs, two of which had never travelled outside Slovenia’s borders. Four human patients with D. repens dirofilariasis were diagnosed. Since their travel history was unknown, autochthonous transmission could not be confirmed. Conclusion Our study demonstrated a 0.64% prevalence of D. repens infection in dogs in Slovenia. Two cases could be autochthonous.
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斯洛维尼亚的狗和人出现了Dirofilaria
在与斯洛文尼亚接壤的国家,犬类中Dirofilaria repens的流行率为1.5%至47.3%。本研究的目的是估计其在斯洛文尼亚狗中的患病率,并介绍2010年至2020年在人类中诊断出的dirofil虫病病例。材料与方法收集了465只出生在斯洛文尼亚的一岁以上的狗的流行病学数据和血液样本。对所有样品进行实时PCR检测丝虫病样DNA,对阳性样品进行D. repens和D. immitis特异性实时PCR检测。对446只狗的血液样本进行了Dirofilaria sps检测,使用了一种改进的Knott检测方法。人类病例是通过切除的皮下结节的组织学切片诊断的。使用描述性统计来描述样本的特征。使用单样本非参数卡方检验来评估变量的类别是否均匀分布。结果3只犬标本经实时荧光定量PCR检测呈鼠棘球蚴阳性,未检出犬棘球蚴DNA。修改后的诺特氏试验在三只pcr阳性的狗中有两只呈阳性,其中两只从未到过斯洛文尼亚境外。诊断出4例人雷氏迪罗氏丝虫病。由于他们的旅行史未知,因此无法确认本地传播。结论:我们的研究表明斯洛文尼亚犬中repens感染率为0.64%。两种情况可能是先天性的。
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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
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