EFFECT OF METRONIDAZOLE ON ADHERENCE PROPERTY OF GARDNERELLA VAGINALIS ISOLATED FROM PRETERM LABOR PATIENTS IN AL-HILLA CITY, IRAQ

Abstract

One hundred fifty clinical sample were collected from preterm labor patients with (bacterial vaginosis, urinary tract infection and aborted women) 80, 15, 55 respectively, admitted to Babylon Maternity and pediatric hospital and Al-Hilla Teaching Hospital, at the period from February to October 2016. These high vaginal samples were subjected to different methods of identification of G.vginalis mainly traditional bacteriological methods. It was found that 6 (4%) G.vaginalis isolates were recovered by using selective media and Viteck 2 system where 30 (20%) isolates were recovered dependent on direct extraction to high vaginal swab on molecular level. The screening of the adherence property were carried out to all (6) isolates and showed that all of it were capable to adhere on the epithelial cell then subjected to Metranidazole reduce it. Finally in vitro anti-adherence activity to Metronidazole were studied and the results revealed that all isolates were inhibited by aqueous solution at concentration (5%) which gave results (100%) very effective to eradication adherence, the results proved that is effective on disrupted adherence in the first step.