STUDY ON PHYTOCHEMICAL SCREENING, TEST FOR INORGANIC ELEMENTS, QUALITATIVE AND QUANTITATIVE CHARACTERIZATION OF PHYTOCONSTITUENTS, TOTAL PHENOL, TOTAL FLAVONOID CONTENT AND ANTI-OXIDANT POTENTIAL OF METHANOL EXTRACT OF SCINDAPSUS AUREUS LEAVES.

S. Kumar
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Abstract

The aim of the present study was to identify the antioxidant potential of methanol extract of Scindapsus aureus leaves. The extract was examined for DPPH free radical scavenging activity, reducing power capacity and for phenol and flavonoid content. Scindapsus aureus is plant belonging to family Araceae. To give a scientific basis for usage of this plant, the leaf extract were appreciated for its antioxidant activity. In the present study we estimate the qualitative analysis and the phytochemical compounds such as steroids, carbohydrates, triterpenoids, coumarins, phenolic compounds, flavonoids, saponins, nthroquinones, Proteins and lipids were screened in leaves of Scindapsus aureus. Quantitative analysis of phenols, flavonoids and Triterpenoids was further performed. Preliminary phytochemical screening of leaves were by methanolic extracts which identified 11 major functional groups, amongst them flavonoid, phytosterols, saponins and coumarins. Additionally inorganic elements like iron, chloride and sulphate were identified by total ash analysis. The extract showed a dose dependent radical scavenging effect in DPPH assay. IC50 for free radicals achieved by the extract is 144.72 μg/ml. The extract showed significant reducing power activity as compared to ascorbic acid and proportionally increased with the increasing concentration of the extract. Increase in World Journal of Pharmaceutical Research SJIF Impact Factor 7.523 Volume 6, Issue 11, 942-955. Research Article ISSN 2277– 7105 *Corresponding Author
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金桂叶甲醇提取物的植物化学筛选、无机元素检测、植物成分、总酚、总黄酮含量及抗氧化活性的定性和定量研究。
本研究的目的是鉴定金杏叶甲醇提取物的抗氧化潜力。测定了提取液对DPPH自由基的清除能力、还原能力、酚和类黄酮含量。金银花是天南星科植物。为了给该植物的使用提供科学依据,对其叶提取物的抗氧化活性进行了评价。本研究对金菖蒲叶中的甾体、碳水化合物、三萜、香豆素、酚类化合物、黄酮类化合物、皂苷类化合物、蒽醌类化合物、蛋白质类化合物和脂类化合物进行了定性分析。进一步对酚类、黄酮类和三萜进行了定量分析。通过叶片甲醇提取物进行初步的植物化学筛选,鉴定出11个主要官能团,其中包括黄酮类、植物甾醇、皂苷和香豆素。此外,无机元素如铁,氯化物和硫酸盐的总灰分分析鉴定。在DPPH实验中,提取物显示出剂量依赖性的自由基清除作用。提取物对自由基的IC50为144.72 μg/ml。与抗坏血酸相比,该提取物显示出显著的降低能量活性,并随着提取物浓度的增加成比例地增加。《世界药物研究杂志》SJIF影响因子7.523,第6卷,第11期,942-955。研究论文:ISSN 2277 - 7105
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