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Effect of the Dietary Willow Bark Extract (Salix Alba) on the Caecal Microbial Population of Broilers (14-28 Days) Reared at 32˚C 饲粮中添加柳皮提取物对32℃饲养肉鸡(14 ~ 28日龄)盲肠微生物种群的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0023
M. Sărăcilă, C. Tabuc, T. Panaite, C. Papuc, M. Olteanu, R. Criste
Abstract A feeding trial was performed on 60, Cobb 500 broiler chicks (14-28 days) assigned to 2 groups (C, E) housed in an experimental hall with 32˚C air temperature, 36% humidity and 23 h light regimen. The conventional diet (C), with corn and soybean meal as basic ingredients, had 3082.48 kcal/kg metabolisable energy and 19.99% crude protein. Unlike the diet of C group, the diet of experimental group (E) had 1% willow bark extract (Salix alba). At the age of 28 days, 5 broilers/group were slaughtered and samples of caecal content were collected for bacteriological examination. Compared to group C, the pathogenic bacteria, Enterobacteriacee and Escherichia coli (colony forming units), were significantly (P≤0.05) lower in the caecum content, while the units of lactobacilli were significantly (P≤0.05) higher in group E. Throughout the experimental period, under heat stress, no mortalities were recorded.The inclusion of 1% willow bark extract in broiler diets (14-28 days) reared under heat stress reduced the proliferation of pathogenic bacteria and stimulated the growth of favourable bacteria such as lactobacilli in the gut.
选取60只Cobb 500肉鸡(14 ~ 28日龄),分为2组(C、E),饲养在温度32˚C、湿度36%、光照23 h的试验室内。以玉米和豆粕为基本成分的常规饲粮(C)代谢能为3082.48 kcal/kg,粗蛋白质含量为19.99%。与C组饲粮不同,试验组(E)饲粮中添加1%柳皮提取物。28日龄时,每组屠宰5只肉鸡,采集盲肠内容物样本进行细菌学检查。与C组相比,e组盲肠致病菌肠杆菌和大肠杆菌(集落形成单位)含量显著(P≤0.05)低于C组,乳酸杆菌含量显著(P≤0.05)高于C组。在热应激条件下饲养的肉鸡饲粮(14-28日龄)中添加1%柳树皮提取物可减少致病菌的增殖,促进肠道有益菌(如乳酸菌)的生长。
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引用次数: 6
Antibacterial Activity of Magnetite Nanoparticles Coated with Bee Pollen Extracts 蜂花粉提取物包被磁性纳米颗粒的抗菌活性研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0091
R. Spulber, Carmen Chifiriuc, M. Fleancu, O. Popa, N. Babeanu
Abstract In a nanobiotechnology world with many applications in biomedicine, a novel combination of inorganic-organic materials is needed to prove a novel functionality. Natural compounds from bee pollen extract coated on magnetite nanoparticles could open up a new way in apitherapy field. Iron oxide nanoparticles have proved special requirements for biological applications like superparamagnetic properties, high biocompatibility and nontoxic material. Magnetite nanoparticles functionalized with natural bioactive substances extracted from bee pollen have been characterised and investigated for antimicrobial activity. Previous findings demonstrate that magnetite nanoparticles (MNPs) and pollen ethanolic extracts (PEE) exhibited antimicrobial activity against a large antimicrobial spectrum, including Grampositive, Gram-negative and antifungal microorganisms.
摘要纳米生物技术在生物医学领域有着广泛的应用,因此需要一种无机-有机材料的新型组合来证明一种新的功能。从蜂花粉提取物中提取的天然化合物包覆在纳米磁铁矿上,为蜂疗领域开辟了新的途径。氧化铁纳米颗粒已被证明是生物应用的特殊要求,如超顺磁性,高生物相容性和无毒材料。从蜂花粉中提取的天然生物活性物质功能化的磁铁矿纳米颗粒已经被表征并研究了其抗菌活性。先前的研究结果表明,磁铁矿纳米颗粒(MNPs)和花粉乙醇提取物(PEE)对包括革兰氏阳性、革兰氏阴性和抗真菌微生物在内的多种微生物具有抗菌活性。
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引用次数: 6
Global Food Security Index’s Reflections to Balkan Countries 全球粮食安全指数对巴尔干国家的启示
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0030
Ö. Turan, S. Gurluk, Edibe Issi
Abstract Policies for ensuring food security have gained considerable importance in recent years. The policy success of the countries at this lower level is very important in ensuring food safety. It is also important to determine where the countries take place in food security. In determining the success of countries The Global Food Security Index (GFSI) takes on an important role. In order to prepare the index different subsections of food security such as food availability, food accessibility, food quality and safety are calculated using different weights. This index is re-prepared every year and allows the user to make changes using different weights and calculating rankings again. It will also include recommendations on agriculture and food policy that can be taken to improve their places in the food security index. In this study, an analysis of GFSI for 2017 will be made for Turkey, Romania and Bulgaria. It also includes recommendations on agriculture and food policy of countries for these countries to increase their rankings on the GFSI.
近年来,确保粮食安全的政策变得相当重要。这些国家在这一较低水平上的政策成功对确保食品安全非常重要。确定各国在粮食安全方面的地位也很重要。在决定一个国家是否成功的过程中,全球粮食安全指数(GFSI)发挥着重要作用。为了编制指数,粮食安全的不同部分,如粮食供应、粮食可及性、食品质量和安全,使用不同的权重进行计算。该指数每年都会重新编制,并允许用户使用不同的权重进行更改,并再次计算排名。报告还将包括有关农业和粮食政策的建议,这些建议可以用来提高它们在粮食安全指数中的排名。在本研究中,将对2017年土耳其、罗马尼亚和保加利亚的GFSI进行分析。它还包括对这些国家的农业和粮食政策的建议,以提高它们在GFSI中的排名。
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引用次数: 6
Climate Change’s Impact on Sandy Soils and on the Grafted Watermelons Adaptation 气候变化对沙质土壤及西瓜嫁接适应的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0053
Gabriela Mihaela Ciupureanu, E. Ciuciuc, A. Diaconu, A. Ciuca, D. Popa
Abstract Starting from the fact that the principles of agro-ecology become fundamental principles for the development of a green economy, especially in the context of current climate change, the effective capitalization of the ecological conditions of an agricultural area is one of the main objectives of agricultural science and practice. Identifying and formulating adaptive technological solutions can guide any producer to capitalize different climate and soil conditions. The sandy soils in Southern Oltenia offer less favourable ecological conditions, and the cultivation of watermelons is now profitable enough for such conditions. Growers are, however, interested in getting the most productive yields, early and profitable, even under the conditions of climate change. The current paper quantifies the grafting of watermelons in the conditions of the sandy soils of Dăbuleni, with poor soil supply, with meteorological drought phenomena and agricultural drought risk, in terms of quality and quantity of production under the climatic conditions in 2015-2017. The results, correlated with the climatic conditions, recommend the cultivation of grafted watermelons, offering the producers in the area a niche of ecological adaptation, ensuring the resistance of plants to abiotic, thermo-hydric stress factors, and improving the resistance to low temperatures, heat and drought.
从农业生态学原则成为发展绿色经济的基本原则这一事实出发,特别是在当前气候变化的背景下,农业地区生态条件的有效资本化是农业科学和实践的主要目标之一。确定和制定适应性技术解决方案可以指导任何生产者利用不同的气候和土壤条件。南奥特尼亚的沙质土壤提供了不太有利的生态条件,在这种条件下种植西瓜现在已经足够有利可图了。然而,即使在气候变化的条件下,种植者也希望尽早获得最高的产量和利润。本文从2015-2017年气候条件下,定量分析了在土壤供应差、存在气象干旱现象和农业干旱风险的dunigerbuleni沙质土壤条件下西瓜的嫁接质量和产量。研究结果与气候条件相关联,推荐嫁接西瓜的种植,为该地区的生产者提供了生态适应的生态位,保证了植物对非生物、热水胁迫因素的抗性,提高了植物对低温、高温和干旱的抗性。
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引用次数: 0
Researches Regarding New and Improved Technologies for Sunflower and Sorghum Crops in the Context of Climate Changes in Dobrogea Region 气候变化背景下多布罗格亚地区向日葵和高粱作物新改良技术研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0012
D. Manole, V. Jinga, A. Giumba, R. Dudoiu, S. Cristea
Abstract At Sport Agra in Amzacea, in the last few years there have been experimented new sunflower and sorghum crop technologies designed to face the current climate changes. These technologies for the intended crops include the following elements: changing the sowing epoch with one month before the usual period recommended by classical technologies; application of herbicides in order to control both weeds and Orobanche cumana Wallr. parasite in plots cultivated with various hybrids from Syngenta and Limagrain companies; application of last generation fungicides during the vegetation period, which will reduce the attack of the main crop pathogens; screening of hybrids with good behavior towards the main pest agent of area - Orobanche cumana parasite, which cause important yield losses in the south east past of Romania. There were tested 8 hybrids from Syngenta Company, 6 hybrids from Limagrain and 4 hybrids from NARDI Fundulea. The yields obtained for most of the hybrids were over 4 t/ha. For sorghum crop, there were performed various experiments such as: changing the sowing period - beginning of April in order to benefit from the soil’s humidity at 4-5 cm depth boosting the germination process; choosing early hybrids in order to avoid the drought season which starts in June; applying adequate crop protection treatments, with pre-emergent and postemergent herbicides and last generation insecticides. The obtained production from sorghum crop were over 10 t/ha for most of the varieties tested.
在过去的几年里,在Amzacea的Sport Agra,已经试验了新的向日葵和高粱作物技术,旨在面对当前的气候变化。这些针对预期作物的技术包括以下内容:改变播种期,将传统技术建议的播种期提前一个月;除草剂的应用,以控制杂草和美洲红腹蛇。先正达(Syngenta)和利马格兰(Limagrain)公司的各种杂交种种植的地块上的寄生虫;在生长期施用上一代杀菌剂,可减少作物主要病原菌的侵袭;筛选对罗马尼亚东南部地区主要有害生物——罗马尼亚斑蝽寄生虫具有良好抗性的杂交种。先正达8个,利马格兰6个,NARDI Fundulea 4个。大多数杂交种的产量都在4吨/公顷以上。对高粱作物进行了不同的试验,如:改变播种期- 4月初,以利用土壤4-5 cm深度的湿度促进发芽过程;选择早期杂交品种,以避开6月开始的干旱季节;采用适当的作物保护处理,使用苗期前和苗期后的除草剂和上一代杀虫剂。大多数高粱品种的产量均在10吨/公顷以上。
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引用次数: 5
Assessing the Inter-Relations Between Fish Health and Stock Status of the Main Commercial Fish Species 鱼类健康与主要商业鱼类种群状况的相互关系评价
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0025
Aurelia Țoțoiu, N. Patriche
Abstract This paper aims to show the importance of knowing the health status of fish populations in their natural environment and its influence on fish stocks at the Romanian coast. To assess the interrelationship between fish health status and the state of stocks, the following fish species: turbot, sprat, anchovies and horse mackerel were analysed from the pathological point of view. Pathological analyses performed between 2015 and 2017 revealed the presence of infections caused by bacteria of the genus Aeromonas and Vibrio and parasitic diseases Trichodinosis, Botriocephalosis and Nematodosis. The presence of these diseases in natural fish populations may represent a real danger to the state of existing stocks, but also to their evolution.
摘要本文旨在表明了解鱼类种群在自然环境中的健康状况及其对罗马尼亚海岸鱼类种群的影响的重要性。为了评估鱼类健康状况与种群状况之间的相互关系,从病理学角度分析了下列鱼类:大比目鱼、鲽鱼、凤尾鱼和马鲛鱼。2015年至2017年期间进行的病理分析显示,存在由气单胞菌属和弧菌属细菌引起的感染,以及寄生虫病Trichodinosis, botricephalosis和Nematodosis。这些疾病在天然鱼类种群中的存在可能对现有鱼类种群的状况构成真正的危险,但也可能对它们的进化构成真正的危险。
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引用次数: 1
Study of Heavy Metals in the Soils of Two Localities in Dolj County 多丽县两地土壤重金属含量研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0052
G. Buzatu, A. M. Dodocioiu
Abstract The study was conducted in two neighboring localities located in the northern county of Dolj, namely Murgasi and Bulzesti. The purpose of the study was twofold: namely, the study of heavy metal loading in order to identify possible pollution areas and to know the soil content of these localities in heavy metals in order to be able to give the verdict on the use of sludge from Craiova wastewater treatment plant as fertilizer on these soils. In order to determine the suitability of sewage sludge from the Craiova wastewater treatment plant as a fertilizer, physical and chemical properties and heavy metal content of the soils in these areas were analysed, as well as the chemical composition of sludge, according to Order 344/2004 of the Ministry of Environment and Water Management of Romania, respectively 13 physical and chemical parameters of the soils in these localities and the chemical composition of the sludge and respectively the quantity of heavy metals to be introduced annually into the soil by using it.
本研究在位于Dolj北部的穆尔加西(Murgasi)和布尔泽斯蒂(Bulzesti)两个相邻的地方进行。该研究的目的有两个:即,研究重金属负荷,以确定可能的污染区域,并了解这些地区的土壤重金属含量,以便能够对使用克拉约瓦污水处理厂的污泥作为这些土壤的肥料作出裁决。根据罗马尼亚环境和水管理部第344/2004号命令,为了确定克拉约瓦污水处理厂的污水污泥是否适合作为肥料,对这些地区土壤的物理和化学性质、重金属含量以及污泥的化学成分进行了分析。分别分析了这些地区土壤的13个理化参数和污泥的化学成分,以及利用污泥每年向土壤中引入的重金属量。
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引用次数: 0
Epidemiological Observations on Infectious Pathology of Goats in the Northeast Area of Romania 罗马尼亚东北部山羊感染病理学的流行病学观察
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0069
I. Mihai, E. Velescu, O. Tanase
Abstract The goat flock in our country is steadily increasing, so that if 1072013 animals were reported in 2007, they were 1804478 in December 2016 that reflecting the growing interest of breeders for this species. This paper aims to highlight the dynamics of infectious pathology in the goat population in the Northeast region of Romania during 2014-2017, the factors that led to the emergence of diseases, as well as the prevention and control measures. The most common diseases are those of the respiratory, digestive, reproductive and locomotors apparatus produced by infectious pathogens such as Pasteurella spp., Artrithis Encephalitis Virus, Orf Virus, Mycoplasma agalactiae, Dichelobacter nodosus and Fusobacterium necrophorum. Research has led to establishment of the prevalence of infectious diseases in goats, so the results show that the most affected breeds are specialized breeds: Saanen and French Alpine, kids being more sensitive compared to adult animals. Knowledge of the epidemiological situation is the basis for proposing specific control and / or prevention plans that allow the design, management and evaluation of goat health programs.
我国山羊存栏数量稳步增长,从2007年的1072013只增加到2016年12月的1804478只,反映出饲养者对山羊存栏的兴趣日益浓厚。本文旨在分析2014-2017年罗马尼亚东北地区山羊种群的感染病理动态、导致疾病发生的因素以及预防和控制措施。最常见的疾病是由感染性病原体如巴氏杆菌、关节炎脑炎病毒、Orf病毒、无乳支原体、结节双杆菌和坏死梭杆菌等引起的呼吸、消化、生殖和运动器官的疾病。研究已经确定了山羊传染病的流行情况,因此结果表明,受影响最严重的品种是特殊品种:Saanen和French Alpine,与成年动物相比,儿童更敏感。了解流行病学情况是提出具体控制和/或预防计划的基础,从而可以设计、管理和评估山羊健康规划。
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引用次数: 3
Farms Typologies and Their Impact on the Development of Rural Areas in Moldova 摩尔多瓦农场类型及其对农村地区发展的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0028
L. Cimpoieș, E. Semionova
Abstract For Moldova’s agriculture are characterized the existence of many small family farms and only a small number of corporate holdings (limited liabilities companies, agricultural production cooperatives etc). Most of individual farms, small by size produce only for own family consumption and cannot find their way to the market place. The accentuated poverty makes rural economy to flow more and more to a natural subsistence economy, isolating itself from the market economy. The goal of this paper is to delimitate different types of farms based on selected indicators and to appreciate their level of development. Differences in farms levels of development are appreciated through cluster analysis. The research includes the analysis of farms survey data. This research was carried as a part of the Academy of Sciences project “Sustainable rural development in Republic of Moldova in the context of EU accession”. In the project were surveyed 938 individual farms from nine districts across the country. As a result, we distinguish three types of farms: natural, family and market household. The cluster analysis allows to characterize the farms level development, to determine the main priority directions, and to elaborate measures for the individual farms further sustainable development.
摩尔多瓦农业的特点是存在许多小型家庭农场,只有少数公司控股(有限责任公司,农业生产合作社等)。大多数规模较小的个体农场只生产供自己家庭消费的产品,无法找到进入市场的途径。贫困的加剧使农村经济越来越趋向于自然自给经济,与市场经济相隔绝。本文的目的是根据选定的指标划分不同类型的农场,并了解其发展水平。通过聚类分析可以了解农场发展水平的差异。这项研究包括对农场调查数据的分析。这项研究是作为科学院项目“加入欧盟背景下摩尔多瓦共和国的可持续农村发展”的一部分进行的。该项目对全国9个地区的938个个体农场进行了调查。因此,我们将农场分为三种类型:自然农场、家庭农场和市场家庭农场。通过聚类分析,可以表征养殖场的发展水平,确定主要优先发展方向,并为个体养殖场进一步的可持续发展制定措施。
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引用次数: 1
Biochemical Investigations of Different Mushroom Species for Their Biotechnological Potential 不同菌种生物技术潜力的生化研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0088
M. Nicolcioiu, G. Popa, F. Matei
Abstract Among bioactive constituents occurring in mushrooms, phenolic compounds focus attention due to their antioxidant activity. Also, a special attention is given to mushrooms secreting extra cellular enzymes such as lignin peroxidases (LiP), manganese peroxidase (MnP) and laccase (Lac), enzymes which can be used in biodegradation processes. The aim of the present study was to investigate ten mushroom species for total phenolic compounds, lignocellulolytic enzymes and for their synthetic dyes decolourisation potential. For this purpose, 70% ethanol extracts of ten dried mushrooms were analysed using spectrophotometric methods. The results revealed that total phenolic compounds in the extracts were the highest in A. campestris, P. ostreatus var. Florida and T. versicolor. Laccase activity showed high values in extracts from P. ostreatus var. Florida, A. campestris, L. edodes, and G. applanatum. Lignin peroxidases (LiP) activity showed high values in extracts from A. campestris, F. velutipes, P. ostreatus var. Florida and T. versicolor, whereas manganese peroxidase (MnP) activity was highest in extracts from P. ostreatus var. Florida, A. campestris and G. applanatum. It was found that some of the fungal extracts showed high activities in decolorizing of synthetic dyes.
在蘑菇中存在的生物活性成分中,酚类化合物因其抗氧化活性而备受关注。此外,还特别关注蘑菇分泌的细胞外酶,如木质素过氧化物酶(LiP)、锰过氧化物酶(MnP)和漆酶(Lac),这些酶可用于生物降解过程。本研究的目的是研究10种蘑菇的总酚类化合物、木质纤维素水解酶及其合成染料的脱色潜力。为此,采用分光光度法对10种干蘑菇的70%乙醇提取物进行了分析。结果表明,总酚类化合物含量最高的品种为野田葵、佛罗里达野田葵和花斑田葵。漆酶活性较高的分别为佛罗里达、野田葵、油桐和扁桃提取物。木质素过氧化物酶(LiP)活性最高的是油桐、黄皮油桐、桃叶油桐和紫叶油桐,而锰过氧化物酶(MnP)活性最高的是桃叶油桐、桃叶油桐和扁桃。发现部分真菌提取物对合成染料有较高的脱色活性。
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引用次数: 5
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