Isolation of a new Aspergillus iranicus strain from the soil of southwestern Algeria exhibiting potential palm date-cellulose degrading activity

S. Makhloufi, A. Makhloufi, Y. Chebloune
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Abstract

Microorganisms with biotechnological properties are needed for transformation or degradation of biological products. Here we isolated a new Aspergillus strain (MS-34) from the soil of southwestern Algeria and found that it is related to Aspergillus iranicus. The MS-34 was examined for possible biotechnological properties and we found it to be highly efficacious at degrading palm date cellulose following fermentation. Pretreatment fermentation of palm in 50 % generated the highest (197.84 mg/L) carbohydrate concentration. Pretreatment with 20% KOH produced a maximum 112.97 IU/ml and 47.62 IU/ml for endoglucanase and exoglucanase activities respectively. Besides, glucose and proteins concentration in batch fermentations with 1% CMC medium fluctuated between 86.41 mg/l to 108.85 mg/l and the protein level was 0.713- 0.991 mg/ml. There were significant temperature and pH effects on the enzymatic activity and the optimal temperature conditions were found to be 50-60°C and pH 4.0. Since palm dates are one of the massive agricultural products of the Southeastern and Southwestern Algeria, use of this strain for transformation of palm tree waste will be an advanced biotechnology tool that will contribute to significantly reduce pollution linked to waste incineration. In addition, resulting fermentation products may be used as sources of energy.
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从阿尔及利亚西南部土壤中分离出一株新的伊朗曲霉菌株,显示出潜在的棕榈枣纤维素降解活性
生物制品的转化或降解需要具有生物技术特性的微生物。本文从阿尔及利亚西南部土壤中分离到一株新的曲霉(MS-34),发现它与伊朗曲霉有亲缘关系。研究了MS-34可能的生物技术特性,我们发现它在发酵后降解棕榈枣纤维素方面非常有效。50%预处理发酵的棕榈碳水化合物浓度最高(197.84 mg/L)。20% KOH预处理后,内切葡聚糖酶和外切葡聚糖酶活性最高分别为112.97 IU/ml和47.62 IU/ml。在1% CMC培养基中间歇发酵,葡萄糖和蛋白质浓度在86.41 ~ 108.85 mg/l之间波动,蛋白质水平在0.713 ~ 0.991 mg/ml之间波动。温度和pH值对酶活性有显著影响,最佳温度条件为50 ~ 60℃,pH值为4.0。由于棕榈枣是阿尔及利亚东南部和西南部的大量农产品之一,使用这种菌株转化棕榈树废物将是一种先进的生物技术工具,将有助于大大减少与废物焚烧有关的污染。此外,产生的发酵产物可以用作能源。
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