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Factors associated with the rabies vaccination status of dogs in households in Beni City, D.R. Congo. 与刚果(金)贝尼市家庭中狗的狂犬病疫苗接种情况有关的因素。
IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-08-16 DOI: 10.4102/ojvr.v91i2.2168
Prince D Kimpanga, Emery K Taghembwa, Gauthier M Mubenga, Jean-Marie T Makwera, Norbert M Muhongya, Odrade B Chabikuli, Justin M Masumu

Human rabies transmitted by dogs still kills thousands of people each year worldwide. Dog bites are common in the city of Beni (Democratic Republic of Congo), which shows low rabies vaccination coverage. This study aimed to determine the factors associated with the rabies vaccination status of dogs. A cross-sectional analytical study was conducted in the town of Beni among dog owners, during a household survey selected using a multistage sampling. The information sought concerned the knowledge and characteristics of the dog owners as well as the vaccination status of these dogs. Logistic regression was used to investigate associations between the vaccination status of the dogs and the main independent factors. Rabies vaccination coverage in Beni was 26% (95% confidence interval [CI]: 22% - 30%). The main factors associated with the rabies vaccination status of the dog were primary education level of household head (adjusted odds ratio [aOR]:4.8; 95% CI: 1.2- 19.8); university education level of household head (aOR: 5.9; 95% CI: 1.6-22); perceived rabies severity (aOR: 44. 4; 95% CI: 10.4-188), having more than one dog in the household (aOR: 2.6; 95% CI: 1.6-4.3); age range 7-12 months (aOR: 0.2; 95% CI: 0.1-0.6) and confined dog breeding (aOR: 3.9; 95% CI: 1.1-14.9). The low vaccination coverage in Beni requires mass vaccination campaigns against canine rabies targeting the dog owners with low education levels, those raising more than one dog, with stray dogs or dogs less than 12 months old.

全世界每年仍有数千人死于由狗传播的人类狂犬病。被狗咬伤在贝尼市(刚果民主共和国)很常见,而该市的狂犬病疫苗接种率却很低。本研究旨在确定与狗狂犬病疫苗接种状况相关的因素。在贝尼镇对养狗者进行了一次横断面分析研究,调查采用多阶段抽样的方式进行。调查信息涉及狗主人的知识和特征以及这些狗的疫苗接种情况。采用逻辑回归法调查了狗的疫苗接种情况与主要独立因素之间的关联。贝尼的狂犬病疫苗接种率为 26%(95% 置信区间 [CI]:22% - 30%)。与狗的狂犬病疫苗接种状况相关的主要因素是户主的初等教育水平(调整后的几率比 [aOR]:4.8;95% CI:1.2- 19.8);户主的大学教育水平(aOR:5.9;95% CI:1.4;95% CI:10.4-188)、家中有一只以上的狗(aOR:2.6;95% CI:1.6-4.3)、年龄范围为 7-12 个月(aOR:0.2;95% CI:0.1-0.6)和圈养狗(aOR:3.9;95% CI:1.1-14.9)。贝尼地区狂犬病疫苗接种覆盖率较低,因此需要针对教育水平较低、饲养不止一只狗、饲养流浪狗或狗龄不足 12 个月的狗主人开展大规模狂犬病疫苗接种活动。
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引用次数: 0
Perceptions of ethnoveterinary medicine among animal healthcare practitioners in South Africa. 南非动物保健从业人员对民族兽医学的看法。
IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-07-31 DOI: 10.4102/ojvr.v91i1.2138
Rendani V Ndou, Simeon A Materechera, Mulunda Mwanza, Wilfred Otang-Mbeng

The South African government recognises the value of traditional healing and supports an integrated healthcare system. In the veterinary field, successful integration depends on the perceptions of different animal healthcare practitioners (AHCPs). This study aimed to determine the perceptions of ethnoveterinary medicine among state-employed veterinarians and animal health technicians in the North West province. A mixed model questionnaire and non-probability sampling gathered quantitative and qualitative data from 53 participants. Statistical and thematic analysis processes were applied to quantitative and qualitative data, respectively. Overall, 77.4% of participants were aware of ethnoveterinary medicine practices. Approval of the use of ethnoveterinary medicine by farmers was above-average at a mean of 3.57, standard deviation (s.d.) = 1.016 on a 5-point Likert scale. However, 65.9% doubted the effectiveness of ethnoveterinary medicine, which suggests tolerance but no appreciation of its value. The Chi-square test revealed a significant relationship between district municipality of employment and awareness at χ2 = 17.490, df = 3, p = 0.001. Thematic analysis of comments showed that some participants perceived ethnoveterinary medicine as unscientific and interfering with Western veterinary medicine, while some considered it a good complement to Western veterinary medicine. The study also found that 20.8% of AHCPs use ethnoveterinary medicine to treat certain diseases. In conclusion, the lack of appreciation for ethnoveterinary medicine among AHCPs may hinder government-proposed integration, emphasising the need for programmes to improve perceptions among these stakeholders.Contribution: This is first such study to report on perceptions of ethnoveterinary medicine among AHCPs, and assess their readiness for an integrated animal health system.

南非政府承认传统疗法的价值,并支持整合医疗保健系统。在兽医领域,整合成功与否取决于不同动物保健从业人员(AHCPs)的看法。本研究旨在确定西北省国家聘用的兽医和动物保健技术人员对民族兽医的看法。研究采用混合模式问卷调查和非概率抽样法,从 53 名参与者那里收集了定量和定性数据。对定量和定性数据分别进行了统计和专题分析。总体而言,77.4%的参与者了解民族兽医学实践。在 5 点李克特量表中,农民对使用民族兽药的认可度高于平均水平,平均值为 3.57,标准差(s.d. )= 1.016。然而,65.9% 的人对民族兽医的有效性表示怀疑,这表明他们对民族兽医持宽容态度,但并不了解其价值。卡方检验表明,就业区市与认知度之间存在显著关系,χ2 = 17.490,df = 3,p = 0.001。对评论的专题分析表明,一些参与者认为民族兽医学不科学,干扰了西兽医学,而另一些人则认为它是西兽医学的良好补充。研究还发现,20.8% 的兽医师使用民族兽医学治疗某些疾病。总之,全科医生对民族兽医缺乏了解,这可能会阻碍政府提出的整合计划,因此有必要制定计划来改善这些利益相关者的看法:本研究首次报告了动物卫生保健人员对民族兽医学的看法,并评估了他们对一体化动物卫生系统的准备情况。
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引用次数: 0
Clinical Coxiella burnetii infection in sable and roan antelope in South Africa 南非紫貂羚和罗恩羚临床感染烧伤柯西氏菌的情况
IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-07-26 DOI: 10.4102/ojvr.v91i1.2151
Wikus Wiedeman, Akorfa B. Glover, J. Steyl, JH O’dell, H. van Heerden
Various zoonotic microorganisms cause reproductive problems such as abortions and stillbirths, leading to economic losses on farms, particularly within livestock. In South Africa, bovine brucellosis is endemic in cattle, and from 2013–2018, outbreaks of Brucella melitensis occurred in sable. Coxiella burnetii, the agent responsible for the zoonotic disease known as Q-fever and/or coxiellosis, also causes reproductive problems and infects multiple domestic animal species worldwide, including humans. However, little is known of this disease in wildlife. With the expansion of the wildlife industry in South Africa, diseases like brucellosis and coxiellosis can significantly impact herd breeding success because of challenges in identifying, managing and treating diseases in wildlife populations. This study investigated samples obtained from aborted sable and roan antelope, initially suspected to be brucellosis, from game farms in South Africa using serology tests and ruminant VetMAX™ polymerase chain reaction (PCR) abortion kit. The presence of C. burnetii was confirmed with PCR in a sable abortion case, while samples from both sable and roan were seropositive for C. burnetii indirect enzyme-linked immunosorbent assay (iELISA). This study represents the initial report of C. burnetii infection in sable and roan antelope in South Africa. Epidemiological investigations are crucial to assess the risk of C. burnetii in sable and roan populations, as well as wildlife and livestock in general, across South Africa. This is important in intensive farming practices, particularly as Q-fever, being a zoonotic disease, poses a particular threat to the health of veterinarians and farm workers as well as domestic animals.Contribution: A report of clinical C. burnetii infection in the wildlife industry contributes towards the limited knowledge of this zoonotic disease in South Africa.
各种人畜共患病微生物会导致流产和死胎等生殖问题,给农场造成经济损失,尤其是在牲畜中。在南非,牛布鲁氏杆菌病在牛群中流行,从 2013-2018 年开始,黑貂中爆发了布鲁氏杆菌病。被称为 Q 热和/或柯西氏菌病的人畜共患疾病的病原体柯西氏菌也会导致生殖问题,并在全球范围内感染多种家畜物种,包括人类。然而,人们对野生动物中的这种疾病知之甚少。随着南非野生动物产业的扩张,布鲁氏菌病和柯西氏菌病等疾病会严重影响畜群繁殖的成功率,因为在识别、管理和治疗野生动物疾病方面存在挑战。本研究使用血清学测试和反刍动物 VetMAX™ 聚合酶链反应 (PCR) 流产试剂盒对从南非猎场流产的紫貂羚羊和大羚羊身上获得的样本进行了调查,这些样本最初被怀疑患有布鲁氏菌病。在一例黑貂流产病例中,通过聚合酶链反应证实了布氏杆菌的存在,而黑貂和狍的样本在布氏杆菌间接酶联免疫吸附试验(iELISA)中的血清反应均呈阳性。这项研究是南非首次报告貂羚和狍子感染烧伤弧菌。流行病学调查对于评估烧伤弧菌在南非黑貂和大羚羊种群以及野生动物和家畜中的风险至关重要。这对集约化养殖非常重要,尤其是 Q 热作为一种人畜共患病,对兽医、农场工人和家畜的健康构成了特别的威胁:贡献:一份关于野生动物业中烧伤蜱临床感染的报告有助于人们了解南非这种人畜共患病的有限知识。
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引用次数: 0
Evaluation of commercial ELISA kits' diagnostic specificity for FAST diseases in wild animals. 评估商用酶联免疫吸附试剂盒对野生动物 FAST 疾病的诊断特异性。
IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Pub Date : 2024-06-28 DOI: 10.4102/ojvr.v91i1.2164
Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Jovan Mirčeta, Branislav Kureljušić, Milutin Đorđević, Nikola Vasković

Wild animals, sharing pathogens with domestic animals, play a crucial role in the epidemiology of infectious diseases. Sampling from wild animals poses significant challenges, yet it is vital for inclusion in disease surveillance and monitoring programmes. Often, mass surveillance involves serological screenings using enzyme-linked immunosorbent assay (ELISA) tests, typically validated only for domestic animals. This study assessed the diagnostic specificity of commercially available ELISA tests on 342 wild ruminant serum samples and 100 from wild boars. We evaluated three tests for foot-and-mouth disease: two for Peste des petits ruminants, two for Rift Valley fever and one for Capripox virus. Diagnostic specificity was calculated using the formula True Negative/(False Positive + True Negative). Cohen's kappa coefficient measured agreement between tests. Results showed high specificity and agreement across all tests. Specificity for foot-and-mouth disease (FMD) ranged from 93.89% for Prionics to 100% for IDEXX, with IDvet showing 99.6%. The highest agreement was between FMD IDvet and IDEXX at 97.1%. Rift Valley fever (RVF) tests, Ingezim and IDvet, achieved specificities of 100% and 98.83%, respectively. The optimal specificity was attained by retesting single reactors and inactivating the complement.Contribution: Commercially available ELISA kits are specific for foot-and-mouth disease and similar transboundary animal diseases and can be used for highly specific wild animal testing.

野生动物与家养动物共享病原体,在传染病的流行病学中发挥着至关重要的作用。对野生动物进行采样是一项重大挑战,但对于将其纳入疾病监测和监控计划却至关重要。通常情况下,大规模监测涉及使用酶联免疫吸附试验(ELISA)进行血清学筛查,这种试验通常只对家养动物有效。本研究评估了市售酶联免疫吸附试验对 342 份野生反刍动物血清样本和 100 份野猪血清样本的诊断特异性。我们评估了三种口蹄疫检测方法:两种用于检测小反刍兽疫,两种用于检测裂谷热,一种用于检测痘病毒。诊断特异性的计算公式为真阴性/(假阳性+真阴性)。科恩卡帕系数(Cohen's kappa coefficient)用于衡量检测之间的一致性。结果显示,所有检测项目的特异性和一致性都很高。对口蹄疫(FMD)的特异性从 Prionics 的 93.89% 到 IDEXX 的 100% 不等,其中 IDvet 为 99.6%。口蹄疫 IDvet 与 IDEXX 的一致性最高,达到 97.1%。Ingezim和IDvet的裂谷热(RVF)检测特异性分别为100%和98.83%。通过重测单个反应物和灭活补体,可达到最佳特异性:贡献:市售酶联免疫吸附试剂盒对口蹄疫和类似的跨境动物疾病具有特异性,可用于高特异性野生动物检测。
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引用次数: 0
An investigation into Toxoplasma gondii at the human-livestock-wildlife interface, South Africa 对南非人类-牲畜-野生动物交界处弓形虫的调查
IF 2.7 3区 农林科学 Q1 Veterinary Pub Date : 2024-04-18 DOI: 10.4102/ojvr.v91i1.2130
R. P. Bokaba, V. Dermauw, D. Morar-Leather, P. Dorny, Louis Van Schalkwyk, L. Neves
Interface areas shared by humans, domestic and wild animals may serve as high transmission contexts for Toxoplasma gondii. However, knowledge about the epidemiology of T. gondii in such areas is currently limited. The present study assessed the seroprevalence of T. gondii in different hosts from Mpumalanga, South Africa. Furthermore, we investigated the local knowledge and related practices about T. gondii by conducting a questionnaire study in the community. Blood samples were obtained and analysed for T. gondii antibodies using a commercial multispecies latex agglutination kit. The seroprevalence detected in humans (n = 160; patients showing signs of acute febrile illness), cats (n = 9), chickens (n = 336) and goats (n = 358) was 8.8%, 0.0%, 4.2% and 11.2%, respectively. Seroprevalence in impalas (n = 97), kudus (n = 55), wild dogs (n = 54), wildebeests (n = 43), warthogs (n = 97) and zebras (n = 68) was calculated at 5.2%, 7.3%, 100.0%, 20.9%, 13.4% and 9.1%, respectively. The questionnaire revealed that 63.0% of household owners were subsistence farmers, and 35.9% were pet owners. A high level of female participation was found (75.3%) when compared to male participation (24.7%). The results show a low circulation of T. gondii in the domestic cycle and suggest the presence of possible bridges between the wildlife cycle and the surrounding domestic cycle.Contribution: The study contributes to identifying transmission patterns and risk factors of T. gondii within human and animal populations. This topic fits within the scope of the journal presenting original research in veterinary science, with the focus on wild and domestic populations on the African continent on a topic of universal importance.
人类、家畜和野生动物共同生活的交界地区可能是弓形虫的高传播区。然而,目前对此类地区弓形虫流行病学的了解还很有限。本研究评估了弓形虫在南非姆普马兰加省不同宿主中的血清流行率。此外,我们还通过在社区开展问卷调查,了解当地人对淋病的认识和相关做法。我们采集了血液样本,并使用商用多物种乳胶凝集试剂盒分析了淋病双球菌抗体。在人类(n = 160;急性发热性疾病患者)、猫(n = 9)、鸡(n = 336)和山羊(n = 358)中检测到的血清流行率分别为 8.8%、0.0%、4.2% 和 11.2%。黑斑羚(n = 97)、疣猪(n = 55)、野狗(n = 54)、角马(n = 43)、疣猪(n = 97)和斑马(n = 68)的血清流行率分别为 5.2%、7.3%、100.0%、20.9%、13.4% 和 9.1%。调查问卷显示,63.0%的家庭主人是自给自足的农民,35.9%是宠物主人。与男性参与率(24.7%)相比,女性参与率较高(75.3%)。结果表明,淋病双球菌在家庭循环中的传播率较低,并表明野生动物循环与周边家庭循环之间可能存在桥梁:该研究有助于确定淋病双球菌在人类和动物群体中的传播模式和风险因素。该主题符合《兽医科学》杂志介绍兽医科学原创性研究的范围,重点关注非洲大陆的野生和家养人群,这是一个具有普遍意义的主题。
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引用次数: 0
Thermostability study of virulent Newcastle disease viruses isolated in Southern Angola 安哥拉南部分离出的毒性新城疫病毒的热稳定性研究
IF 2.7 3区 农林科学 Q1 Veterinary Pub Date : 2024-04-15 DOI: 10.4102/ojvr.v91i1.2147
António Neto, A. M. Henriques, T. Fagulha, M. Fevereiro
Newcastle disease (ND) is endemic in Angola. Several outbreaks of ND occurred in small backyard flocks and village chickens with high mortality in the southern provinces of the country, Cunene, Namibe and Huíla, in 2016 and 2018. In those years, 15 virulent ND virus (NDV) strains were isolated and grouped within subgenotype 2 of genotype VII (subgenotype VII.2). We now present a study on the thermostability of the isolates, aiming at the selection of the most thermostable strains that, after being genetically modified to reduce their virulence, can be adapted to the production of vaccines less dependent on cold chain and more adequate to protect native chickens against ND. Heat-inactivation kinetics of haemagglutinin (Ha) activity and infectivity (I) of the isolates were determined by incubating aliquots of virus at 56 °C for different time intervals. The two isolates from Namibe province showed a decrease in infectivity of 2 log10 in ≤ 10 min, therefore belonging to the I-phenotype, but while the NB1 isolate from 2016 maintained the Ha activity up to 30 min and was classified as thermostable virus (I−Ha+), the Ha activity of the 2018 NB2 isolate decreased by 2 log2 in 30 min, being classified as a thermolabile virus (I−Ha−). Of the 13 NDV isolates from Huíla province, 10 isolates were classified as thermostable, eight with phenotype I+Ha+ and 2 with phenotype I−Ha+. The other three isolates from this province were classified as thermolabile viruses (I−Ha−).Contribution: This study will contribute to the control and/or eradication of Newcastle disease virus in Angola. The thermostable viral strains isolated from chickens in the country can be genetically manipulated by reverse genetic technology in order to reduce their virulence and use them as a vaccine in the remote areas of Angola.
新城疫(ND)是安哥拉的地方病。2016年和2018年,在该国南部省份库内内、纳米贝和威拉,小型散养鸡群和村庄鸡群爆发了数次新城疫疫情,死亡率很高。在这两年中,分离出了 15 株毒性 ND 病毒(NDV),并将其归入基因型 VII 的亚基因型 2(亚基因型 VII.2)。我们现在对这些分离株的热稳定性进行研究,旨在筛选出热稳定性最强的毒株,这些毒株在经过基因改造以降低其毒力后,可用于生产对冷链依赖性较低的疫苗,从而更有效地保护本地鸡免受 ND 病毒的感染。通过将等分的病毒在 56 °C 下培养不同的时间间隔,测定了分离物血凝素(Ha)活性和感染性(I)的热灭活动力学。纳米贝省的两个分离株在≤10分钟内感染力下降了2 log10,因此属于I型病毒,但2016年的NB1分离株在30分钟内仍保持Ha活性,被归类为热稳定性病毒(I-Ha+),而2018年的NB2分离株在30分钟内Ha活性下降了2 log2,被归类为热稳定性病毒(I-Ha-)。在威拉省的 13 个 NDV 分离物中,10 个分离物被归类为热稳定性病毒,8 个表型为 I+Ha+ ,2 个表型为 I-Ha+。该省的另外 3 个分离株被归类为耐热病毒(I-Ha-):本研究将有助于控制和/或根除安哥拉的新城疫病毒。可通过反向遗传技术对从该国鸡群中分离出的恒温病毒株进行遗传操作,以降低其毒性,并将其用作安哥拉偏远地区的疫苗。
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引用次数: 0
Erratum: Ascofuranone antibiotic is a promising trypanocidal drug for nagana. 参考文献:Ascofuranone 抗生素是一种很有前景的纳加那锥虫杀灭药物。
IF 2.7 3区 农林科学 Q1 Veterinary Pub Date : 2024-03-22 DOI: 10.4102/ojvr.v91i1.2174
Keisuke Suganuma, Kennedy M Mochabo, Judith K Chemuliti, Kiyoshi Kita, Noboru Inoue, Shin-Ichiro Kawazu

No abstract available.

无摘要。
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引用次数: 0
Hepatoprotective effects of leaf extract of Annona senegalensis against aflatoxin B1 toxicity in rats 黑木耳叶提取物对黄曲霉毒素 B1 大鼠肝脏毒性的保护作用
IF 2.7 3区 农林科学 Q1 Veterinary Pub Date : 2024-03-11 DOI: 10.4102/ojvr.v91i1.2133
Rhulani Makhuvele, K. Foubert, N. Hermans, L. Pieters, Luc Verschaeve, E. Elgorashi
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引用次数: 0
Corrigendum: The outbreak of seasonal goat's brucellosis in the Swat ecosystem of Khyber Pakhtunkhwa. 更正:开伯尔-普赫图赫瓦省斯瓦特生态系统爆发季节性山羊布鲁氏菌病。
IF 2.7 3区 农林科学 Q1 Veterinary Pub Date : 2024-02-29 DOI: 10.4102/ojvr.v91i1.2150
Nabilla Qayum, Muhammad N Uddin, Wajid Khan, Habib Un Nabi, Taj- Ud- Din, Muhammad Suleman, Hanif Ur Rahman, Iftikhar Ali, Ahmed M Hassan, Rafa Almeer, Farman Ullah

No abstract available.

无摘要。
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引用次数: 0
Ascofuranone antibiotic is a promising trypanocidal drug for nagana Ascofuranone 抗生素是一种很有前景的纳加那锥虫杀灭药物
IF 2.7 3区 农林科学 Q1 Veterinary Pub Date : 2024-02-08 DOI: 10.4102/ojvr.v91i1.2115
Keisuke Suganuma, K. Mochabo, Judith K. Chemuliti, Kita Kiyoshi, Inoue Noboru, Shin-ichiro Kawazu
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引用次数: 0
期刊
Onderstepoort Journal of Veterinary Research
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