Optimizing Germination of Melia volkensii Gürke after Storage of Seeds and Nuts in Different Storage Conditions

Njehu Jm, Wabuyele Emily, Mutune An, Kevin Omollo, Kamondo Bm
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Abstract

This study was conducted to understand the effect of nature of seed (nut or seed), storage conditions and storage time on the germination of Melia volkensii seeds. Two seed lots were stored for germination tests after storage of germination materials for 2, 4 and 6 months. Laboratory experimental setup involved three treatments: 1) some seeds were extracted while others were stored as nuts, 2) Storing the seeds in open and closed containers and 3) storage of the seeds in ambient temperature at 30 °C and cold storage at -20 °C. It was observed that the rate of germination and germination capacity of M. volkensii seeds increased with extension of storage time. Germination percen-tages were analyzed using R and SAS statistical software. Significance of variation for each month was tested using ANOVA at P≤ 0.05, and means were separated using fisher’s protected least significant difference test. Seed germination after 6 months of storage greatly improved for all storage treatments. The scientific procedure in the present study improved germination from 14 % when seeds were stored for 2 months in open containers at room temperature to 89% when nuts were stored in open containers in room temperature for 6 months. This study is vital in understanding seed storage behavior which is essential in planning and carrying out planting programmes; consequently, enhancing sus-tainable utilization of M. volkensii. Findings on appro-priate storage of nuts and seeds of M. volkensii will help develop a guideline on establishing a seed stock to ensure seed supply at all times even in times of irregular fruiting.
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不同贮藏条件下苦楝种子和坚果贮藏后萌发优化研究
研究了种子(坚果或种子)性质、贮藏条件和贮藏时间对苦楝种子萌发的影响。萌发材料存放2、4、6个月后,分别存放2批种子进行萌发试验。实验室实验设置包括三种处理:1)部分种子提取,其余种子作为坚果储存;2)种子在开放式和封闭式容器中储存;3)种子在室温30°C下储存,在-20°C下冷藏。结果表明,随着贮藏时间的延长,种子的发芽率和萌发能力均有所提高。采用R和SAS统计软件对发芽率进行分析。各月份差异的显著性采用方差分析(ANOVA)检验,P≤0.05,均数采用fisher保护最小显著差异检验。贮藏6个月后,各贮藏处理的种子发芽率均有显著提高。本研究中的科学方法将种子在室温下的开放式容器中储存2个月的发芽率从14%提高到在室温下的开放式容器中储存6个月的89%。这项研究对了解种子储存行为至关重要,这对规划和实施种植计划至关重要;从而提高沃肯氏芽孢杆菌的可持续利用。关于坚果和种子的适当储存的研究结果将有助于制定建立种子库存的指导方针,以确保在任何时候甚至在不规则结果期都有种子供应。
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