Method development and validation for simultaneous estimation of netupitant and palonosetron in bulk and pharmaceutical dosage form by using RP-HPLC

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Netupitant and Palanosetron in Tablet dosage form. Chromatogram was run through Std Discovery C18 250 x 4.6 mm, 5. Mobile phase containing Buffer 0.1% OPA (2.2ph): Acetonitrile taken in the ratio 55:45 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA. Temperature was maintained at 30°C. Optimized wavelength selected was 230 nm. Retention time of Netupitant and Palanosetron were found to be 2.325min and 3.026min. %RSD of the Netupitant and Palanosetron were and found to be 0.9 and 0.6 respectively. %Recovery was obtained as 100.28% and 99.76% for Netupitant and Palanosetron respectively. LOD, LOQ values obtained from regressionequations of Netupitant and Palanosetron were 1.01, 3.05 and 0.001, 0.003 respectively. Regression equation of Netupitant is y = 13900x + 26511, and y = 563617x + 1740.8 of Palanosetron. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.