SENSITIVITY OF PATHOGENIC AND OPPORTUNISTIC MICROORGANISMS TO PROBIOTIC STRAINS IN THE LIGHT OF PERSONALIZED PROBIOTIC THERAPY

Abstract

The purpose of the study. To study the sensitivity of clinical strains of pathogenic and opportunistic bacteria and fungi to microorganisms that are part of probiotic preparations. Materials and methods. The sensitivity of 93 cultures of pathogenic and opportunistic microorganisms (UPM) isolated from the feces of patients with intestinal dysbiosis to strains of probiotic bacteria isolated from commercial probiotic preparations was studied: L. plantarum 8RA-3, E. coli M-17, B. subtilis strain VKPM B-10641, E. faecium SF-68, S. boulardii CNCM I-745 by delayed antagonism method. Results. It was found that pathogenic microorganisms showed low and moderate sensitivity to the probiotic strain of L. plantarum. Sensitivity to the probiotic strain of B. subtilis was detected in S. aureus strains, low or completely absent in P. aeruginosa, Klebsiella spp., S. marcescens. The most sensitive to the probiotic strain were clinical strains of E. coli. Conclusion. UPM and pathogenic microorganisms isolated from the gastrointestinal tract of patients with dysbiosis have different sensitivity to probiotic strains: from high to several probiotics to its complete absence. The empirical approach in prescribing a probiotic does not guarantee a therapeutic effect, which makes it expedient to personify the selection of a probiotic based on an assessment of its antimicrobial activity against isolated pathogens.