Canine Amniotic Membrane Derived Mesenchymal Stem Cells- Potential Sources for Regenerative Medicine

“Agriculture for Life, Life for Agriculture” Conference Proceedings Pub Date : 2018-07-01 DOI:10.2478/alife-2018-0071

E. Páll, R. Pop, S. Ciupe, M. Cenariu, I. Groza

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Abstract

Abstract Canine mesenchymal stem cells (MSCs) can be defined with self renew potential and specific differentiation capacity. Amiotic membrane represent an important source of MSCs, which can be harvested by minimally invasive methods. The aim of our study was to evaluate the growth characteristics of canine amniotic membrane derived mesenchymal stem cells. The placenta samples were collected after cesarean section from healthy mixed breed dogs. MSCs isolation was performed using enzymatic method. Isolated cells were cultured in propagation medium: Dulbecco’s Modified Eagle’s Medium/F12 (DMEM/F12, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% antibiotic-antimycotic (Sigma-Aldrich). The medium was changed after 4 days. The cell doubling number, cell proliferation capacity, cell doubling time, daily duplication rate and clonogenic efficacy were evaluated. Our study demonstrate the self renew potential of canine amniotic membrane derived mesenchymal stem cells, and can represent a potential source of stem cells for canine regenerative medicine.

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犬羊膜间充质干细胞-再生医学的潜在来源

犬间充质干细胞(MSCs)具有自我更新潜能和特异性分化能力。Amiotic膜是MSCs的重要来源，可以通过微创方法获得。本研究的目的是评价犬羊膜间充质干细胞的生长特性。选取健康杂交犬剖宫产后的胎盘标本。采用酶法分离MSCs。分离细胞在培养基中培养:Dulbecco 's Modified Eagle 's medium /F12 (DMEM/F12, Gibco)中添加10%胎牛血清(FBS, Gibco)和1%抗生素-抗真菌药(Sigma-Aldrich)。4天后更换培养基。观察细胞倍增数、细胞增殖能力、细胞倍增时间、日复制率和克隆效果。我们的研究证明了犬羊膜间充质干细胞的自我更新潜力，可以代表犬再生医学干细胞的潜在来源。

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“Agriculture for Life, Life for Agriculture” Conference Proceedings

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